We are analyzing https://link.springer.com/article/10.1007/s11010-009-0266-3.

Title:
Telomerase activity and hepatic functions of rat embryonic liver progenitor cell in nanoscaffold-coated model bioreactor | Molecular and Cellular Biochemistry
Description:
Presently, there is growing interest on telomerase activity in all cells (somatic cells, stem cells, cancerous cells and others) since this activity is associated with cellular changes such as proliferation, differentiation, immortalization, cell injury and ageing. Telomerase activity is absent in most of the somatic cells but present in over 90% of cancerous cells and other immortalized cell lines. In our present study, we cultured a rat embryonal liver progenitor cell line RLC-18 in a self-assembly nanostructured scaffold-coated bioreactor (NCB), collagen-coated plates (CCP) and uncoated plates (UP), and evaluated changes of telomerase activity by non radioactive techniques (Telo TAGGG Telomerase PCR ELISA, cell proliferation based on mitochondria number by MTT assay and hepatic functions such as albumin secretion, urea metabolism, Cytochrome P450 activity like ethoxyresorufin-O-deethylase (EROD) activity. We found less telomerase activity and less cell proliferation, but more hepatic functions on the NCB than on the CCP and UP. Our data support the concept that cell-scaffold interaction may play a significant in controlling the telomerase activity as well as enhanced hepatic functions. Although our present study does not focus on the exact mechanism of telomerase regulation, our result may provide basic clues on cell differentiation whereby telomerase activity inhibits differentiation of cells as in the rat embryonic liver cell line, may be regulated by cell–scaffold interaction and where there is less proliferation, cells perform enhanced hepatic functions, thereby implying that bioartificial liver support may be possible.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't tell how the site generates income.

Some websites aren't about earning revenue; they're built to connect communities or raise awareness. There are numerous motivations behind creating websites. This might be one of them. Link.springer.com might have a hidden revenue stream, but it's not something we can detect.

Keywords {🔍}

google, scholar, article, pubmed, cas, liver, cell, cells, telomerase, activity, hepatic, bader, peptide, bioreactor, selfassembling, bioartificial, zhang, differentiation, scaffolds, human, functions, rat, tissue, stem, support, access, culture, proliferation, hepatocytes, porcine, frühauf, biotechnol, hydrogel, privacy, cookies, content, cellular, progenitor, model, study, engineering, failure, cancer, extracorporeal, hepatocyte, vitro, eng, biomaterials, semino, res,

Topics {✒️}

melt-electrospun poly-ε-caprolactone scaffolds month download article/chapter small-scale bioreactor based matrix metalloproteinase-sensitive substrate nanoscaffold-coated model bioreactor peptide-based biomaterial scaffolds full article pdf anti-telomerase rna component liver stem cells albumin secretion flat membrane bioreactor stem cell properties small scale bioreactor extracorporeal liver support cellular biochemistry aims bioartificial liver support porcine liver cells bioartificial liver systems peptide nanofiber scaffold rat liver cells conditions privacy policy assembling peptide hydrogel stable macroscopic membrane flat membrane bioreactors extracorporeal bioartificial liver assembling peptide scaffolds enhanced hepatic functions assembling peptide scaffold fetal liver transplantation related subjects acute liver failure severe liver failure dimensional peptide scaffolds privacy choices/manage cookies fulminant hepatic failure cell-scaffold interaction cell–scaffold interaction urea metabolism oxygen consumption characteristics differentiation potential tissue engineering applications dimensional hydrogel scaffolds radial-flow bioreactor mini-bioreactor utilisation porcine hepatectomy model immortal human cells porcine hepatocytes stem cells check access instant access

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Telomerase activity and hepatic functions of rat embryonic liver progenitor cell in nanoscaffold-coated model bioreactor
         description:Presently, there is growing interest on telomerase activity in all cells (somatic cells, stem cells, cancerous cells and others) since this activity is associated with cellular changes such as proliferation, differentiation, immortalization, cell injury and ageing. Telomerase activity is absent in most of the somatic cells but present in over 90% of cancerous cells and other immortalized cell lines. In our present study, we cultured a rat embryonal liver progenitor cell line RLC-18 in a self-assembly nanostructured scaffold-coated bioreactor (NCB), collagen-coated plates (CCP) and uncoated plates (UP), and evaluated changes of telomerase activity by non radioactive techniques (Telo TAGGG Telomerase PCR ELISA, cell proliferation based on mitochondria number by MTT assay and hepatic functions such as albumin secretion, urea metabolism, Cytochrome P450 activity like ethoxyresorufin-O-deethylase (EROD) activity. We found less telomerase activity and less cell proliferation, but more hepatic functions on the NCB than on the CCP and UP. Our data support the concept that cell-scaffold interaction may play a significant in controlling the telomerase activity as well as enhanced hepatic functions. Although our present study does not focus on the exact mechanism of telomerase regulation, our result may provide basic clues on cell differentiation whereby telomerase activity inhibits differentiation of cells as in the rat embryonic liver cell line, may be regulated by cell–scaffold interaction and where there is less proliferation, cells perform enhanced hepatic functions, thereby implying that bioartificial liver support may be possible.
         datePublished:2009-10-09T00:00:00Z
         dateModified:2009-10-09T00:00:00Z
         pageStart:137
         pageEnd:149
         sameAs:https://doi.org/10.1007/s11010-009-0266-3
         keywords:
            Albumin secretion
            Bioreactor
            Telomerase
            RLC-18 cell line
            Urea synthesis
            Biochemistry
            general
            Cardiology
            Cancer Research
            Medical Biochemistry
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig6_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig7_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig8_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig9_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig10_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig11_HTML.gif
         isPartOf:
            name:Molecular and Cellular Biochemistry
            issn:
               1573-4919
               0300-8177
            volumeNumber:336
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer US
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Shibashish Giri
               affiliation:
                     name:Biomedical-Biotechnological Center (BBZ)
                     address:
                        name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Karen Nieber
               affiliation:
                     name:University Leipzig
                     address:
                        name:Institute of Pharmacy, Pharmacology for Natural Sciences, University Leipzig, Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ali Acikgöz
               affiliation:
                     name:Biomedical-Biotechnological Center (BBZ)
                     address:
                        name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Sanja Pavlica
               affiliation:
                     name:Biomedical-Biotechnological Center (BBZ)
                     address:
                        name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Mario Keller
               affiliation:
                     name:Biomedical-Biotechnological Center (BBZ)
                     address:
                        name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Augustinus Bader
               affiliation:
                     name:Biomedical-Biotechnological Center (BBZ)
                     address:
                        name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Telomerase activity and hepatic functions of rat embryonic liver progenitor cell in nanoscaffold-coated model bioreactor
      description:Presently, there is growing interest on telomerase activity in all cells (somatic cells, stem cells, cancerous cells and others) since this activity is associated with cellular changes such as proliferation, differentiation, immortalization, cell injury and ageing. Telomerase activity is absent in most of the somatic cells but present in over 90% of cancerous cells and other immortalized cell lines. In our present study, we cultured a rat embryonal liver progenitor cell line RLC-18 in a self-assembly nanostructured scaffold-coated bioreactor (NCB), collagen-coated plates (CCP) and uncoated plates (UP), and evaluated changes of telomerase activity by non radioactive techniques (Telo TAGGG Telomerase PCR ELISA, cell proliferation based on mitochondria number by MTT assay and hepatic functions such as albumin secretion, urea metabolism, Cytochrome P450 activity like ethoxyresorufin-O-deethylase (EROD) activity. We found less telomerase activity and less cell proliferation, but more hepatic functions on the NCB than on the CCP and UP. Our data support the concept that cell-scaffold interaction may play a significant in controlling the telomerase activity as well as enhanced hepatic functions. Although our present study does not focus on the exact mechanism of telomerase regulation, our result may provide basic clues on cell differentiation whereby telomerase activity inhibits differentiation of cells as in the rat embryonic liver cell line, may be regulated by cell–scaffold interaction and where there is less proliferation, cells perform enhanced hepatic functions, thereby implying that bioartificial liver support may be possible.
      datePublished:2009-10-09T00:00:00Z
      dateModified:2009-10-09T00:00:00Z
      pageStart:137
      pageEnd:149
      sameAs:https://doi.org/10.1007/s11010-009-0266-3
      keywords:
         Albumin secretion
         Bioreactor
         Telomerase
         RLC-18 cell line
         Urea synthesis
         Biochemistry
         general
         Cardiology
         Cancer Research
         Medical Biochemistry
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig6_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig7_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig8_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig9_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig10_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11010-009-0266-3/MediaObjects/11010_2009_266_Fig11_HTML.gif
      isPartOf:
         name:Molecular and Cellular Biochemistry
         issn:
            1573-4919
            0300-8177
         volumeNumber:336
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Shibashish Giri
            affiliation:
                  name:Biomedical-Biotechnological Center (BBZ)
                  address:
                     name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Karen Nieber
            affiliation:
                  name:University Leipzig
                  address:
                     name:Institute of Pharmacy, Pharmacology for Natural Sciences, University Leipzig, Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ali Acikgöz
            affiliation:
                  name:Biomedical-Biotechnological Center (BBZ)
                  address:
                     name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Sanja Pavlica
            affiliation:
                  name:Biomedical-Biotechnological Center (BBZ)
                  address:
                     name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mario Keller
            affiliation:
                  name:Biomedical-Biotechnological Center (BBZ)
                  address:
                     name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Augustinus Bader
            affiliation:
                  name:Biomedical-Biotechnological Center (BBZ)
                  address:
                     name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Molecular and Cellular Biochemistry
      issn:
         1573-4919
         0300-8177
      volumeNumber:336
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Biomedical-Biotechnological Center (BBZ)
      address:
         name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
         type:PostalAddress
      name:University Leipzig
      address:
         name:Institute of Pharmacy, Pharmacology for Natural Sciences, University Leipzig, Leipzig, Germany
         type:PostalAddress
      name:Biomedical-Biotechnological Center (BBZ)
      address:
         name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
         type:PostalAddress
      name:Biomedical-Biotechnological Center (BBZ)
      address:
         name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
         type:PostalAddress
      name:Biomedical-Biotechnological Center (BBZ)
      address:
         name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
         type:PostalAddress
      name:Biomedical-Biotechnological Center (BBZ)
      address:
         name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Shibashish Giri
      affiliation:
            name:Biomedical-Biotechnological Center (BBZ)
            address:
               name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Karen Nieber
      affiliation:
            name:University Leipzig
            address:
               name:Institute of Pharmacy, Pharmacology for Natural Sciences, University Leipzig, Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Ali Acikgöz
      affiliation:
            name:Biomedical-Biotechnological Center (BBZ)
            address:
               name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Sanja Pavlica
      affiliation:
            name:Biomedical-Biotechnological Center (BBZ)
            address:
               name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Mario Keller
      affiliation:
            name:Biomedical-Biotechnological Center (BBZ)
            address:
               name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
               type:PostalAddress
            type:Organization
      name:Augustinus Bader
      affiliation:
            name:Biomedical-Biotechnological Center (BBZ)
            address:
               name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
      name:Institute of Pharmacy, Pharmacology for Natural Sciences, University Leipzig, Leipzig, Germany
      name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
      name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
      name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
      name:Department of Cell Technologies and Applied Stem Cell Biology, Biomedical-Biotechnological Center (BBZ), Leipzig, Germany
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(210)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.8s.

LINK . SPRINGER . COM {}