We are analyzing https://link.springer.com/article/10.1007/s10911-005-2542-5.

Title:
Survival of Mammary Stem Cells in Suspension Culture: Implications for Stem Cell Biology and Neoplasia | Journal of Mammary Gland Biology and Neoplasia
Description:
There is increasing evidence that a variety of neoplasms including breast cancer may result from transformation of normal stem and progenitor cells. In the past, isolation and characterization of mammary stem cells has been limited by the lack of suitable culture systems able to maintain these cells in an undifferentiated state in vitro. We have recently described a culture system in which human mammary stem and progenitor cells are able to survive in suspension and produce spherical colonies composed of both stem and progenitor cells. Recent observation that adult stem cells from other tissues may also retain the capacity for growth under anchorage independent conditions suggests a common underlying mechanism. We propose that this mechanism involves the interaction between the canonical Wnt signal pathway and E-cadherin. The Wnt pathway has been implicated in normal stem cell self-renewal in vivo. Furthermore, there is evidence that deregulation of this pathway in the mammary gland and other organs may play a key role in carcinogenesis. Thus, the development of in vitro suspension culture systems not only provides an important new tool for the study of mammary cell biology, but also may have important implications for understanding key molecular pathways in both normal and neoplastic stem cells.
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Keywords {🔍}

google, scholar, pubmed, cas, stem, cells, article, cell, mammary, cancer, human, breast, gland, adult, wnt, development, progenitor, biol, neural, signaling, epithelial, nature, normal, dev, dontu, clarke, sci, biology, neoplasia, proc, acad, function, wicha, vitro, pathway, selfrenewal, differentiation, gene, mouse, nat, natl, res, usa, privacy, cookies, content, journal, culture, growth, access,

Topics {✒️}

month download article/chapter multipotent stem/progenitor cells mi-2/nurd complex subunit nestin-negative precursor cell beta-catenin binding domain stem cell biology delta-notch signaling controls nestin-egfp transgenic mice neural stem cells neural stem cell mammary cell biology adult stem cells human mammary stem mammary stem cells mammary epithelial stem mammary gland biology cancer stem cells cancer stem cell cns stem cells mammary epithelial cells renewal differentiation access pluripotent stem cells cadherin-catenin adhesion system adult mammalian forebrain neoplastic stem cells haematopoietic stem cells gastrointestinal stem cells mammary progenitor cells normal stem cell adult human brain cell-fate determination full article pdf apc tumor suppressor mammary gland development adult mouse brain human brain tumors privacy choices/manage cookies progenitor cell responsiveness related subjects beta-catenin signaling primitive hematopoietic cell human leukemic development wnt signalling pathways adult small intestine neoplastic mammary epithelium cells expressing astroglial granulocyte–macrophage progenitors comprehensive cancer center epithelial bud development neural stem

Questions {❓}

The evolving concept of a stem cell: Entity or function?
Wnt signaling in breast cancer: Have we come full circle?
Wnt signalling: pathway or network?

Schema {🗺️}

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      description:There is increasing evidence that a variety of neoplasms including breast cancer may result from transformation of normal stem and progenitor cells. In the past, isolation and characterization of mammary stem cells has been limited by the lack of suitable culture systems able to maintain these cells in an undifferentiated state in vitro. We have recently described a culture system in which human mammary stem and progenitor cells are able to survive in suspension and produce spherical colonies composed of both stem and progenitor cells. Recent observation that adult stem cells from other tissues may also retain the capacity for growth under anchorage independent conditions suggests a common underlying mechanism. We propose that this mechanism involves the interaction between the canonical Wnt signal pathway and E-cadherin. The Wnt pathway has been implicated in normal stem cell self-renewal in vivo. Furthermore, there is evidence that deregulation of this pathway in the mammary gland and other organs may play a key role in carcinogenesis. Thus, the development of in vitro suspension culture systems not only provides an important new tool for the study of mammary cell biology, but also may have important implications for understanding key molecular pathways in both normal and neoplastic stem cells.
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