We are analyzing https://link.springer.com/article/10.1007/s10735-025-10362-1.

Title:
The role of RAP2 in regulation of cell volume on bone marrow mesenchymal stem cell fate determination | Journal of Molecular Histology
Description:
The extracellular matrix guides cell behavior through mechanical properties, which plays a role in determining cell function and can even influence stem cell fate. Compared with adherent culture, the three-dimensional culture environment is closer to the growth conditions in vivo, but is limited by standardization of material properties and observation and measurement methods. Therefore, it is necessary to study the relationship among the three-dimensional morphological characteristics of cells, cytoskeleton, and stem cell differentiation under adherent culture conditions. Here, we control the cell volume by adjusting the cell density, microfilament cytoskeleton tension, and osmotic pressure of the culture environment, and analyze the cell morphological features and differentiation to the osteoblastic and adipogenic lineages. Based on the in vitro and in vivo results, we identify cell volume as the true reflection of the cytoskeleton tension under stress stimuli compared with cell spreading area. By adjusting cell volume, cytoskeletal tension and cell differentiation can be regulated without affecting cell spreading area. Further study shows that the Ras-related small GTPase RAP2 inhibits the activity of mechanical transducers Lamin A/C and YAP1, playing an important role in cell volume regulation of cell differentiation. In summary, our results support the close relationship between cell volume and cytoskeleton tension. The regulatory role of cell volume on cell differentiation is modulated, at least in part, by RAP2-related mechanosensitive pathways. Our insights into how cell volume regulates cell differentiation may build a bridge between two-dimensional and three-dimensional mechanical studies in cell biology.
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

cell, article, pubmed, stem, google, scholar, cas, cells, central, volume, fate, differentiation, httpsdoiorgs, mesenchymal, regulates, matrix, spreading, research, regulation, mechanical, culture, cellular, nuclear, national, stomatology, data, role, rap, zhang, threedimensional, cytoskeleton, tension, lamin, access, periodontal, httpsdoiorgpnas, stiffness, china, center, privacy, cookies, content, journal, bone, guo, yap, science, hydrogel, mater, biomaterials,

Topics {✒️}

rhoa/rock-yap/taz axis regulates month download article/chapter stem cell fate rap2-related mechanosensitive pathways oliver-de la cruz mesenchymal stem cells stem cell differentiation stem cells remains stem cell renewal full article pdf adult stem cells stem cells cultured cell–cell contact studying bone remodeling force-induced h2s privacy choices/manage cookies human periodontal ligament cell communication signaling stiffness mediated-mechanosensation thrombospondin-1/integrin/yap human embryo compaction identify cell volume cell morphological features single-cell profiling cell-geometry-dependent article zhou periodontal ligament cells matrix-directed differentiation cell spreading area cell biology facilitate cell spreading matrix mechanotransduction mediated cell fate holds exclusive rights accepted manuscript version cell volume regulation tissue cells feel direct influence nuclear lamin proteins adjusting cell volume cell volume change dimensional morphological characteristics determining cell function matrix remodeling controls study cell volume stem cells repairing periodontal defects west china hospital rap2 mediates mechanoresponses curr biology

Schema {🗺️}

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         description:The extracellular matrix guides cell behavior through mechanical properties, which plays a role in determining cell function and can even influence stem cell fate. Compared with adherent culture, the three-dimensional culture environment is closer to the growth conditions in vivo, but is limited by standardization of material properties and observation and measurement methods. Therefore, it is necessary to study the relationship among the three-dimensional morphological characteristics of cells, cytoskeleton, and stem cell differentiation under adherent culture conditions. Here, we control the cell volume by adjusting the cell density, microfilament cytoskeleton tension, and osmotic pressure of the culture environment, and analyze the cell morphological features and differentiation to the osteoblastic and adipogenic lineages. Based on the in vitro and in vivo results, we identify cell volume as the true reflection of the cytoskeleton tension under stress stimuli compared with cell spreading area. By adjusting cell volume, cytoskeletal tension and cell differentiation can be regulated without affecting cell spreading area. Further study shows that the Ras-related small GTPase RAP2 inhibits the activity of mechanical transducers Lamin A/C and YAP1, playing an important role in cell volume regulation of cell differentiation. In summary, our results support the close relationship between cell volume and cytoskeleton tension. The regulatory role of cell volume on cell differentiation is modulated, at least in part, by RAP2-related mechanosensitive pathways. Our insights into how cell volume regulates cell differentiation may build a bridge between two-dimensional and three-dimensional mechanical studies in cell biology.
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      headline:The role of RAP2 in regulation of cell volume on bone marrow mesenchymal stem cell fate determination
      description:The extracellular matrix guides cell behavior through mechanical properties, which plays a role in determining cell function and can even influence stem cell fate. Compared with adherent culture, the three-dimensional culture environment is closer to the growth conditions in vivo, but is limited by standardization of material properties and observation and measurement methods. Therefore, it is necessary to study the relationship among the three-dimensional morphological characteristics of cells, cytoskeleton, and stem cell differentiation under adherent culture conditions. Here, we control the cell volume by adjusting the cell density, microfilament cytoskeleton tension, and osmotic pressure of the culture environment, and analyze the cell morphological features and differentiation to the osteoblastic and adipogenic lineages. Based on the in vitro and in vivo results, we identify cell volume as the true reflection of the cytoskeleton tension under stress stimuli compared with cell spreading area. By adjusting cell volume, cytoskeletal tension and cell differentiation can be regulated without affecting cell spreading area. Further study shows that the Ras-related small GTPase RAP2 inhibits the activity of mechanical transducers Lamin A/C and YAP1, playing an important role in cell volume regulation of cell differentiation. In summary, our results support the close relationship between cell volume and cytoskeleton tension. The regulatory role of cell volume on cell differentiation is modulated, at least in part, by RAP2-related mechanosensitive pathways. Our insights into how cell volume regulates cell differentiation may build a bridge between two-dimensional and three-dimensional mechanical studies in cell biology.
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