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Title:
Retinoic Acid Receptor α Knockdown Suppresses the Tumorigenicity of Esophageal Carcinoma via Wnt/β-catenin Pathway | Digestive Diseases and Sciences
Description:
Background Aberrant expression of retinoic acid receptor α (RARα) was correlated with diverse carcinomas such as acute promyelocytic leukemia and colorectal carcinoma. Nevertheless, the function and mechanism of RARα in esophageal carcinoma (EC) remain unclear. Aim To investigate the expression of RARα in EC and its effect in the tumorigenesis of EC. Methods and Results In immunohistochemistry study, RARα was overexpressed in human EC tissues, and its overexpression was closely related to the pathological differentiation, lymph node metastasis, and clinical stages in EC patients. Functionally, RARα knockdown suppressed the proliferation and metastasis of EC cells through downregulating the expression of PCNA, Ki67, MMP7, and MMP9, as well as enhanced drug susceptibility of EC cells to 5-fluorouracil and cisplatin. Mechanistically, RARα knockdown inhibited the activity of Wnt/β-catenin pathway through reducing the phosphorylation level of GSK3β at Ser-9 and inducing phosphorylation level at Tyr-216, which resulted in downregulation of its downstream targets such as MMP7, MMP9, and P-gP. Conclusions Our results demonstrated that RARα knockdown suppressed the tumorigenicity of EC via Wnt/β-catenin pathway. RARα might be a potential molecular target for EC clinical therapy.
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article, google, scholar, cancer, cas, carcinoma, acid, esophageal, retinoic, receptor, signaling, cell, pathway, rarα, chen, xiamen, wntβcatenin, zhang, kinase, knockdown, shen, china, privacy, cookies, content, data, research, expression, related, drug, squamous, resistance, publish, search, manuscript, mao, hua, liu, proliferation, cells, mmp, phosphorylation, access, nat, mol, pubmed, oncogenic, wnt, sci, biol,
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promoting β-catenin phosphorylation/degradation wnt/β-catenin signaling pathway wnt/β-catenin pathway month download article/chapter enhanced drug susceptibility gsk3β/β-catenin pathway gsk3beta/beta-catenin signaling retinoic acid receptors wnt/beta-catenin pathways dong-yan shen qing-xi chen gsk3alpha exhibits beta-catenin cerebral ischemia-induced neurogenesis nuclear receptor superfamily qian-en chen activates wnt pathway article digestive diseases lymph node metastasis reduces akt signaling retinoic acid colorectal carcinoma inhibits wif-1 expression full article pdf privacy choices/manage cookies akt/nf-kappab rarα knockdown suppressed rarα knockdown inhibited jin-xing shen tumor suppressor human ec tissues xiao-yun zhang nat rev cancer huang gl tau protein kinase article mao acute promyelocytic leukemia nat cell biol wnt signaling ethics declarations conflict drosophila shaggy kinase inhibiting p38 phosphorylation related subjects natural science foundation mol cell biol european economic area check access epithelial-mesenchymal transition living kidney donors positive feedback loop tumour biol
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headline:Retinoic Acid Receptor α Knockdown Suppresses the Tumorigenicity of Esophageal Carcinoma via Wnt/β-catenin Pathway
description:Aberrant expression of retinoic acid receptor α (RARα) was correlated with diverse carcinomas such as acute promyelocytic leukemia and colorectal carcinoma. Nevertheless, the function and mechanism of RARα in esophageal carcinoma (EC) remain unclear. To investigate the expression of RARα in EC and its effect in the tumorigenesis of EC. In immunohistochemistry study, RARα was overexpressed in human EC tissues, and its overexpression was closely related to the pathological differentiation, lymph node metastasis, and clinical stages in EC patients. Functionally, RARα knockdown suppressed the proliferation and metastasis of EC cells through downregulating the expression of PCNA, Ki67, MMP7, and MMP9, as well as enhanced drug susceptibility of EC cells to 5-fluorouracil and cisplatin. Mechanistically, RARα knockdown inhibited the activity of Wnt/β-catenin pathway through reducing the phosphorylation level of GSK3β at Ser-9 and inducing phosphorylation level at Tyr-216, which resulted in downregulation of its downstream targets such as MMP7, MMP9, and P-gP. Our results demonstrated that RARα knockdown suppressed the tumorigenicity of EC via Wnt/β-catenin pathway. RARα might be a potential molecular target for EC clinical therapy.
datePublished:2018-08-28T00:00:00Z
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keywords:
Retinoic acid receptor α
Esophageal carcinoma
Proliferation
Metastasis
Drug susceptibility
Wnt/β-catenin pathway
Gastroenterology
Hepatology
Oncology
Transplant Surgery
Biochemistry
general
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headline:Retinoic Acid Receptor α Knockdown Suppresses the Tumorigenicity of Esophageal Carcinoma via Wnt/β-catenin Pathway
description:Aberrant expression of retinoic acid receptor α (RARα) was correlated with diverse carcinomas such as acute promyelocytic leukemia and colorectal carcinoma. Nevertheless, the function and mechanism of RARα in esophageal carcinoma (EC) remain unclear. To investigate the expression of RARα in EC and its effect in the tumorigenesis of EC. In immunohistochemistry study, RARα was overexpressed in human EC tissues, and its overexpression was closely related to the pathological differentiation, lymph node metastasis, and clinical stages in EC patients. Functionally, RARα knockdown suppressed the proliferation and metastasis of EC cells through downregulating the expression of PCNA, Ki67, MMP7, and MMP9, as well as enhanced drug susceptibility of EC cells to 5-fluorouracil and cisplatin. Mechanistically, RARα knockdown inhibited the activity of Wnt/β-catenin pathway through reducing the phosphorylation level of GSK3β at Ser-9 and inducing phosphorylation level at Tyr-216, which resulted in downregulation of its downstream targets such as MMP7, MMP9, and P-gP. Our results demonstrated that RARα knockdown suppressed the tumorigenicity of EC via Wnt/β-catenin pathway. RARα might be a potential molecular target for EC clinical therapy.
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Retinoic acid receptor α
Esophageal carcinoma
Proliferation
Metastasis
Drug susceptibility
Wnt/β-catenin pathway
Gastroenterology
Hepatology
Oncology
Transplant Surgery
Biochemistry
general
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