We are analyzing https://link.springer.com/article/10.1007/s10616-024-00688-1.

Title:
Oral cell lysates reduce osteoclastogenesis in murine bone marrow cultures | Cytotechnology
Description:
Mechanical and thermal cell damage can occur due to invasive procedures related to drilling, the insertion of dental implants, and periodontal treatments. Necrotic cells release the content of their cytoplasm and membrane fragments, thereby signaling the need for repair, which includes bone resorption by osteoclasts and inflammation. Here we screened lysates from human gingival fibroblasts, HSC2 and TR146 oral squamous carcinoma cell lines, as well as murine IDG-SW3 osteocytic and RAW264.7 macrophage cell lines for their potential to modulate in vitro osteoclastogenesis in murine bone marrow cultures. We also tested the impact of necrotic lysates on modulating the expression of inflammatory cues in murine ST2 bone marrow stromal cells. We report here that independent of human or murine origin, all cell lysates significantly reduced in vitro osteoclastogenesis in bone marrow cultures, as indicated by the expression of the osteoclast marker genes cathepsin K and tartrate-resistant acid phosphatase and the respective histochemical staining in multinucleated cells. We also found that lysates from HSC2 and TR146 cells significantly pushed the expression of CCL2, CCL5, CXCL1, IL1, and IL6 in ST2 cells. These findings suggest that oral cell lysates reduce in vitro osteoclastogenesis, but only damaged oral squamous carcinoma cells can force murine stromal cells to produce an inflammatory environment.
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Keywords {🔍}

cells, lysates, pubmed, cell, article, osteoclastogenesis, bone, google, scholar, necrotic, cas, expression, ccl, oral, marrow, hsc, central, murine, prepared, data, panahipour, fibroblasts, gingival, vitro, inflammatory, reduce, tissue, stromal, research, cultures, cxcl, fig, gruber, macrophages, analysis, environment, rankl, clin, idgsw, raw, response, periodontitis, chemokine, line, trap, presence, sonication, periodontal, osteoclast, implant,

Topics {✒️}

ratio-paired t-test macrophage-inducible c-type lectin bidirectional ephrinb2-ephb4 signaling article download pdf tumor necrosis factor-alpha serum-starved necrotic cells murine idg-sw3 osteocytic platelet-rich fibrin reduce pleiotropic pro-inflammatory functions osteocytes produce interferon-beta tartrate-resistant acid phosphatase idg-sw3 osteocytic cells marrow adipocyte-derived cxcl1 tri-oval implant design regular infant formula fibroblast-rich connective tissue single-cell transcriptome analysis 20 ng/ml m-csf macrophage-colony stimulating factor lower anti-inflammatory activity methods cell lines oral epithelial cells remaining trap-positive cells multinucleated trap-positive cells murine bone marrow osteocytic cell line epithelial cell populations oral cell lysates thermal cell damage pyroptosis-mediated periodontal disease bone marrow cultures murine stromal cells lps-induced inflammatory response st2 cell line lysates significantly reduce full size image bone marrow cells idg-sw3 cells damaged oral cells sonicated living cell osteoclast marker genes necrotic cell lysates bone marrow respond riken cell bank authenticated cell cultures privacy choices/manage cookies early bone apposition necrotic cell lysate x-fold change normalized st2 cell lysates

Questions {❓}

The question remains: Why don’t DAMPs from gingival fibroblasts or those from IDG-SW3 have this activity?

Schema {🗺️}

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         headline:Oral cell lysates reduce osteoclastogenesis in murine bone marrow cultures
         description:Mechanical and thermal cell damage can occur due to invasive procedures related to drilling, the insertion of dental implants, and periodontal treatments. Necrotic cells release the content of their cytoplasm and membrane fragments, thereby signaling the need for repair, which includes bone resorption by osteoclasts and inflammation. Here we screened lysates from human gingival fibroblasts, HSC2 and TR146 oral squamous carcinoma cell lines, as well as murine IDG-SW3 osteocytic and RAW264.7 macrophage cell lines for their potential to modulate in vitro osteoclastogenesis in murine bone marrow cultures. We also tested the impact of necrotic lysates on modulating the expression of inflammatory cues in murine ST2 bone marrow stromal cells. We report here that independent of human or murine origin, all cell lysates significantly reduced in vitro osteoclastogenesis in bone marrow cultures, as indicated by the expression of the osteoclast marker genes cathepsin K and tartrate-resistant acid phosphatase and the respective histochemical staining in multinucleated cells. We also found that lysates from HSC2 and TR146 cells significantly pushed the expression of CCL2, CCL5, CXCL1, IL1, and IL6 in ST2 cells. These findings suggest that oral cell lysates reduce in vitro osteoclastogenesis, but only damaged oral squamous carcinoma cells can force murine stromal cells to produce an inflammatory environment.
         datePublished:2025-01-08T00:00:00Z
         dateModified:2025-01-08T00:00:00Z
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            Cell damage
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            Biotechnology
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      headline:Oral cell lysates reduce osteoclastogenesis in murine bone marrow cultures
      description:Mechanical and thermal cell damage can occur due to invasive procedures related to drilling, the insertion of dental implants, and periodontal treatments. Necrotic cells release the content of their cytoplasm and membrane fragments, thereby signaling the need for repair, which includes bone resorption by osteoclasts and inflammation. Here we screened lysates from human gingival fibroblasts, HSC2 and TR146 oral squamous carcinoma cell lines, as well as murine IDG-SW3 osteocytic and RAW264.7 macrophage cell lines for their potential to modulate in vitro osteoclastogenesis in murine bone marrow cultures. We also tested the impact of necrotic lysates on modulating the expression of inflammatory cues in murine ST2 bone marrow stromal cells. We report here that independent of human or murine origin, all cell lysates significantly reduced in vitro osteoclastogenesis in bone marrow cultures, as indicated by the expression of the osteoclast marker genes cathepsin K and tartrate-resistant acid phosphatase and the respective histochemical staining in multinucleated cells. We also found that lysates from HSC2 and TR146 cells significantly pushed the expression of CCL2, CCL5, CXCL1, IL1, and IL6 in ST2 cells. These findings suggest that oral cell lysates reduce in vitro osteoclastogenesis, but only damaged oral squamous carcinoma cells can force murine stromal cells to produce an inflammatory environment.
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      dateModified:2025-01-08T00:00:00Z
      pageStart:1
      pageEnd:11
      license:http://creativecommons.org/licenses/by/4.0/
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         Osteoclast
         Cell damage
         Dental implants
         Periodontal treatment
         Necrotic cell lysates
         Gingival fibroblasts
         Epithelial cells
         Macrophages
         In vitro
         Biotechnology
         Biomedicine
         general
         Biochemistry
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      address:
         name:Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland
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            address:
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               type:PostalAddress
            type:Organization
      name:Feng Shao
      affiliation:
            name:University Clinic of Dentistry, Medical University of Vienna
            address:
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               type:PostalAddress
            type:Organization
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      affiliation:
            name:University Clinic of Dentistry, Medical University of Vienna
            address:
               name:Department of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria
               type:PostalAddress
            type:Organization
            name:University of Bern
            address:
               name:Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland
               type:PostalAddress
            type:Organization
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            address:
               name:Austrian Cluster for Tissue Regeneration, Vienna, Austria
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      name:Department of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria
      name:Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland
      name:Austrian Cluster for Tissue Regeneration, Vienna, Austria

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