We are analyzing https://link.springer.com/article/10.1007/s10616-016-9952-7.

Title:
Evaluation of electroporation-induced adverse effects on adipose-derived stem cell exosomes | Cytotechnology
Description:
In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading the extracellular vesicles efficiently with the drug of choice. One of the proposed solutions to this problem has been drug loading by electroporation, where small pores are created in the membrane of the extracellular vesicles, hereby allowing for free diffusion of the drug compound into the interior of the vesicle. We investigated the utility of adipose-derived stem cells (ASCs) as an efficient exosome donor cell type with a particular focus on the treatment of glioblastoma multiforme (GBM). In addition, we evaluated electroporation-induced effects on the ASC exosomes with respect to their endogenous potential of stimulating GBM proliferation, and morphological changes to single and multiple ASC exosomes. We found that electroporation does not change the endogenous stimulatory capacity of ASC exosomes on GBM cell proliferation, but mediates adverse morphological changes including aggregation of the exosomes. In order to address this issue, we have successfully optimized the use of a trehalose-containing buffer system as a way of maintaining the structural integrity of the exosomes.
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Keywords {🔍}

article, google, scholar, exosomes, cas, cells, stem, cell, vesicles, extracellular, delivery, drug, mesenchymal, aalborg, adiposederived, johnsen, trehalose, cancer, content, electroporation, human, university, privacy, cookies, research, gudbergsson, skov, potential, vesicle, access, rna, sirna, release, biophys, molecular, dynamics, denmark, publish, search, cytotechnology, electroporationinduced, kasper, bendix, duroux, loading, glioblastoma, multiforme, gbm, asc, therapeutic,

Topics {✒️}

adipose-derived stem cells mesenchymal stem cell glioma stem cells human esc-derived mscs endothelial cell-derived exosomes stem cell research month download article/chapter unmodified tumor-derived exosomes evaluated electroporation-induced effects functional anti-mir-9 electroporation-induced adverse effects glioblastoma multiforme trehalose–lipid bilayer systems doxorubicin delivery platform targeted tumor therapy kasper bendix johnsen drug delivery purposes targeted cancer therapy donor cell type human cells breast cancer cells electrotransfected mammalian cells extracellular vesicle research tissue eng part utilizing extracellular vesicles extracellular vesicles efficiently glioma cells related subjects martin najbjerg skov gbm cell proliferation exosome-mediated delivery article cytotechnology aims full article pdf author information authors privacy choices/manage cookies mol cell biochem drug delivery cell migration trehalose improves survival molecular dynamics study check access instant access inhibit contact sensitivity emerging therapeutic opportunities nucleic acids res efficient mass producer article johnsen molecular dynamics simulations author correspondence health science

Schema {🗺️}

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         headline:Evaluation of electroporation-induced adverse effects on adipose-derived stem cell exosomes
         description:In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading the extracellular vesicles efficiently with the drug of choice. One of the proposed solutions to this problem has been drug loading by electroporation, where small pores are created in the membrane of the extracellular vesicles, hereby allowing for free diffusion of the drug compound into the interior of the vesicle. We investigated the utility of adipose-derived stem cells (ASCs) as an efficient exosome donor cell type with a particular focus on the treatment of glioblastoma multiforme (GBM). In addition, we evaluated electroporation-induced effects on the ASC exosomes with respect to their endogenous potential of stimulating GBM proliferation, and morphological changes to single and multiple ASC exosomes. We found that electroporation does not change the endogenous stimulatory capacity of ASC exosomes on GBM cell proliferation, but mediates adverse morphological changes including aggregation of the exosomes. In order to address this issue, we have successfully optimized the use of a trehalose-containing buffer system as a way of maintaining the structural integrity of the exosomes.
         datePublished:2016-02-08T00:00:00Z
         dateModified:2016-02-08T00:00:00Z
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            Drug delivery
            Adipose-derived stem cells
            Electroporation
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            Glioblastoma multiforme
            Biotechnology
            Biomedicine
            general
            Biochemistry
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      headline:Evaluation of electroporation-induced adverse effects on adipose-derived stem cell exosomes
      description:In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading the extracellular vesicles efficiently with the drug of choice. One of the proposed solutions to this problem has been drug loading by electroporation, where small pores are created in the membrane of the extracellular vesicles, hereby allowing for free diffusion of the drug compound into the interior of the vesicle. We investigated the utility of adipose-derived stem cells (ASCs) as an efficient exosome donor cell type with a particular focus on the treatment of glioblastoma multiforme (GBM). In addition, we evaluated electroporation-induced effects on the ASC exosomes with respect to their endogenous potential of stimulating GBM proliferation, and morphological changes to single and multiple ASC exosomes. We found that electroporation does not change the endogenous stimulatory capacity of ASC exosomes on GBM cell proliferation, but mediates adverse morphological changes including aggregation of the exosomes. In order to address this issue, we have successfully optimized the use of a trehalose-containing buffer system as a way of maintaining the structural integrity of the exosomes.
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         Extracellular vesicles
         Exosomes
         Drug delivery
         Adipose-derived stem cells
         Electroporation
         Trehalose
         Glioblastoma multiforme
         Biotechnology
         Biomedicine
         general
         Biochemistry
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