We are analyzing https://link.springer.com/article/10.1007/s10585-013-9585-6.

Title:
Efficacy and mechanism of action of Deguelin in suppressing metastasis of 4T1 cells | Clinical & Experimental Metastasis
Description:
Cancer related deaths in breast cancer patients are due to metastasis of the disease. Murine 4T1 cells (Murine mammary cancer cell line developed from 6-thioguanine resistant tumor) provide an excellent research tool for metastasis related studies because these cells are highly aggressive and readily metastasize to the lungs. In this study we determined the effect of Deguelin on in vivo/vitro growth and metastasis of 4T1 cells. Deguelin inhibited the in vitro growth of 4T1 cells in a time and dose dependent manner accompanied with reduced nuclear PCNA immunostaining. In cells treated with Deguelin, reduced expression of nuclear c-Met, and its downstream targets such p-ERK and p-AKT was observed. Deguelin reduced the cell migration in 4T1 cells as determined by scratch wound assay. Combined treatment with Deguelin + ERK or PI3K/AKT inhibitor had no additional effect on cell migration. These results indicated that the action of Deguelin on cell migration may be mediated by AKT and ERK mediated signaling pathways. In vivo, Deguelin treatment significantly inhibited growth of 4T1 cells. Deguelin also reduced the occurrence of metastatic lung lesions by 33 % when cells were injected intravenously into Balb/c female mice. There was no difference in the body weight, nor was there a difference in liver and spleen weights between vehicle treated-control and Deguelin-treated animals, which indicated that Deguelin was nontoxic at the dose used in the present study. These results provide rationale for developing Deguelin as a chemotherapeutic agent for triple negative breast cancer patients.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Health & Fitness

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don't see any clear sign of profit-making.

Not all websites focus on profit; some are designed to educate, connect people, or share useful tools. People create websites for numerous reasons. And this could be one such example. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

cancer, google, scholar, article, pubmed, cas, deguelin, cells, growth, factor, breast, cell, human, metastasis, receptor, lee, expression, mehta, res, lung, hepatocyte, protein, pathway, negative, apoptosis, chen, research, tumor, cmet, inhibitor, kinase, int, clin, supplementary, material, privacy, cookies, content, migration, erk, carcinoma, met, pezzuto, hong, author, publish, search, efficacy, action, patel,

Topics {✒️}

nuclear factor kappa-light-chain-enhancer month download article/chapter mouse strain ak-thymoma small-cell lung carcinomas phosphatidylinositol 3-kinase/akt pathway pyrene-induced lung tumorigenesis akash gupta di renzo mf author information authors rutulkumar patel hepatocyte growth factor induce c-met expression murine 4t1 cells human breast carcinoma c-met proto-oncogene mammary epithelial cells igf-binding protein-3 expression breast cancer patients human breast cancer full article pdf breast cancer cells nuclear c-met author correspondence wnt signaling pathway metastasis related studies human pancreatic cancer iit research institute erk protein kinase hgf/sf receptor excellent research tool privacy choices/manage cookies triple negative status metastatic lung lesions estrogen receptor negative deguelin inhibits growth lung cancer cells nf-kappab pathway 6-thioguanine resistant tumor lung tumor model akt/mtor pathway progesterone receptor negative deguelin-induced inhibition tnbc papillary thyroid carcinoma hgf-induced proliferation c-met participate hypoxia-inducible factor hypoxia inducible factor-1 met receptor expression deguelin targeting

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Efficacy and mechanism of action of Deguelin in suppressing metastasis of 4T1 cells
         description:Cancer related deaths in breast cancer patients are due to metastasis of the disease. Murine 4T1 cells (Murine mammary cancer cell line developed from 6-thioguanine resistant tumor) provide an excellent research tool for metastasis related studies because these cells are highly aggressive and readily metastasize to the lungs. In this study we determined the effect of Deguelin on in vivo/vitro growth and metastasis of 4T1 cells. Deguelin inhibited the in vitro growth of 4T1 cells in a time and dose dependent manner accompanied with reduced nuclear PCNA immunostaining. In cells treated with Deguelin, reduced expression of nuclear c-Met, and its downstream targets such p-ERK and p-AKT was observed. Deguelin reduced the cell migration in 4T1 cells as determined by scratch wound assay. Combined treatment with Deguelin + ERK or PI3K/AKT inhibitor had no additional effect on cell migration. These results indicated that the action of Deguelin on cell migration may be mediated by AKT and ERK mediated signaling pathways. In vivo, Deguelin treatment significantly inhibited growth of 4T1 cells. Deguelin also reduced the occurrence of metastatic lung lesions by 33 % when cells were injected intravenously into Balb/c female mice. There was no difference in the body weight, nor was there a difference in liver and spleen weights between vehicle treated-control and Deguelin-treated animals, which indicated that Deguelin was nontoxic at the dose used in the present study. These results provide rationale for developing Deguelin as a chemotherapeutic agent for triple negative breast cancer patients.
         datePublished:2013-05-05T00:00:00Z
         dateModified:2013-05-05T00:00:00Z
         pageStart:855
         pageEnd:866
         sameAs:https://doi.org/10.1007/s10585-013-9585-6
         keywords:
            4T1 cells
            Metastasis
            Biomarkers
            Deguelin
            TNBC
            Cancer Research
            Biomedicine
            general
            Oncology
            Hematology
            Surgical Oncology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-013-9585-6/MediaObjects/10585_2013_9585_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-013-9585-6/MediaObjects/10585_2013_9585_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-013-9585-6/MediaObjects/10585_2013_9585_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-013-9585-6/MediaObjects/10585_2013_9585_Fig4_HTML.gif
         isPartOf:
            name:Clinical & Experimental Metastasis
            issn:
               1573-7276
               0262-0898
            volumeNumber:30
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Netherlands
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Rajeshwari R. Mehta
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Harshadadevi Katta
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Amit Kalra
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Rutulkumar Patel
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Akash Gupta
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Fatouma Alimirah
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Genoveva Murillo
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xinjian Peng
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Aditya Unni
               affiliation:
                     name:Illinois Institute of Technology
                     address:
                        name:Department of Chemistry, Illinois Institute of Technology, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Miguel Muzzio
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Rajendra G. Mehta
               affiliation:
                     name:IIT Research Institute
                     address:
                        name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Efficacy and mechanism of action of Deguelin in suppressing metastasis of 4T1 cells
      description:Cancer related deaths in breast cancer patients are due to metastasis of the disease. Murine 4T1 cells (Murine mammary cancer cell line developed from 6-thioguanine resistant tumor) provide an excellent research tool for metastasis related studies because these cells are highly aggressive and readily metastasize to the lungs. In this study we determined the effect of Deguelin on in vivo/vitro growth and metastasis of 4T1 cells. Deguelin inhibited the in vitro growth of 4T1 cells in a time and dose dependent manner accompanied with reduced nuclear PCNA immunostaining. In cells treated with Deguelin, reduced expression of nuclear c-Met, and its downstream targets such p-ERK and p-AKT was observed. Deguelin reduced the cell migration in 4T1 cells as determined by scratch wound assay. Combined treatment with Deguelin + ERK or PI3K/AKT inhibitor had no additional effect on cell migration. These results indicated that the action of Deguelin on cell migration may be mediated by AKT and ERK mediated signaling pathways. In vivo, Deguelin treatment significantly inhibited growth of 4T1 cells. Deguelin also reduced the occurrence of metastatic lung lesions by 33 % when cells were injected intravenously into Balb/c female mice. There was no difference in the body weight, nor was there a difference in liver and spleen weights between vehicle treated-control and Deguelin-treated animals, which indicated that Deguelin was nontoxic at the dose used in the present study. These results provide rationale for developing Deguelin as a chemotherapeutic agent for triple negative breast cancer patients.
      datePublished:2013-05-05T00:00:00Z
      dateModified:2013-05-05T00:00:00Z
      pageStart:855
      pageEnd:866
      sameAs:https://doi.org/10.1007/s10585-013-9585-6
      keywords:
         4T1 cells
         Metastasis
         Biomarkers
         Deguelin
         TNBC
         Cancer Research
         Biomedicine
         general
         Oncology
         Hematology
         Surgical Oncology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-013-9585-6/MediaObjects/10585_2013_9585_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-013-9585-6/MediaObjects/10585_2013_9585_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-013-9585-6/MediaObjects/10585_2013_9585_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-013-9585-6/MediaObjects/10585_2013_9585_Fig4_HTML.gif
      isPartOf:
         name:Clinical & Experimental Metastasis
         issn:
            1573-7276
            0262-0898
         volumeNumber:30
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Netherlands
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Rajeshwari R. Mehta
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Harshadadevi Katta
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Amit Kalra
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Rutulkumar Patel
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Akash Gupta
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Fatouma Alimirah
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Genoveva Murillo
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xinjian Peng
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Aditya Unni
            affiliation:
                  name:Illinois Institute of Technology
                  address:
                     name:Department of Chemistry, Illinois Institute of Technology, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Miguel Muzzio
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Rajendra G. Mehta
            affiliation:
                  name:IIT Research Institute
                  address:
                     name:Cancer Biology Division, IIT Research Institute, Chicago, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Clinical & Experimental Metastasis
      issn:
         1573-7276
         0262-0898
      volumeNumber:30
Organization:
      name:Springer Netherlands
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:Illinois Institute of Technology
      address:
         name:Department of Chemistry, Illinois Institute of Technology, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
      name:IIT Research Institute
      address:
         name:Cancer Biology Division, IIT Research Institute, Chicago, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Rajeshwari R. Mehta
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Harshadadevi Katta
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Amit Kalra
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Rutulkumar Patel
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Akash Gupta
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Fatouma Alimirah
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Genoveva Murillo
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Xinjian Peng
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Aditya Unni
      affiliation:
            name:Illinois Institute of Technology
            address:
               name:Department of Chemistry, Illinois Institute of Technology, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Miguel Muzzio
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      name:Rajendra G. Mehta
      affiliation:
            name:IIT Research Institute
            address:
               name:Cancer Biology Division, IIT Research Institute, Chicago, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Department of Chemistry, Illinois Institute of Technology, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
      name:Cancer Biology Division, IIT Research Institute, Chicago, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(163)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.24s.

LINK . SPRINGER . COM {}