We are analyzing https://link.springer.com/article/10.1007/s10585-011-9415-7.

Title:
Reduction of metastasis using a non-volatile buffer | Clinical & Experimental Metastasis
Description:
The tumor microenvironment is acidic as a consequence of upregulated glycolysis and poor perfusion and this acidity, in turn, promotes invasion and metastasis. We have recently demonstrated that chronic consumption of sodium bicarbonate increased tumor pH and reduced spontaneous and experimental metastases. This occurred without affecting systemic pH, which was compensated. Additionally, these prior data did not rule out the possibility that bicarbonate was working though effects on carbonic anhydrase, and not as a buffer per se. Here, we present evidence that chronic ingestion of a non-volatile buffer, 2-imidazole-1-yl-3-ethoxycarbonylpropionic acid (IEPA) with a pK a of 6.9 also reduced metastasis in an experimental PC3M prostate cancer mouse model. Animals (n = 30) were injected with luciferase expressing PC3M prostate cancer cells either subcutaneously (s.c., n = 10) or intravenously (i.v., n = 20). Four days prior to inoculations, half of the animals for each experiment were provided drinking water containing 200 mM IEPA buffer. Animals were imaged weekly to follow metastasis, and these data showed that animals treated with IEPA had significantly fewer experimental lung metastasis compared to control groups (P < 0.04). Consistent with prior work, the pH of treated tumors was elevated compared to controls. IEPA is observable by in vivo magnetic resonance spectroscopy and this was used to measure the presence of IEPA in the bladder, confirming that it was orally available. The results of this study indicate that metastasis can be reduced by non-volatile buffers as well as bicarbonate and thus the effect appears to be due to pH buffering per se.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Health & Fitness
Science
Education

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't tell how the site generates income.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

iepa, tumor, cancer, metastasis, article, metastases, google, scholar, animals, cells, cas, pubmed, experimental, primary, buffer, imaging, bicarbonate, effect, tumors, tap, fig, images, animal, data, water, magnetic, resonance, obtained, nonvolatile, spontaneous, pcm, buffers, injection, mice, prostate, vivo, growth, model, study, extracellular, shown, bioluminescence, mouse, acidic, invasion, prior, days, work, cell, models,

Topics {✒️}

2-imidazole-1-yl-3-ethoxycarbonyl-propionic acid 2-imidazole-1-yl-3-ethoxycarbonylpropionic acid article download pdf basler tri-linear-array related subjects microenvironment-induced cancer metastasis minimum essential media/earle higher steady-state concentrations arig ibrahim hashim membrane type-matrix metalloproteinases luciferase-expressing cells magnetic resonance spectroscopy fast spin echo breast cancer metastasis prostate cancer metastasis tailed students t-test cancer imaging research magnetic resonance imaging privacy choices/manage cookies pc3m prostate model full access �acid-mediated invasion hypothesis mem/ebss media t2 weighted images reaction-diffusion models analytic microscopy research reduce lung metastasis varian pulse sequence extra cellular space synthetic imidazole buffer significant inhibitory effect favorable clinical outcomes note log scale mathematical models supported cancer cells acidic prostate animal models increased experimental metastases vivo spectroscopic imaging lung imaging sessions summary significant reduction glucose metabolism combined varian vnmrj software promote immune infiltration pathogen free rooms resulting dose 150 μg/ light-tight chamber 8na objective lens intensity specification defined flip angle 90 deg synthetic imadizolic base

Questions {❓}

Griffiths JR (1991) Are cancer cells acidic?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Reduction of metastasis using a non-volatile buffer
         description:The tumor microenvironment is acidic as a consequence of upregulated glycolysis and poor perfusion and this acidity, in turn, promotes invasion and metastasis. We have recently demonstrated that chronic consumption of sodium bicarbonate increased tumor pH and reduced spontaneous and experimental metastases. This occurred without affecting systemic pH, which was compensated. Additionally, these prior data did not rule out the possibility that bicarbonate was working though effects on carbonic anhydrase, and not as a buffer per se. Here, we present evidence that chronic ingestion of a non-volatile buffer, 2-imidazole-1-yl-3-ethoxycarbonylpropionic acid (IEPA) with a pK a of 6.9 also reduced metastasis in an experimental PC3M prostate cancer mouse model. Animals (n = 30) were injected with luciferase expressing PC3M prostate cancer cells either subcutaneously (s.c., n = 10) or intravenously (i.v., n = 20). Four days prior to inoculations, half of the animals for each experiment were provided drinking water containing 200 mM IEPA buffer. Animals were imaged weekly to follow metastasis, and these data showed that animals treated with IEPA had significantly fewer experimental lung metastasis compared to control groups (P < 0.04). Consistent with prior work, the pH of treated tumors was elevated compared to controls. IEPA is observable by in vivo magnetic resonance spectroscopy and this was used to measure the presence of IEPA in the bladder, confirming that it was orally available. The results of this study indicate that metastasis can be reduced by non-volatile buffers as well as bicarbonate and thus the effect appears to be due to pH buffering per se.
         datePublished:2011-08-23T00:00:00Z
         dateModified:2011-08-23T00:00:00Z
         pageStart:841
         pageEnd:849
         license:https://creativecommons.org/licenses/by-nc/2.0
         sameAs:https://doi.org/10.1007/s10585-011-9415-7
         keywords:
            Reduction of metastasis
            Buffering
            pH
            Acidic microenvironment
            PC3M
            Prostate cancer
            Cancer Research
            Biomedicine
            general
            Oncology
            Hematology
            Surgical Oncology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig5_HTML.gif
         isPartOf:
            name:Clinical & Experimental Metastasis
            issn:
               1573-7276
               0262-0898
            volumeNumber:28
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Netherlands
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Arig Ibrahim Hashim
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Heather H. Cornnell
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Maria de Lourdes Coelho Ribeiro
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Dominique Abrahams
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jessica Cunningham
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Mark Lloyd
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Analytic Microscopy Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Gary V. Martinez
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Robert A. Gatenby
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Robert J. Gillies
               affiliation:
                     name:H. Lee Moffitt Cancer Center and Research Institute
                     address:
                        name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Reduction of metastasis using a non-volatile buffer
      description:The tumor microenvironment is acidic as a consequence of upregulated glycolysis and poor perfusion and this acidity, in turn, promotes invasion and metastasis. We have recently demonstrated that chronic consumption of sodium bicarbonate increased tumor pH and reduced spontaneous and experimental metastases. This occurred without affecting systemic pH, which was compensated. Additionally, these prior data did not rule out the possibility that bicarbonate was working though effects on carbonic anhydrase, and not as a buffer per se. Here, we present evidence that chronic ingestion of a non-volatile buffer, 2-imidazole-1-yl-3-ethoxycarbonylpropionic acid (IEPA) with a pK a of 6.9 also reduced metastasis in an experimental PC3M prostate cancer mouse model. Animals (n = 30) were injected with luciferase expressing PC3M prostate cancer cells either subcutaneously (s.c., n = 10) or intravenously (i.v., n = 20). Four days prior to inoculations, half of the animals for each experiment were provided drinking water containing 200 mM IEPA buffer. Animals were imaged weekly to follow metastasis, and these data showed that animals treated with IEPA had significantly fewer experimental lung metastasis compared to control groups (P < 0.04). Consistent with prior work, the pH of treated tumors was elevated compared to controls. IEPA is observable by in vivo magnetic resonance spectroscopy and this was used to measure the presence of IEPA in the bladder, confirming that it was orally available. The results of this study indicate that metastasis can be reduced by non-volatile buffers as well as bicarbonate and thus the effect appears to be due to pH buffering per se.
      datePublished:2011-08-23T00:00:00Z
      dateModified:2011-08-23T00:00:00Z
      pageStart:841
      pageEnd:849
      license:https://creativecommons.org/licenses/by-nc/2.0
      sameAs:https://doi.org/10.1007/s10585-011-9415-7
      keywords:
         Reduction of metastasis
         Buffering
         pH
         Acidic microenvironment
         PC3M
         Prostate cancer
         Cancer Research
         Biomedicine
         general
         Oncology
         Hematology
         Surgical Oncology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10585-011-9415-7/MediaObjects/10585_2011_9415_Fig5_HTML.gif
      isPartOf:
         name:Clinical & Experimental Metastasis
         issn:
            1573-7276
            0262-0898
         volumeNumber:28
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Netherlands
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Arig Ibrahim Hashim
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Heather H. Cornnell
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Maria de Lourdes Coelho Ribeiro
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Dominique Abrahams
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jessica Cunningham
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mark Lloyd
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Analytic Microscopy Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gary V. Martinez
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Robert A. Gatenby
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Robert J. Gillies
            affiliation:
                  name:H. Lee Moffitt Cancer Center and Research Institute
                  address:
                     name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Clinical & Experimental Metastasis
      issn:
         1573-7276
         0262-0898
      volumeNumber:28
Organization:
      name:Springer Netherlands
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Analytic Microscopy Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
      name:H. Lee Moffitt Cancer Center and Research Institute
      address:
         name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Arig Ibrahim Hashim
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Heather H. Cornnell
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
      name:Maria de Lourdes Coelho Ribeiro
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
      name:Dominique Abrahams
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
      name:Jessica Cunningham
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
      name:Mark Lloyd
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Analytic Microscopy Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
      name:Gary V. Martinez
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
      name:Robert A. Gatenby
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
      name:Robert J. Gillies
      affiliation:
            name:H. Lee Moffitt Cancer Center and Research Institute
            address:
               name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
      name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
      name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
      name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
      name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
      name:Department of Analytic Microscopy Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
      name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
      name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA
      name:Department of Cancer Imaging Research, H. Lee Moffitt Cancer Center and Research Institute, Tampa, USA

External Links {🔗}(118)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.54s.

LINK . SPRINGER . COM {}