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widespread intra-species cross-contamination dna microsatellite analysis lcc15-mb cell line mda-mb-435-lcc15 cells original lcc15-mb cells mda-mb-435 cell line month download article/chapter multidrug-resistant cell line delity ofmda-mb-435 cells mda-mb-435-lcc cells chromosomal number human breast-bone metastasis mda435/lcc6 andmda435/lcc6mdr1 'spontane-ously' transformed sublines lcc15-mb cells chromosome number locus-speci cminisatellite probe false cell lines mda-mb-435 cells prostate cell lines lcc15-mb stocks human breast cancer heteroploid cell lines mda-mb-435-lcc human breast cancercells human breast milk dna sequencecopy number cell cross-contamination full article pdf cell line designationchange ma-160 cell line comparative genomic hybridisation parative genomic hybridization cell line characterization privacy choices/manage cookies depictcancer cell invasiveness lcc15-mb marker chromosomes matrigel-based misidentification related subjects 500 cell lines cell lines lombardi cancer center original karyotype reported check access instant access breast tumor cancer research previously published studies

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         headline:LCC15-MB Cells are MDA-MB-435: A Review of Misidentified Breast and prostate cell lines
         description:Current stocks of the LCC15-MB cell line, which we originally isolated from a human breast-bone metastasis, were found to be genetically matched to the MDA-MB-435 cell line from the Lombardi Cancer Center (MDA-MB-435-LCC) using comparative genomic hybridisation, DNA microsatellite analysis and chromosomal number. LCC15-MB stocks used for our previously published studies as well as the earliest available LCC15-MB cells also showed identity to MDA-MB-435-LCC cells. The original karyotype reported for LCC15-MB cells was considerably different to that of MDA-MB-435 cells, indicating that the original LCC15-MB cells were lost to contamination by MDA-MB-435-LCC cells. Chromosome number is the simplest test to distinguish original LCC15-MB cells (n ∼ 75) from MDA-MB-435 (n ∼ 52). Collectively, our results prove that LCC15-MB cells currently available are MDA-MB-435 cells and we suggest their re-designation as MDA-MB-435-LCC15 cells. We also review the known misclassification of breast and prostate cancer cell lines to date and have initiated a register maintained at http//www.svi.edu.au/cell_lines_registry.doc.
         datePublished:
         dateModified:
         pageStart:535
         pageEnd:541
         sameAs:https://doi.org/10.1007/s10585-004-4117-z
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            cell lines
            chromosomal number
            LCC15-MB
            MDA-MB-435
            microsatellite analysis
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            Cancer Research
            Biomedicine
            general
            Oncology
            Hematology
            Surgical Oncology
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      headline:LCC15-MB Cells are MDA-MB-435: A Review of Misidentified Breast and prostate cell lines
      description:Current stocks of the LCC15-MB cell line, which we originally isolated from a human breast-bone metastasis, were found to be genetically matched to the MDA-MB-435 cell line from the Lombardi Cancer Center (MDA-MB-435-LCC) using comparative genomic hybridisation, DNA microsatellite analysis and chromosomal number. LCC15-MB stocks used for our previously published studies as well as the earliest available LCC15-MB cells also showed identity to MDA-MB-435-LCC cells. The original karyotype reported for LCC15-MB cells was considerably different to that of MDA-MB-435 cells, indicating that the original LCC15-MB cells were lost to contamination by MDA-MB-435-LCC cells. Chromosome number is the simplest test to distinguish original LCC15-MB cells (n ∼ 75) from MDA-MB-435 (n ∼ 52). Collectively, our results prove that LCC15-MB cells currently available are MDA-MB-435 cells and we suggest their re-designation as MDA-MB-435-LCC15 cells. We also review the known misclassification of breast and prostate cancer cell lines to date and have initiated a register maintained at http//www.svi.edu.au/cell_lines_registry.doc.
      datePublished:
      dateModified:
      pageStart:535
      pageEnd:541
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         cell lines
         chromosomal number
         LCC15-MB
         MDA-MB-435
         microsatellite analysis
         misidentification
         Cancer Research
         Biomedicine
         general
         Oncology
         Hematology
         Surgical Oncology
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                     name:Victorian Breast Cancer Research Consortium Groups for Invasion and Metastasis of Breast Cancer, St. Vincent's Institute of Medical Research, USA
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