We are analyzing https://link.springer.com/article/10.1007/s10549-008-0174-3.

Title:
Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients | Breast Cancer Research and Treatment
Description:
Purpose Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC’s) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC’s. Methods To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.
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Keywords {🔍}

cancer, breast, google, scholar, pubmed, article, cas, cells, patients, detection, bone, marrow, clin, rtpcr, tumor, blood, oncol, circulating, disseminated, chain, reaction, realtime, res, polymerase, peripheral, clinical, prognostic, van, comparison, access, privacy, cookies, content, research, evaluation, epithelial, becker, solomayer, correlation, primary, reverse, int, quantitative, data, publish, search, based, routine, study, beckerpergola,

Topics {✒️}

real-time rt-pcr protocols real-time rt-pcr correlates real-time rt-pcr detection christina schuetz & tanja fehm month download article/chapter ck-19 mrna-positive cells high-risk breast cancer reverse transcription-pcr disseminated tumor cells cytokeratin 19 rt-pcr study tumor micrometastasis real-time quantification immunobead rt-pcr rt-pcr amplification full article pdf disseminated epithelial cells disseminated tumour cells cytokeratin-positive cells circulating tumor cells privacy choices/manage cookies assays based isolated tumor cells ck20 rt-pcr occult breast cancer occult metastatic cells micrometastatic breast cancer isolated micrometastatic cells immunomagnetic cell sorting circulating tumour cells circulating melanoma cells circulating epithelial cells bone marrow micrometastasis bone marrow aspirates early breast cancer iii breast cancer suspected breast cancer operable breast cancer metastatic breast cancer tumor cell detection related subjects diel ij negative immunomagnetic separation primary breast cancer bone marrow biopsy routine clinical laboratory blood/bone marrow breast cancer patients rt-pcr primary colorectal cancer european economic area

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Are disseminated tumor cells in bone marrow and tumor-stroma ratio clinically applicable for patients undergoing surgical resection of primary colorectal cancer?

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         headline:Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients
         description: Purpose Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC’s) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC’s. Methods To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.
         datePublished:2008-09-14T00:00:00Z
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            Disseminated tumor cells
            Immunocytochemistry
            Minimal residual disease
            Bone marrow
            Breast cancer
            RT-PCR
            Oncology
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      headline:Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients
      description: Purpose Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC’s) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC’s. Methods To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.
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         Immunocytochemistry
         Minimal residual disease
         Bone marrow
         Breast cancer
         RT-PCR
         Oncology
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