We are analyzing https://link.springer.com/article/10.1007/s10529-014-1532-3.

Title:
The role of malic enzyme as the provider of NADPH in oleaginous microorganisms: a reappraisal and unsolved problems | Biotechnology Letters
Description:
Malic enzyme (ME; NADP+-dependent; EC 1.1.40) provides NADPH for lipid biosynthesis in oleaginous microorganisms. Its role in vivo depends on there being an adequate supply of NADH to drive malate dehydrogenase to convert oxaloacetate to malate as a component of a cycle of three reactions: pyruvate → oxaloacetate → malate and, by the action of ME, back to pyruvate. However, the availability of cytosolic NADH is limited and, consequently, ancillary means of producing NADPH are necessary. Stoichiometries are given for the conversion of glucose to triacylglycerols involving ME with and without the reactions of the pentose phosphate pathway (PPP) as an additional source of NADPH. Some oleaginous microorganisms (such as Yarrowia lipolytica), however, lack a cytosolic ME and, if the PPP is the sole provider of NADPH, the theoretical yield of triacylglycerol from glucose falls to 27.6 % (w/w) from 31.6 % when ME is present. An alternative route for NADPH generation via a cytosolic isocitrate dehydrogenase (NADP+-dependent) is then discussed.
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Keywords {🔍}

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Topics {✒️}

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Questions {❓}

Jack of all trades or master of few?
Zhang Y, Adams IP, Ratledge C (2007) Malic enzyme: the controlling activity for lipid production?

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            Microbiology
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      headline:The role of malic enzyme as the provider of NADPH in oleaginous microorganisms: a reappraisal and unsolved problems
      description:Malic enzyme (ME; NADP+-dependent; EC 1.1.40) provides NADPH for lipid biosynthesis in oleaginous microorganisms. Its role in vivo depends on there being an adequate supply of NADH to drive malate dehydrogenase to convert oxaloacetate to malate as a component of a cycle of three reactions: pyruvate → oxaloacetate → malate and, by the action of ME, back to pyruvate. However, the availability of cytosolic NADH is limited and, consequently, ancillary means of producing NADPH are necessary. Stoichiometries are given for the conversion of glucose to triacylglycerols involving ME with and without the reactions of the pentose phosphate pathway (PPP) as an additional source of NADPH. Some oleaginous microorganisms (such as Yarrowia lipolytica), however, lack a cytosolic ME and, if the PPP is the sole provider of NADPH, the theoretical yield of triacylglycerol from glucose falls to 27.6 % (w/w) from 31.6 % when ME is present. An alternative route for NADPH generation via a cytosolic isocitrate dehydrogenase (NADP+-dependent) is then discussed.
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         Fatty acid biosynthesis
         Isocitrate dehydrogenase
         Lipid accumulation
         Malic enzyme
         Pentose phosphate pathway
         Stoichiometries
         Microbiology
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         Applied Microbiology
         Biochemistry
         general
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