We are analyzing https://link.springer.com/article/10.1007/s10142-022-00929-6.

Title:
Comparison of genotyping by sequencing procedures to determine population genetic structure | Functional & Integrative Genomics
Description:
Advancements in technology over the past few decades have resulted in the development of genome sequencing at lower costs. Protocols, costs, and the amount of data produced by different sequencing technologies are highly variable. Ion Torrent and Illumina sequencing instruments are two sequencing technologies which use very similar library preparation procedures. Enzymatic combinations can be changed in genotyping by sequencing (GbS) library protocols without significant adjustments. To compare the outputs from two different GbS procedures, we sequenced samples of two sister species of yellow-nosed albatross collected at multiple geographic locations. The data sets involving different sequencing instruments and enzymatic combinations were analysed using the Stacks pipeline and aligned to the same reference genome. Both procedures identified the same genetic clusters separating Atlantic and Indian yellow-nosed albatross and substructure within Indian yellow-nosed albatross.
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Keywords {🔍}

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Topics {✒️}

month download article/chapter ion torrent platform yellow-nosed albatross collected indian yellow-nosed albatross sam/bam format v1 sequence alignment/map format high-density genetic maps high-throughput sequencing reads ion torrent illumina sequencing instruments cold spring harbor generation sequencing technology full article pdf engineering research council privacy choices/manage cookies pcr-based techniques enzyme genotyping de novo assemblies combining population genomics variant call format generation sequencing technologies exome library construction generation population genetics holds exclusive rights high-density genotyping data sets involving european economic area multiple geographic locations related subjects rapid salt-extraction pose conservation concerns burrows-wheeler transform chain-terminating inhibitors bmc genomics 20 conditions privacy policy analysis tool set accepted manuscript version abeyrama & theresa accepting optional cookies double digest radseq additional information publisher' dna sequencing article log published maps article number 9 genetic markers author information authors population genomics journal finder publish article cite

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         headline:Comparison of genotyping by sequencing procedures to determine population genetic structure
         description:Advancements in technology over the past few decades have resulted in the development of genome sequencing at lower costs. Protocols, costs, and the amount of data produced by different sequencing technologies are highly variable. Ion Torrent and Illumina sequencing instruments are two sequencing technologies which use very similar library preparation procedures. Enzymatic combinations can be changed in genotyping by sequencing (GbS) library protocols without significant adjustments. To compare the outputs from two different GbS procedures, we sequenced samples of two sister species of yellow-nosed albatross collected at multiple geographic locations. The data sets involving different sequencing instruments and enzymatic combinations were analysed using the Stacks pipeline and aligned to the same reference genome. Both procedures identified the same genetic clusters separating Atlantic and Indian yellow-nosed albatross and substructure within Indian yellow-nosed albatross.
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            Single-end
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            Microbial Genetics and Genomics
            Biochemistry
            general
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            Animal Genetics and Genomics
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      headline:Comparison of genotyping by sequencing procedures to determine population genetic structure
      description:Advancements in technology over the past few decades have resulted in the development of genome sequencing at lower costs. Protocols, costs, and the amount of data produced by different sequencing technologies are highly variable. Ion Torrent and Illumina sequencing instruments are two sequencing technologies which use very similar library preparation procedures. Enzymatic combinations can be changed in genotyping by sequencing (GbS) library protocols without significant adjustments. To compare the outputs from two different GbS procedures, we sequenced samples of two sister species of yellow-nosed albatross collected at multiple geographic locations. The data sets involving different sequencing instruments and enzymatic combinations were analysed using the Stacks pipeline and aligned to the same reference genome. Both procedures identified the same genetic clusters separating Atlantic and Indian yellow-nosed albatross and substructure within Indian yellow-nosed albatross.
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         Illumina NovaSeq
         Restriction enzyme
         Paired-end
         Single-end
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         Plant Genetics and Genomics
         Microbial Genetics and Genomics
         Biochemistry
         general
         Bioinformatics
         Animal Genetics and Genomics
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