We are analyzing https://link.springer.com/article/10.1007/s00441-024-03934-2.

Title:
CRISPR-based genetic screens in human pluripotent stem cells derived neurons and brain organoids | Cell and Tissue Research
Description:
Recent large-scale genome-wide association and single-cell RNA sequencing (scRNA-seq) studies have uncovered disease-associated genetic risk factors and cell type-specific genetic alterations. However, our understanding of how these genetic variants cause diseases and the underlying mechanisms remains largely unknown. Functional genomics screens using CRISPR-based technologies offer an effective tool for studying genes relevant to disease phenotypes. Here, we summarize recent CRISPR-based functional genomics screen approaches applied to human pluripotent stem cell (hPSC)-derived neurons and brain organoids. These screens have identified genes crucial for neurogenesis, neuronal survival, morphological development, and migration. Combining CRISPR-based genetic screens with scRNA-seq, researchers have revealed downstream genes and cellular pathways impacted by these genetic variants in human neural cells, providing new insights into the pathogenesis of neurodevelopmental disorders, such as microcephaly and autism spectrum disorders. Finally, we discuss current challenges and future directions for using CRISPR-based screens in furthering our understanding of neurological diseases and developing potential therapeutic strategies. Despite challenges, CRISPR-based screens have enormous potential for advancing the therapeutic development of many diseases.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Business & Finance

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don't see any clear sign of profit-making.

Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Link.springer.com might be making money, but it's not detectable how they're doing it.

Keywords {🔍}

pubmed, article, google, scholar, cas, central, cell, screens, human, crispr, genetic, cells, crisprbased, neurons, crisprcas, chen, nature, genomewide, functional, genome, editing, stem, screening, research, brain, zhao, genomics, screen, nat, wang, sci, guo, diseases, disease, neurological, usa, privacy, cookies, content, information, pluripotent, organoids, xinyu, singlecell, genes, access, science, identifies, author, springer,

Topics {✒️}

genome-scale crispr-mediated control crispr-based functional genomics c9orf72 dipeptide-repeat-protein toxicity genome-wide crispr screen genome-wide crispr screens month download article/chapter human ipsc-derived neurons crispr-based genetic screens crispr-cas9 screens identify human neural cells crispr-based epigenome editing investigate neurological diseases crispr-based gene editing crispr interference-based platform crispr-based technologies offer functional genomics screens genome-wide crispri/ linking crispr-pooled screens single-cell rna sequencing pooled crispr screening single-cell transcriptome readout crispr/cas9 screen kellett mid-career award crispr/cas system crispr-cas nucleases single-cell rna-seq double-stranded dna cleavage xinyu zhao crispr genetic screens crispr-cas9 screens elucidating disease-relevant states genome editing strategies pooled genetic screens crispr-based screens full article pdf replace genome editing genetic risk factors cellular pathways impacted article guo multimodal genetic screens tissue research aims genome engineering neurological diseases privacy choices/manage cookies eagle autism foundation traumatic brain injury optical pooled screens synthetic crispr reagents crispr activator efficacy single cells

Schema {🗺️}

WebPage:
      mainEntity:
         headline:CRISPR-based genetic screens in human pluripotent stem cells derived neurons and brain organoids
         description:Recent large-scale genome-wide association and single-cell RNA sequencing (scRNA-seq) studies have uncovered disease-associated genetic risk factors and cell type-specific genetic alterations. However, our understanding of how these genetic variants cause diseases and the underlying mechanisms remains largely unknown. Functional genomics screens using CRISPR-based technologies offer an effective tool for studying genes relevant to disease phenotypes. Here, we summarize recent CRISPR-based functional genomics screen approaches applied to human pluripotent stem cell (hPSC)-derived neurons and brain organoids. These screens have identified genes crucial for neurogenesis, neuronal survival, morphological development, and migration. Combining CRISPR-based genetic screens with scRNA-seq, researchers have revealed downstream genes and cellular pathways impacted by these genetic variants in human neural cells, providing new insights into the pathogenesis of neurodevelopmental disorders, such as microcephaly and autism spectrum disorders. Finally, we discuss current challenges and future directions for using CRISPR-based screens in furthering our understanding of neurological diseases and developing potential therapeutic strategies. Despite challenges, CRISPR-based screens have enormous potential for advancing the therapeutic development of many diseases.
         datePublished:2024-11-25T00:00:00Z
         dateModified:2024-11-25T00:00:00Z
         pageStart:1
         pageEnd:8
         sameAs:https://doi.org/10.1007/s00441-024-03934-2
         keywords:
            Genetic screens
            CRISPR
            Neurological diseases
            Neurons
            Organoids
            Human Genetics
            Proteomics
            Molecular Medicine
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-024-03934-2/MediaObjects/441_2024_3934_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-024-03934-2/MediaObjects/441_2024_3934_Fig2_HTML.jpg
         isPartOf:
            name:Cell and Tissue Research
            issn:
               1432-0878
               0302-766X
            volumeNumber:399
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Berlin Heidelberg
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Yu Guo
               affiliation:
                     name:Waisman Center, University of Wisconsin-Madison
                     address:
                        name:Waisman Center, University of Wisconsin-Madison, Madison, USA
                        type:PostalAddress
                     type:Organization
                     name:University of Wisconsin-Madison
                     address:
                        name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xinyu Zhao
               affiliation:
                     name:Waisman Center, University of Wisconsin-Madison
                     address:
                        name:Waisman Center, University of Wisconsin-Madison, Madison, USA
                        type:PostalAddress
                     type:Organization
                     name:University of Wisconsin-Madison
                     address:
                        name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:CRISPR-based genetic screens in human pluripotent stem cells derived neurons and brain organoids
      description:Recent large-scale genome-wide association and single-cell RNA sequencing (scRNA-seq) studies have uncovered disease-associated genetic risk factors and cell type-specific genetic alterations. However, our understanding of how these genetic variants cause diseases and the underlying mechanisms remains largely unknown. Functional genomics screens using CRISPR-based technologies offer an effective tool for studying genes relevant to disease phenotypes. Here, we summarize recent CRISPR-based functional genomics screen approaches applied to human pluripotent stem cell (hPSC)-derived neurons and brain organoids. These screens have identified genes crucial for neurogenesis, neuronal survival, morphological development, and migration. Combining CRISPR-based genetic screens with scRNA-seq, researchers have revealed downstream genes and cellular pathways impacted by these genetic variants in human neural cells, providing new insights into the pathogenesis of neurodevelopmental disorders, such as microcephaly and autism spectrum disorders. Finally, we discuss current challenges and future directions for using CRISPR-based screens in furthering our understanding of neurological diseases and developing potential therapeutic strategies. Despite challenges, CRISPR-based screens have enormous potential for advancing the therapeutic development of many diseases.
      datePublished:2024-11-25T00:00:00Z
      dateModified:2024-11-25T00:00:00Z
      pageStart:1
      pageEnd:8
      sameAs:https://doi.org/10.1007/s00441-024-03934-2
      keywords:
         Genetic screens
         CRISPR
         Neurological diseases
         Neurons
         Organoids
         Human Genetics
         Proteomics
         Molecular Medicine
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-024-03934-2/MediaObjects/441_2024_3934_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00441-024-03934-2/MediaObjects/441_2024_3934_Fig2_HTML.jpg
      isPartOf:
         name:Cell and Tissue Research
         issn:
            1432-0878
            0302-766X
         volumeNumber:399
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Berlin Heidelberg
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Yu Guo
            affiliation:
                  name:Waisman Center, University of Wisconsin-Madison
                  address:
                     name:Waisman Center, University of Wisconsin-Madison, Madison, USA
                     type:PostalAddress
                  type:Organization
                  name:University of Wisconsin-Madison
                  address:
                     name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xinyu Zhao
            affiliation:
                  name:Waisman Center, University of Wisconsin-Madison
                  address:
                     name:Waisman Center, University of Wisconsin-Madison, Madison, USA
                     type:PostalAddress
                  type:Organization
                  name:University of Wisconsin-Madison
                  address:
                     name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Cell and Tissue Research
      issn:
         1432-0878
         0302-766X
      volumeNumber:399
Organization:
      name:Springer Berlin Heidelberg
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Waisman Center, University of Wisconsin-Madison
      address:
         name:Waisman Center, University of Wisconsin-Madison, Madison, USA
         type:PostalAddress
      name:University of Wisconsin-Madison
      address:
         name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
         type:PostalAddress
      name:Waisman Center, University of Wisconsin-Madison
      address:
         name:Waisman Center, University of Wisconsin-Madison, Madison, USA
         type:PostalAddress
      name:University of Wisconsin-Madison
      address:
         name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Yu Guo
      affiliation:
            name:Waisman Center, University of Wisconsin-Madison
            address:
               name:Waisman Center, University of Wisconsin-Madison, Madison, USA
               type:PostalAddress
            type:Organization
            name:University of Wisconsin-Madison
            address:
               name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
               type:PostalAddress
            type:Organization
      name:Xinyu Zhao
      affiliation:
            name:Waisman Center, University of Wisconsin-Madison
            address:
               name:Waisman Center, University of Wisconsin-Madison, Madison, USA
               type:PostalAddress
            type:Organization
            name:University of Wisconsin-Madison
            address:
               name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Waisman Center, University of Wisconsin-Madison, Madison, USA
      name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
      name:Waisman Center, University of Wisconsin-Madison, Madison, USA
      name:Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(210)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.35s.

LINK . SPRINGER . COM {}