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Title:
A carotenogenic gene cluster from Brevibacterium linens with novel lycopene cyclase genes involved in the synthesis of aromatic carotenoids | Molecular Genetics and Genomics
Description:
The carotenogenic (crt) gene cluster from Brevibacterium linens, a member of the commercially important group of coryneform bacteria, was cloned and identified. An expression library of B. linens genes was constructed and a fragment of the crt cluster was obtained by functional complementation of a colourless B. flavum mutant, screening transformed cells for production of a yellow pigment. Subsequent screening of a cosmid library resulted in the cloning of the wholecrt cluster from B. linens. All genes necessary for the synthesis of the aromatic carotenoid isorenieratene were identified on the basis of sequence homologies. In addition a novel type of lycopene cyclase was identified by complementation of a lycopene-accumulating B. flavum mutant. Two genes, named crtYc and crtYd, which code for polypeptides of 125 and 107 amino acids, respectively, are necessary to convert lycopene to β-carotene. The amino acid sequences of these polypeptides show no similarity to any of the known lycopene cyclases. This is the first example of a carotenoid biosynthetic conversion in which two different gene products are involved, probably forming a heterodimer.
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Keywords {🔍}
article, genes, lycopene, access, content, linens, privacy, cookies, cluster, information, publish, search, carotenogenic, gene, cyclase, biosynthesis, open, data, log, journal, research, genetics, brevibacterium, involved, synthesis, aromatic, krubasik, sandmann, identified, complementation, carotenoid, products, discover, springer, optional, analysis, personal, parties, policy, find, track, molecular, general, mgg, carotenoids, april, cite, explore, crt, group,
Topics {✒️}
month download article/chapter related subjects chlamydomonas reinhardtii carotenoid biosynthesis genes aromatic carotenoid isorenieratene lycopene cyclase functional complementation β-carotene content privacy choices/manage cookies full article pdf carotenogenic gene cluster check access instant access linens genes european economic area scope submit manuscript commercially important group carotenoid biosynthetic conversion deinococcus wulumuqiensis r12 β-carotene conditions privacy policy screening transformed cells cosmid library resulted amino acid sequences d-60054 frankfurt/main accepting optional cookies article molecular main content log journal finder publish lycopene-accumulating convert lycopene lycopene cyclases zeaxanthin biosynthesis aromatic carotenoids gene products article log usage analysis april 2000 volume 263 article cite article krubasik genes privacy policy personal data brevibacterium linens books a optional cookies manage preferences complementation gene cluster information
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headline:A carotenogenic gene cluster from Brevibacterium linens with novel lycopene cyclase genes involved in the synthesis of aromatic carotenoids
description: The carotenogenic (crt) gene cluster from Brevibacterium linens, a member of the commercially important group of coryneform bacteria, was cloned and identified. An expression library of B. linens genes was constructed and a fragment of the crt cluster was obtained by functional complementation of a colourless B. flavum mutant, screening transformed cells for production of a yellow pigment. Subsequent screening of a cosmid library resulted in the cloning of the wholecrt cluster from B. linens. All genes necessary for the synthesis of the aromatic carotenoid isorenieratene were identified on the basis of sequence homologies. In addition a novel type of lycopene cyclase was identified by complementation of a lycopene-accumulating B. flavum mutant. Two genes, named crtYc and crtYd, which code for polypeptides of 125 and 107 amino acids, respectively, are necessary to convert lycopene to β-carotene. The amino acid sequences of these polypeptides show no similarity to any of the known lycopene cyclases. This is the first example of a carotenoid biosynthetic conversion in which two different gene products are involved, probably forming a heterodimer.
datePublished:
dateModified:
pageStart:423
pageEnd:432
sameAs:https://doi.org/10.1007/s004380051186
keywords:
Key wordsBrevibacterium linens
Lycopene cyclase
Functional complementation
Isorenieratene
Carotenogenic genes
Plant Genetics and Genomics
Human Genetics
Microbial Genetics and Genomics
Animal Genetics and Genomics
Biochemistry
general
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headline:A carotenogenic gene cluster from Brevibacterium linens with novel lycopene cyclase genes involved in the synthesis of aromatic carotenoids
description: The carotenogenic (crt) gene cluster from Brevibacterium linens, a member of the commercially important group of coryneform bacteria, was cloned and identified. An expression library of B. linens genes was constructed and a fragment of the crt cluster was obtained by functional complementation of a colourless B. flavum mutant, screening transformed cells for production of a yellow pigment. Subsequent screening of a cosmid library resulted in the cloning of the wholecrt cluster from B. linens. All genes necessary for the synthesis of the aromatic carotenoid isorenieratene were identified on the basis of sequence homologies. In addition a novel type of lycopene cyclase was identified by complementation of a lycopene-accumulating B. flavum mutant. Two genes, named crtYc and crtYd, which code for polypeptides of 125 and 107 amino acids, respectively, are necessary to convert lycopene to β-carotene. The amino acid sequences of these polypeptides show no similarity to any of the known lycopene cyclases. This is the first example of a carotenoid biosynthetic conversion in which two different gene products are involved, probably forming a heterodimer.
datePublished:
dateModified:
pageStart:423
pageEnd:432
sameAs:https://doi.org/10.1007/s004380051186
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Key wordsBrevibacterium linens
Lycopene cyclase
Functional complementation
Isorenieratene
Carotenogenic genes
Plant Genetics and Genomics
Human Genetics
Microbial Genetics and Genomics
Animal Genetics and Genomics
Biochemistry
general
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