We are analyzing https://link.springer.com/article/10.1007/s004380050323.

Title:
A meiosis-specific protein kinase, Ime2, is required for the correct timing of DNA replication and for spore formation in yeast meiosis | Molecular Genetics and Genomics
Description:
In this report we study the regulation of premeiotic DNA synthesis in Saccharomyces cerevisiae. DNA replication was monitored by fluorescence-activated cell sorting analysis and by analyzing the pattern of expression of the DNA polymerase α-primase complex. Wild-type cells and cells lacking one of the two principal regulators of meiosis, Ime1 and Ime2, were compared. We show that premeiotic DNA synthesis does not occur in ime1Δ diploids, but does occur in ime2Δ diploids with an 8–9 h delay. At late meiotic times, ime2Δ diploids exhibit an additional round of DNA synthesis. Furthermore, we show that in wild-type cells the B-subunit of DNA polymerase α is phosphorylated during premeiotic DNA synthesis, a phenomenon that has previously been reported for the mitotic cell cycle. Moreover, the catalytic subunit and the B-subunit of DNA polymerase α are specifically degraded during spore formation. Phosphorylation of the B-subunit does not occur in ime1Δ diploids, but does occur in ime2Δ diploids with an 8–9 h delay. In addition, we show that Ime2 is not absolutely required for commitment to meiotic recombination, spindle formation and nuclear division, although it is required for spore formation.
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Keywords {🔍}

dna, article, meiosis, synthesis, privacy, cookies, content, ime, replication, formation, imeδ, diploids, access, information, publish, search, required, spore, polymerase, occur, data, log, journal, research, foiani, nadjarboger, capone, premeiotic, saccharomyces, cerevisiae, cells, show, meiotic, bsubunit, discover, springer, optional, analysis, personal, parties, policy, find, track, molecular, general, genetics, mgg, meiosisspecific, protein, kinase,

Topics {✒️}

mitotic cell cycle saccharomyces cerevisiae meiosis-specific protein kinase month download article/chapter dna polymerase α premeiotic dna synthesis late meiotic times dna replication related subjects privacy choices/manage cookies dna synthesis full article pdf european economic area scope submit manuscript yeast meiosis conditions privacy policy wild-type cells accepting optional cookies meiotic recombination main content log ime2δ diploids exhibit journal finder publish check access instant access article molecular privacy policy personal data usage analysis information books a article log meiosis optional cookies manage preferences additional round article cite article foiani data protection cells lacking essential cookies cookies skip subscription content similar content ime2δ diploids institution subscribe journal publish ime1δ diploids social media varying standards correct timing

Schema {🗺️}

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