We are analyzing https://link.springer.com/article/10.1007/s00432-012-1315-y.

Title:
Circulating microRNA-92a and microRNA-21 as novel minimally invasive biomarkers for primary breast cancer | Journal of Cancer Research and Clinical Oncology
Description:
MicroRNAs (miRNAs) play an essential role in breast malignant tumor development and progression. The development of clinically validated biomarkers for primary breast cancer (BC) has remained an insurmountable task despite other advances in the field of cancer molecular biology. The objective of this study is to investigate the differential expression of miRNAs and the potential of circulating microRNAs as novel primary breast cancer biomarkers. Our analyses were performed on 48 tissue and 100 serum samples of patients with primary BC and a set of 20 control samples of healthy women, respectively. The relative expression of ten candidate miRNAs (miR-106b, miR-125b, miR-17, miR-185, miR-21, miR-558, miR-625, miR-665, miR-92a, and miR-93) from the results of four bioinformatics approaches and literature curation was measured by real-time quantitative reverse transcription PCR (qRT-PCR). The level of miR-92a was significantly lower, while miR-21 was higher, as previous reports, in tissue and serum samples of BC than that of healthy controls (p < 0.001). Logistic regression and receiver operating characteristic curve analyses revealed the significant and independent value (p < 0.001) of the miR-92a and miR-21 expression quantification in serums. Moreover, the comparison with the clinicopathologic data of the BC patients showed that decreased levels of miR-92a and increased levels of miR-21 were associated with tumor size and a positive lymph node status (p < 0.001). These findings suggest that many miRNAs expressions are altered in BC, whose expression profiling may provide a useful clue for the pathophysiological research. Circulating miR-92a has potential use as novel breast cancer biomarker, which is comparable to miR-21.
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Keywords {🔍}

cancer, expression, mira, breast, article, mirnas, pubmed, google, scholar, cas, serum, patients, samples, circulating, biomarkers, levels, mirb, tissue, microrna, mirna, human, micrornas, results, tumor, potential, significant, rna, work, nats, cell, analysis, research, clinical, relative, qrtpcr, gene, klk, tissues, primary, chen, wang, serums, size, fig, resistance, chem, biol, res, data, access,

Topics {✒️}

mirna-specific forward primers huanchun wang & chengjin hu quantitative reverse transcription-pcr article download pdf real-time quantitative pcr tumor-lymph node-metastasis modulate platinum-based chemotherapy uni-mir qpcr primer diffuse b-cell lymphoma freeze–thaw cycle analyses small-cell lung cancer total rna derived mir-92a expression profiles cell-free nucleic acids yuan cao receiver operating characteristic lymph node metastasis stable blood-based biomarkers poor disease-free survival mir-92a expression pattern cancer-specific decrease mir-92a directly targeted lymph node metastases small expressed rnas privacy choices/manage cookies mir-21 expression profiles universal adaptor primer clinical oncology aims clinical histopathological features mirnas including mir-106b total rna isolation primary breast cancer mir-92a expression levels coding rna molecules full access human breast cancer large rna molecules direct serum assay breast malignant tumor regulate multiple genes anti-her2 therapy open access regulate klk5 expression advanced breast cancer breast cancer management metastatic breast cancer spearman-rho test circulating mir-125b expression characteristic expression patterns screening breast tumors

Schema {🗺️}

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         headline:Circulating microRNA-92a and microRNA-21 as novel minimally invasive biomarkers for primary breast cancer
         description:MicroRNAs (miRNAs) play an essential role in breast malignant tumor development and progression. The development of clinically validated biomarkers for primary breast cancer (BC) has remained an insurmountable task despite other advances in the field of cancer molecular biology. The objective of this study is to investigate the differential expression of miRNAs and the potential of circulating microRNAs as novel primary breast cancer biomarkers. Our analyses were performed on 48 tissue and 100 serum samples of patients with primary BC and a set of 20 control samples of healthy women, respectively. The relative expression of ten candidate miRNAs (miR-106b, miR-125b, miR-17, miR-185, miR-21, miR-558, miR-625, miR-665, miR-92a, and miR-93) from the results of four bioinformatics approaches and literature curation was measured by real-time quantitative reverse transcription PCR (qRT-PCR). The level of miR-92a was significantly lower, while miR-21 was higher, as previous reports, in tissue and serum samples of BC than that of healthy controls (p < 0.001). Logistic regression and receiver operating characteristic curve analyses revealed the significant and independent value (p < 0.001) of the miR-92a and miR-21 expression quantification in serums. Moreover, the comparison with the clinicopathologic data of the BC patients showed that decreased levels of miR-92a and increased levels of miR-21 were associated with tumor size and a positive lymph node status (p < 0.001). These findings suggest that many miRNAs expressions are altered in BC, whose expression profiling may provide a useful clue for the pathophysiological research. Circulating miR-92a has potential use as novel breast cancer biomarker, which is comparable to miR-21.
         datePublished:2012-09-30T00:00:00Z
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      headline:Circulating microRNA-92a and microRNA-21 as novel minimally invasive biomarkers for primary breast cancer
      description:MicroRNAs (miRNAs) play an essential role in breast malignant tumor development and progression. The development of clinically validated biomarkers for primary breast cancer (BC) has remained an insurmountable task despite other advances in the field of cancer molecular biology. The objective of this study is to investigate the differential expression of miRNAs and the potential of circulating microRNAs as novel primary breast cancer biomarkers. Our analyses were performed on 48 tissue and 100 serum samples of patients with primary BC and a set of 20 control samples of healthy women, respectively. The relative expression of ten candidate miRNAs (miR-106b, miR-125b, miR-17, miR-185, miR-21, miR-558, miR-625, miR-665, miR-92a, and miR-93) from the results of four bioinformatics approaches and literature curation was measured by real-time quantitative reverse transcription PCR (qRT-PCR). The level of miR-92a was significantly lower, while miR-21 was higher, as previous reports, in tissue and serum samples of BC than that of healthy controls (p < 0.001). Logistic regression and receiver operating characteristic curve analyses revealed the significant and independent value (p < 0.001) of the miR-92a and miR-21 expression quantification in serums. Moreover, the comparison with the clinicopathologic data of the BC patients showed that decreased levels of miR-92a and increased levels of miR-21 were associated with tumor size and a positive lymph node status (p < 0.001). These findings suggest that many miRNAs expressions are altered in BC, whose expression profiling may provide a useful clue for the pathophysiological research. Circulating miR-92a has potential use as novel breast cancer biomarker, which is comparable to miR-21.
      datePublished:2012-09-30T00:00:00Z
      dateModified:2012-09-30T00:00:00Z
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      pageEnd:229
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         microRNA
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         Internal Medicine
         Hematology
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