We are analyzing https://link.springer.com/article/10.1007/s00428-009-0728-8.

Title:
Comprehensive immunohistochemical analysis of Her-2/neu oncoprotein overexpression in breast cancer: HercepTest™ (Dako) for manual testing and Her-2/neuTest 4B5 (Ventana) for Ventana BenchMark automatic staining system with correlation to results of fluorescence in situ hybridization (FISH) | Virchows Archiv
Description:
Overexpression of Her-2/neu-oncoprotein is used as marker for Herceptin® therapy. To investigate the sensitivity and specificity of automatic immunohistochemistry (Benchmark, Ventana), we compared the results to the manual testing (Dako) in 130 breast carcinomas and validated the results by fluorescence in situ hybridization (FISH). Manual and automatic immunohistochemistry of Her-2/neu-oncoprotein using two different antibodies (HercepTest™, Her-2/neuTest 4B5) was analyzed. FISH was performed in all cases with uncertain or strong overexpression in either immunohistochemical stainings or with different immunohistochemical results. Same immunohistochemical results were seen in 73.8%. Two cases with overexpression, detected with Her-2/neuTest 4B5 and confirmed by FISH, showed no overexpression using HercepTest™. From 21 cases with 2+ by Her-2/neuTest 4B5, 15 cases had no gene amplification (two of them with 3+ HercepTest™); three cases showed a gene amplification (one of them with failing overexpression by HercepTest™); two other cases were polysomic; one could not be analyzed. Ventana immunohistochemistry seems to be of same reliability like Dako with a little better concordance to FISH in our study.
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Keywords {🔍}

article, breast, google, scholar, cancer, pubmed, cas, herneu, overexpression, immunohistochemical, clin, ventana, fish, herceptest, testing, results, fluorescence, situ, hybridization, gene, amplification, oncol, dako, therapy, cases, access, privacy, cookies, content, analysis, herneutest, mayr, immunohistochemistry, study, pathol, publish, search, manual, benchmark, automatic, correlation, carcinomas, molecular, american, data, information, log, journal, research, virchows,

Topics {✒️}

month download article/chapter paraffin-processed cell lines asco/cap guideline recommendations tissue microarray-based assessment comprehensive immunohistochemical analysis bowel project experience related subjects full article pdf privacy choices/manage cookies original article published molecular targeting agents comparative methodological analysis rabbit monoclonal antibody situ hybridization testing press mf clinical laboratory testing american pathologists guidelines article mayr european economic area real-world performance clinical oncology/college chromosomal copy number molecularly characterized cohort outstanding technical contribution her2 status conditions privacy policy breast cancer specimens ventana pathway anti hoffmann-la roche breast carcinoma tissues invasive ductal carcinoma detect her2 overexpression accepting optional cookies article log check access instant access invasive mammary carcinomas breast cancer therapy 2/neu assessment automatic immunohistochemistry journal finder publish virchows arch 454 herceptest™ mab pharmdx 2/neu gene amplification molecular study molecular pathway article cite doris mayr situ hybridization ventana immunohistochemistry

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         headline:Comprehensive immunohistochemical analysis of Her-2/neu oncoprotein overexpression in breast cancer: HercepTest™ (Dako) for manual testing and Her-2/neuTest 4B5 (Ventana) for Ventana BenchMark automatic staining system with correlation to results of fluorescence in situ hybridization (FISH)
         description:Overexpression of Her-2/neu-oncoprotein is used as marker for Herceptin® therapy. To investigate the sensitivity and specificity of automatic immunohistochemistry (Benchmark, Ventana), we compared the results to the manual testing (Dako) in 130 breast carcinomas and validated the results by fluorescence in situ hybridization (FISH). Manual and automatic immunohistochemistry of Her-2/neu-oncoprotein using two different antibodies (HercepTest™, Her-2/neuTest 4B5) was analyzed. FISH was performed in all cases with uncertain or strong overexpression in either immunohistochemical stainings or with different immunohistochemical results. Same immunohistochemical results were seen in 73.8%. Two cases with overexpression, detected with Her-2/neuTest 4B5 and confirmed by FISH, showed no overexpression using HercepTest™. From 21 cases with 2+ by Her-2/neuTest 4B5, 15 cases had no gene amplification (two of them with 3+ HercepTest™); three cases showed a gene amplification (one of them with failing overexpression by HercepTest™); two other cases were polysomic; one could not be analyzed. Ventana immunohistochemistry seems to be of same reliability like Dako with a little better concordance to FISH in our study.
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         Breast cancer
         Her-2/neu-immunohistochemistry
         BenchMark
         FISH
         Pathology
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