We are analyzing https://link.springer.com/article/10.1007/s004250050069.

Title:
Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance | Planta
Description:
The yeast trehalose-6-phosphate synthase gene (TPS1) was engineered under the control of the cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants. Using Agrobacterium-mediated transfer, the gene was incorporated into the genomic DNA and constitutively expressed in Nicotiana tabacum L. plants. Trehalose was determined in the transformants, by anion-exchange chromatography coupled to pulsed amperometric detection. The non-reducing disaccharide accumulated up to 0.17 mg per g fresh weight in leaf extracts of transgenic plants. Trehaloseaccumulating plants exhibited multiple phenotypic alterations, including stunted growth, lancet-shaped leaves, reduced sucrose content and improved drought tolerance. These pleiotropic effects, and the fact that water loss from detached leaves was not significantly affected by trehalose accumulation, suggest that synthesis of this sugar, rather than leading to an osmoprotectant effect, had altered sugar metabolism and regulatory pathways affecting plant development and stress tolerance.
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Keywords {🔍}

article, google, scholar, cas, plant, plants, trehalose, pubmed, yeast, gene, tolerance, transgenic, content, tobacco, expression, synthase, drought, stress, physiol, privacy, cookies, trehalosephosphate, access, role, cell, publish, search, josé, leaves, sucrose, molecular, boller, wiemken, nature, data, information, log, journal, research, planta, pleiotropic, romero, bellés, vayá, serrano, francisco, culiáñezmacià, cauliflower, mosaic, virus,

Topics {✒️}

month download article/chapter trehalose stabilizes membrane-structure yeast trehalose-6-phosphate synthase anion-exchange chromatography coupled transgenic tobacco plants drought stress tolerance transgenic tobacco leads sucrose phosphate synthase improved drought tolerance article planta aims cold spring harbor full article pdf desiccation tolerance correlated abiotic stress tolerance privacy choices/manage cookies plant storing starch consensus sequences preceding anion exchange chromatography celular de plantas camino de vera transgenic plants binary agrobacterium vectors tobacco tissue cultures agrobacterium-mediated transfer gene family reveals bacterial mtld gene drought tolerance european economic area nicotiana tabacum pulsed amperometric detection reducing disaccharide accumulated carbon catabolite inactivation translation initiator codon dehydrating pollen species hu c-aa high voltage electroporation ramón serrano & francisco article romero check access instant access conditions privacy policy reduced sucrose content including stunted growth correlative evidence suggesting plant sci 112 stress tolerance van der zee accepting optional cookies related subjects lancet-shaped leaves

Questions {❓}

Hoekstra FA, Crowe LM, Van Rockel T, Vermeer E (1992) Do phospholipids and sucrose determine membrane phase transitions in dehydrating pollen species?

Schema {🗺️}

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            Plant Sciences
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      headline:Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance
      description:The yeast trehalose-6-phosphate synthase gene (TPS1) was engineered under the control of the cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants. Using Agrobacterium-mediated transfer, the gene was incorporated into the genomic DNA and constitutively expressed in Nicotiana tabacum L. plants. Trehalose was determined in the transformants, by anion-exchange chromatography coupled to pulsed amperometric detection. The non-reducing disaccharide accumulated up to 0.17 mg per g fresh weight in leaf extracts of transgenic plants. Trehaloseaccumulating plants exhibited multiple phenotypic alterations, including stunted growth, lancet-shaped leaves, reduced sucrose content and improved drought tolerance. These pleiotropic effects, and the fact that water loss from detached leaves was not significantly affected by trehalose accumulation, suggest that synthesis of this sugar, rather than leading to an osmoprotectant effect, had altered sugar metabolism and regulatory pathways affecting plant development and stress tolerance.
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         Nicotiana
         Osmotic stress
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