We are analyzing https://link.springer.com/article/10.1007/s00345-007-0166-7.

Title:
A new and reliable culture system for superficial low-grade urothelial carcinoma of the bladder | World Journal of Urology
Description:
Several bladder cancer culture systems have been developed in recent years. However, reports about successful primary cultures of superficial urothelial carcinomas (UC) are sparse. Based on the specific growth requirements of UC described previously, we developed a new and reliable culture system for superficial low-grade UC. Between November 2002 and April 2006, 64 primary cultures of bladder cancer specimens were performed. After incubating the specimens overnight in 0.1% ethylenediaminetetraacetic acid solution, tumour cells could easily be separated from the submucosal tissue. Subsequently, cells were seeded in a low-calcium culture medium supplemented with 1% serum, growth factors, non-essential amino acids and glycine. The malignant origin of the cultured cells was demonstrated by spectral karyotyping. Overall culture success rate leading to a homogenous tumour cell population without fibroblast contamination was 63%. Culture success could be remarkably enhanced by the addition of glycine to the culture medium. Interestingly, 86.4% of pTa tumours were cultured successfully compared to only 50% of the pT1 and 38% of advanced stage tumours, respectively. G1 and G2 tumours grew significantly better than G3 tumours (86, 73 and 41%, respectively). Up to three passages of low-grade UC primary cultures were possible. We describe a new and reliable culture system, which is highly successful for primary culture and passage of low-grade UC of the bladder. Therefore, this culture system can widely be used for functional experiments on early stage bladder cancer.
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article, cancer, google, scholar, pubmed, cas, bladder, culture, human, urothelial, cells, cell, primary, carcinoma, growth, lowgrade, urol, superficial, seifert, tumours, res, vitro, privacy, cookies, content, journal, system, cronauer, cultures, access, publish, search, world, reliable, hanshelge, schulz, tissue, genes, chromosomes, university, düsseldorf, data, information, log, research, urology, april, meyer, marcus, rieder,

Topics {✒️}

month download article/chapter superficial low-grade uc heinrich-heine university düsseldorf transitional cell carcinoma beta1-integrin-mediated attachment superficial urothelial carcinomas low-grade uc human urological tumors urothelial cell cultures human bladder cancer hans-helge seifert tissue culture model bladder cancer specimens full article pdf privacy choices/manage cookies article world journal human cancer cells bladder wound healing human bladder carcinogenesis human cancer pathogenesis reliable culture system normal urothelial cells selective culture conditions human tumor cells successful primary cultures bladder cancer short-term cultures urothelial cancer european economic area cultured successfully compared advanced stage tumours holistic integrative management related subjects accumulated allelic losses jimenez-cruz jf short-term culturing intraluminal recurrence rates map-kinase pathways lower urinary tract overcoming cellular senescence hepatocyte survival depends rolf ackermann & wolfgang normal uroepithelial cells article seifert primary culture essential amino acids molecular genetic analysis specific growth requirements altered growth pattern conditions privacy policy

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         headline:A new and reliable culture system for superficial low-grade urothelial carcinoma of the bladder
         description:Several bladder cancer culture systems have been developed in recent years. However, reports about successful primary cultures of superficial urothelial carcinomas (UC) are sparse. Based on the specific growth requirements of UC described previously, we developed a new and reliable culture system for superficial low-grade UC. Between November 2002 and April 2006, 64 primary cultures of bladder cancer specimens were performed. After incubating the specimens overnight in 0.1% ethylenediaminetetraacetic acid solution, tumour cells could easily be separated from the submucosal tissue. Subsequently, cells were seeded in a low-calcium culture medium supplemented with 1% serum, growth factors, non-essential amino acids and glycine. The malignant origin of the cultured cells was demonstrated by spectral karyotyping. Overall culture success rate leading to a homogenous tumour cell population without fibroblast contamination was 63%. Culture success could be remarkably enhanced by the addition of glycine to the culture medium. Interestingly, 86.4% of pTa tumours were cultured successfully compared to only 50% of the pT1 and 38% of advanced stage tumours, respectively. G1 and G2 tumours grew significantly better than G3 tumours (86, 73 and 41%, respectively). Up to three passages of low-grade UC primary cultures were possible. We describe a new and reliable culture system, which is highly successful for primary culture and passage of low-grade UC of the bladder. Therefore, this culture system can widely be used for functional experiments on early stage bladder cancer.
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            Low-grade urothelial cancer
            Primary culture
            Urology
            Nephrology
            Oncology
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      headline:A new and reliable culture system for superficial low-grade urothelial carcinoma of the bladder
      description:Several bladder cancer culture systems have been developed in recent years. However, reports about successful primary cultures of superficial urothelial carcinomas (UC) are sparse. Based on the specific growth requirements of UC described previously, we developed a new and reliable culture system for superficial low-grade UC. Between November 2002 and April 2006, 64 primary cultures of bladder cancer specimens were performed. After incubating the specimens overnight in 0.1% ethylenediaminetetraacetic acid solution, tumour cells could easily be separated from the submucosal tissue. Subsequently, cells were seeded in a low-calcium culture medium supplemented with 1% serum, growth factors, non-essential amino acids and glycine. The malignant origin of the cultured cells was demonstrated by spectral karyotyping. Overall culture success rate leading to a homogenous tumour cell population without fibroblast contamination was 63%. Culture success could be remarkably enhanced by the addition of glycine to the culture medium. Interestingly, 86.4% of pTa tumours were cultured successfully compared to only 50% of the pT1 and 38% of advanced stage tumours, respectively. G1 and G2 tumours grew significantly better than G3 tumours (86, 73 and 41%, respectively). Up to three passages of low-grade UC primary cultures were possible. We describe a new and reliable culture system, which is highly successful for primary culture and passage of low-grade UC of the bladder. Therefore, this culture system can widely be used for functional experiments on early stage bladder cancer.
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      pageStart:297
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         Low-grade urothelial cancer
         Primary culture
         Urology
         Nephrology
         Oncology
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