We are analyzing https://link.springer.com/article/10.1007/s00335-009-9240-3.

Title:
Conservation of miR-15a/16-1 and miR-15b/16-2 clusters | Mammalian Genome
Description:
MiR-15a/16-1 and miR-15b/16-2 clusters have been shown to play very important roles in regulating cell proliferation and apoptosis by targeting cell cycle proteins and the antiapoptotic Bcl-2 gene. However, the physiological implications of those two clusters are largely elusive. By aligning the primary miR-15a/16-1 sequence among 44 vertebrates, we found that there was a gap in the homologous region of the rat genome. To verify that there was a similar miR-15a/16-1 cluster in rats, we amplified this region from rat genomic DNA using PCR and found that a 697-bp sequence was missing in the current rat genome database, which covers the miR-15a/16-1 cluster. Subsequently, we also investigated the expression pattern of individual miRNAs spliced from miR-15a/16-1 and miR-15b/16-2 clusters, including miR-15a, miR-15a*, miR-15b, miR-15b*, miR-16-1/2, and miR-16-1/2* from various rat tissues, and found that all of those miRNAs were expressed in the investigated tissues. MiR-16 was most expressed in the heart, followed by the brain, lung, kidney, and small intestine, which indicates tissue specificity for individual miRNA expression from both clusters. Our results demonstrated that both miR-15a/16-1 and miR-15b/16-2 clusters are highly conserved among mammalian species. The investigation of the biological functions of those two clusters using transgenic or knockout/knockdown models will provide new clues to understanding their implications in human diseases and finding a new approach for miRNA-based therapy.
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Keywords {🔍}

article, cas, pubmed, google, scholar, cell, mira, cancer, microrna, mirb, clusters, human, nature, content, genome, cycle, access, genes, privacy, cookies, yue, gene, rat, expression, cells, res, publish, search, targeting, cluster, dna, functions, smc, biol, hirano, health, author, essential, data, information, log, journal, research, mammalian, december, junming, tigyi, proliferation, apoptosis, proteins,

Topics {✒️}

chronic lymphocytic leukemia month download article/chapter critical host gene smc5-smc6 complex involved primary mir-15a/16-1 sequence high bic/mir-155 expression similar mir-15a/16-1 cluster related subjects full article pdf cell cycle regulation mir-15b/16-2 clusters published antiapoptotic bcl-2 gene leuk lymphoma 50 privacy choices/manage cookies regulating cell proliferation cell death differ human leukemia mir-15a/16-1 cluster article yue junming yue including mir-15a circulating tumor cells pleiotropically acting microrna mir-16–1 cluster functions european economic area 697-bp sequence knockout/knockdown models combining network based rb-dependent manner liu b hinge-mediated dimerization central nervous system conditions privacy policy dynamic molecular linkers individual mirnas spliced epigenetic memory mirnas proliferation centres characterized check access electronic supplementary material instant access article log rat genomic dna mitochondrial dna depletion mirna-based therapy microrna-15/107 family accepting optional cookies glioblastoma stem cells mir-15b/16-2 clusters individual mirna expression gene nomenclature

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         headline:Conservation of miR-15a/16-1 and miR-15b/16-2 clusters
         description:MiR-15a/16-1 and miR-15b/16-2 clusters have been shown to play very important roles in regulating cell proliferation and apoptosis by targeting cell cycle proteins and the antiapoptotic Bcl-2 gene. However, the physiological implications of those two clusters are largely elusive. By aligning the primary miR-15a/16-1 sequence among 44 vertebrates, we found that there was a gap in the homologous region of the rat genome. To verify that there was a similar miR-15a/16-1 cluster in rats, we amplified this region from rat genomic DNA using PCR and found that a 697-bp sequence was missing in the current rat genome database, which covers the miR-15a/16-1 cluster. Subsequently, we also investigated the expression pattern of individual miRNAs spliced from miR-15a/16-1 and miR-15b/16-2 clusters, including miR-15a, miR-15a*, miR-15b, miR-15b*, miR-16-1/2, and miR-16-1/2* from various rat tissues, and found that all of those miRNAs were expressed in the investigated tissues. MiR-16 was most expressed in the heart, followed by the brain, lung, kidney, and small intestine, which indicates tissue specificity for individual miRNA expression from both clusters. Our results demonstrated that both miR-15a/16-1 and miR-15b/16-2 clusters are highly conserved among mammalian species. The investigation of the biological functions of those two clusters using transgenic or knockout/knockdown models will provide new clues to understanding their implications in human diseases and finding a new approach for miRNA-based therapy.
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      description:MiR-15a/16-1 and miR-15b/16-2 clusters have been shown to play very important roles in regulating cell proliferation and apoptosis by targeting cell cycle proteins and the antiapoptotic Bcl-2 gene. However, the physiological implications of those two clusters are largely elusive. By aligning the primary miR-15a/16-1 sequence among 44 vertebrates, we found that there was a gap in the homologous region of the rat genome. To verify that there was a similar miR-15a/16-1 cluster in rats, we amplified this region from rat genomic DNA using PCR and found that a 697-bp sequence was missing in the current rat genome database, which covers the miR-15a/16-1 cluster. Subsequently, we also investigated the expression pattern of individual miRNAs spliced from miR-15a/16-1 and miR-15b/16-2 clusters, including miR-15a, miR-15a*, miR-15b, miR-15b*, miR-16-1/2, and miR-16-1/2* from various rat tissues, and found that all of those miRNAs were expressed in the investigated tissues. MiR-16 was most expressed in the heart, followed by the brain, lung, kidney, and small intestine, which indicates tissue specificity for individual miRNA expression from both clusters. Our results demonstrated that both miR-15a/16-1 and miR-15b/16-2 clusters are highly conserved among mammalian species. The investigation of the biological functions of those two clusters using transgenic or knockout/knockdown models will provide new clues to understanding their implications in human diseases and finding a new approach for miRNA-based therapy.
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         Host Gene
         Mature Sequence
         Cell Biology
         Animal Genetics and Genomics
         Human Genetics
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