We are analyzing https://link.springer.com/article/10.1007/s00294-018-0862-4.

Title:
Novel insights into global translational regulation through Pumilio family RNA-binding protein Puf3p revealed by ribosomal profiling | Current Genetics
Description:
RNA binding proteins (RBPs) can regulate the stability, localization, and translation of their target mRNAs. Among them, Puf3p is a well-known Pumilio family RBP whose biology has been intensively studied. Nevertheless, the impact of Puf3p on the translational regulation of its downstream genes still remains to be investigated at the genome-wide level. In this study, we combined ribosome profiling and RNA-Seq in budding yeast (Saccharomyces cerevisiae) to investigate Puf3p’s functions in translational regulation. Comparison of translational efficiency (TE) between wild-type and puf3Δ strains demonstrates extensive translational modulation in the absence of Puf3p (over 27% genes are affected at the genome level). Besides confirming its known role in regulating mitochondrial metabolism, our data demonstrate that Puf3p serves as a key post-transcriptional regulator of downstream RBPs by regulating their translational efficiencies, indicating a network of interactions among RBPs at the post-transcriptional level. Furthermore, Puf3p switches the balance of translational flux between mitochondrial and cytosolic ribosome biogenesis to adapt to changes in cellular metabolism. In summary, our results indicate that TE can be utilized as an informative index to interrogate the mechanism underlying RBP functions, and provide novel insights into Puf3p’s mode-of-action.
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Keywords {🔍}

pubmed, article, google, scholar, cas, central, protein, wang, rna, translational, yeast, regulation, pufp, biol, puf, proteins, supplementary, material, profiling, zhe, cerevisiae, mitochondrial, posttranscriptional, cell, res, translation, saccharomyces, mol, data, rnabinding, sun, ribosome, access, genet, httpsdoiorgs, nucleic, acids, mrna, xlsx, binding, mrnas, genome, nature, gene, expression, plos, curr, privacy, cookies, content,

Topics {✒️}

human pumilio-homology domain predicted protein-protein associations conserved rna-binding protein month download article/chapter protein-protein interaction networks key post-transcriptional regulator post-transcriptional rna regulons carbon source-dependent alteration pumilio family rbp selective rna-protein interactions rna-protein interaction network xiaoxian guo designed rna binding proteins rna-binding proteins conducted rna-seq studies protein–protein interaction puf-rna binding full article pdf metabolic cycle-regulated gene protein synthesis post-transcriptional regulation puf family portrait protein-protein interactions ingolia nt transcript-specific regulator post-transcriptional events post-transcriptional level high-resolution view privacy choices/manage cookies related subjects article wang zhe wang analyzed yeast saccharomyces cerevisiae saccharomyces cerevisiae transcriptome nucleotide resolution zhenglong gu wrote zhenglong gu initiated cytotopically related mrnas global translational regulation combined ribosome profiling translational elongation cycle glucose-regulated phosphorylation regulates sexual fates mitochondrial translational-initiation ribosome profiling data article log curr protoc bioinforma mitochondrial proteins check access instant access

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         description:RNA binding proteins (RBPs) can regulate the stability, localization, and translation of their target mRNAs. Among them, Puf3p is a well-known Pumilio family RBP whose biology has been intensively studied. Nevertheless, the impact of Puf3p on the translational regulation of its downstream genes still remains to be investigated at the genome-wide level. In this study, we combined ribosome profiling and RNA-Seq in budding yeast (Saccharomyces cerevisiae) to investigate Puf3p’s functions in translational regulation. Comparison of translational efficiency (TE) between wild-type and puf3Δ strains demonstrates extensive translational modulation in the absence of Puf3p (over 27% genes are affected at the genome level). Besides confirming its known role in regulating mitochondrial metabolism, our data demonstrate that Puf3p serves as a key post-transcriptional regulator of downstream RBPs by regulating their translational efficiencies, indicating a network of interactions among RBPs at the post-transcriptional level. Furthermore, Puf3p switches the balance of translational flux between mitochondrial and cytosolic ribosome biogenesis to adapt to changes in cellular metabolism. In summary, our results indicate that TE can be utilized as an informative index to interrogate the mechanism underlying RBP functions, and provide novel insights into Puf3p’s mode-of-action.
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         general
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         Plant Sciences
         Proteomics
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