We are analyzing https://link.springer.com/article/10.1007/s00277-025-06252-x.

Title:
Decoding HbF reactivation by hydroxyurea in hemoglobinopathy patients through microRNA signatures | Annals of Hematology
Description:
Hydroxyurea promotes HbF elevation in b-thalassemia and sickle cell anemia patients however, its mechanism is not completely elucidated. Previous studies have associated microRNAs with the regulation of HBG2, prompting this study to investigate specific microRNAs linked to HbF regulation in patients treated with hydroxyurea. 150 patients were recruited in the study. miRNA microarray panel of 754 miRNAs was used, and a further customised microarray card was designed. In silico tools predicted target genes of miRNAs and in vitro transfection functionally validated miRNAs in erythroblast cells from patients and K562 cells. Global microarray revealed 59 differentially expressed miRNAs, with 42 upregulated and 17 downregulated significantly following 3/6 months of hydroxyurea treatment (p < 0.001). Further, the customised miRNA panel of these 59 miRNAs confirmed the results. Among 59 miRNAs, 12 upregulated and 3 downregulated miRNAs correlated with HBG2 expression and HbF levels. In silico predictions and in vitro cells study identified miR-150, miR-155, miR-374, miR-486-3p (BCL11A, MYB); miR-190, miR-26a, miR-30b, miR-362, miR-411 (BCL11A); miR-192, miR-454 (MYB); miR-326 (KLF1); miR-150 (GATA1) and miR-15a, miR-484, miR-105 commonly share BCL11A, MYB, KLF1 and GATA1. Our study uncovers how microRNAs influence fetal hemoglobin induction in patients with hydroxyurea treatment, thus offering insights for developing treatment strategies and alleviating clinical severity.
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Keywords {🔍}

patients, expression, mirnas, hbf, mirna, hydroxyurea, cells, cell, article, study, bthalassemia, sickle, treatment, gene, pubmed, target, clinical, γglobin, google, scholar, genes, levels, bcla, myb, baseline, fig, months, hemoglobin, anemia, major, analysis, hbg, thalassemia, intermedia, table, mira, fetal, cas, severity, supplementary, klf, sca, transfection, gata, globin, human, score, control, pathways, journal,

Topics {✒️}

key=35a72c9e-8f64-4f06-8fc3-68158896943a] uk/biostudies/arrayexpress/studies/e-mtab-14361 β0-thalassemia/hbe disease linear mixed-effects model identify disease-mediated dysregulation article download pdf hbe/β-thalassemia patients sickle cell disease sickle cell disease wilcoxon signed-rank test customised microarray card sickle cell anemia sickle cell homozygous magnetically labelled cd71 + microbeads empty vector/mock transfection full access b-thalassemia major group induce γ-globin expression small rna molecules b-thalassemia intermedia patients global mirna microarray responders b-thalassemia patients protein-protein interaction network hydroxyurea-induced mirna expression γ-globin gene expression mir-486-3p commonly share regulation induces γ-globin mirna microarray panel increased γ-globin compared phenotypic scoring system article kargutkar mirna microarray analysis icmr-national institute γ-globin gene modulation privacy choices/manage cookies hbs homozygous patient mirna-mediated pathways involved global population carries 20 b-thalassemia major b-thalassemia major b- thalassemia major b-thalassemia patients b-thalassemia patients [23] microrna expression patterns mixed model analysis customised mirna panel hbf levels/hbg2 expression 50 b-thalassemia intermedia 20 b-thalassemia intermedia b-thalassemia intermedia

Questions {❓}

Does HbF induction by hydroxycarbamide work through MIR210 in sickle cell anaemia patients?
Uk/biostudies/arrayexpress/studies/E-MTAB-14361?

Schema {🗺️}

WebPage:
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         headline:Decoding HbF reactivation by hydroxyurea in hemoglobinopathy patients through microRNA signatures
         description:Hydroxyurea promotes HbF elevation in b-thalassemia and sickle cell anemia patients however, its mechanism is not completely elucidated. Previous studies have associated microRNAs with the regulation of HBG2, prompting this study to investigate specific microRNAs linked to HbF regulation in patients treated with hydroxyurea. 150 patients were recruited in the study. miRNA microarray panel of 754 miRNAs was used, and a further customised microarray card was designed. In silico tools predicted target genes of miRNAs and in vitro transfection functionally validated miRNAs in erythroblast cells from patients and K562 cells. Global microarray revealed 59 differentially expressed miRNAs, with 42 upregulated and 17 downregulated significantly following 3/6 months of hydroxyurea treatment (p < 0.001). Further, the customised miRNA panel of these 59 miRNAs confirmed the results. Among 59 miRNAs, 12 upregulated and 3 downregulated miRNAs correlated with HBG2 expression and HbF levels. In silico predictions and in vitro cells study identified miR-150, miR-155, miR-374, miR-486-3p (BCL11A, MYB); miR-190, miR-26a, miR-30b, miR-362, miR-411 (BCL11A); miR-192, miR-454 (MYB); miR-326 (KLF1); miR-150 (GATA1) and miR-15a, miR-484, miR-105 commonly share BCL11A, MYB, KLF1 and GATA1. Our study uncovers how microRNAs influence fetal hemoglobin induction in patients with hydroxyurea treatment, thus offering insights for developing treatment strategies and alleviating clinical severity.
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      headline:Decoding HbF reactivation by hydroxyurea in hemoglobinopathy patients through microRNA signatures
      description:Hydroxyurea promotes HbF elevation in b-thalassemia and sickle cell anemia patients however, its mechanism is not completely elucidated. Previous studies have associated microRNAs with the regulation of HBG2, prompting this study to investigate specific microRNAs linked to HbF regulation in patients treated with hydroxyurea. 150 patients were recruited in the study. miRNA microarray panel of 754 miRNAs was used, and a further customised microarray card was designed. In silico tools predicted target genes of miRNAs and in vitro transfection functionally validated miRNAs in erythroblast cells from patients and K562 cells. Global microarray revealed 59 differentially expressed miRNAs, with 42 upregulated and 17 downregulated significantly following 3/6 months of hydroxyurea treatment (p < 0.001). Further, the customised miRNA panel of these 59 miRNAs confirmed the results. Among 59 miRNAs, 12 upregulated and 3 downregulated miRNAs correlated with HBG2 expression and HbF levels. In silico predictions and in vitro cells study identified miR-150, miR-155, miR-374, miR-486-3p (BCL11A, MYB); miR-190, miR-26a, miR-30b, miR-362, miR-411 (BCL11A); miR-192, miR-454 (MYB); miR-326 (KLF1); miR-150 (GATA1) and miR-15a, miR-484, miR-105 commonly share BCL11A, MYB, KLF1 and GATA1. Our study uncovers how microRNAs influence fetal hemoglobin induction in patients with hydroxyurea treatment, thus offering insights for developing treatment strategies and alleviating clinical severity.
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      dateModified:2025-04-25T00:00:00Z
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         miRNA
         HbF
         Hydroxyurea
         Thalassemia
         Sickle cell anemia
         Hematology
         Oncology
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      name:Anita Nadkarni
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               name:ICMR-National Institute of Immunohaematology, Parel, India
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