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Keywords {🔍}

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Topics {✒️}

tumour-specific mhc-class-ii-restricted responses effective mhc-class-ii-restricted recognition hla-dr-matched normal donors' synthetic peptide antigens potential fas/fas-ligand interactions targets survivin-2b80-88/hla month download article/chapter peptide-mhc hla-dr molecules mhc class tumour immunology group article cancer immunology coexpressing high levels annexin ii eluted full article pdf privacy choices/manage cookies hla-drb102x hubert kalbacher related subjects fas agonist m33 article li european economic area tcr-mimic antibody antigen-specific conditions privacy policy thomas halder medical research cho cells cotransfected tübingen medical school check access instant access cell response hla-drb10401 accepting optional cookies claudia müller isolated drb10401 molecules immunotherapy aims specific sensitization article log journal finder publish august 1998 volume 47 susanne heinzel fas ligand synthetic peptides article cite human molecules melanoma cells fm3 cells privacy policy personal data

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         headline:Tumour-specific MHC-class-II-restricted responses after in vitro sensitization to synthetic peptides corresponding to gp100 and Annexin II eluted from melanoma cells
         description: In a search for potentially tumour-specific MHC-class-II-restricted antigens, the immunogenicity of endogenous peptides that had been eluted from HLA-DR molecules of the human melanoma cell line FM3 (HLA-DRB1*02x, DRB1*0401) was tested in vitro. Two 16-mers representing gp100 positions 44–59, and annexin II positions 208–223 bound well to isolated DRB1*0401 molecules and are discussed here. HLA-DR-matched normal donors' T cells were cultured with peptide-pulsed artificial antigen-presenting cells (CHO cells cotransfected with genes for HLA-DRB1*0401 and CD80 and coexpressing high levels of both human molecules). Specific sensitization was achieved against both peptides, as measured in assays of autocrine proliferation and interleukin-2 secretion. Moreover, responses to native autologous melanoma cells but not to autologous B cells were also observed. In view of the expression of fas by the activated T cells and of fas ligand by the melanoma cells, blockade of potential fas/fas-ligand interactions was undertaken using monoclonal antibodies (mAb). The antagonistic fas-specific mAb M3, but not the fas agonist M33, caused a markedly enhanced T cell response to FM3 cells. These results demonstrate that synthetic peptide antigens are able to sensitize T cells in vitro for effective MHC-class-II-restricted recognition of melanoma cells.
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      headline:Tumour-specific MHC-class-II-restricted responses after in vitro sensitization to synthetic peptides corresponding to gp100 and Annexin II eluted from melanoma cells
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         Tumour escape from immune responses
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         Immunology
         Cancer Research
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Analytics and Tracking {📊}

• Google Tag Manager

Libraries {📚}

• Clipboard.js
• Prism.js

CDN Services {📦}

• Crossref