We are analyzing https://link.springer.com/article/10.1007/s00262-012-1250-4.

Title:
Annexin-V promotes anti-tumor immunity and inhibits neuroblastoma growth in vivo | Cancer Immunology, Immunotherapy
Description:
The goal of the current study is to determine the effects of blocking phosphatidylserine (PS) on the growth of neuroblastoma in mice. PS, an anionic phospholipid restricted to the cytoplasmic surface of plasma membranes in most cells, is externalized to the surface of apoptotic cells. PS has been shown to induce immune tolerance to self-antigens. PS can also be found on the surface of live cells and in particular tumor cells. Annexin-V (AnV) is a protein that specifically binds and blocks PS. To determine the effects of blocking PS with AnV on tumor growth and immunogenicity, mice were inoculated with AGN2a, a poorly immunogenic murine neuroblastoma that expresses high level of PS on the cell surface. Survival and anti-tumor T cell response were determined. AGN2a were engineered to secrete AnV. Secreted protein effectively blocked tumor PS. 40 % of mice inoculated with AnV-expressing AGN2a cells survived free of tumor, whereas none of the mice inoculated with control cells survived (p = 0.0062). The benefits of AnV were lost when mice were depleted of T cells. The findings suggest that AnV could protect mice from tumor challenge through an immune mediated mechanism. Mice were then immunized with irradiated AnV-secreting or control cells, and challenged with wild-type AGN2a cells. AnV-secreting cell vaccine protected 80 % of mice from AGN2a challenge, while control cell vaccine prevented tumor growth in only 30 % of animals (p = 0.012). ELISPOT analysis demonstrated that AnV-secreting cell vaccine induced a greater frequency of interferon-gamma producing splenic T cells. T cells isolated from mice immunized with AnV-secreting but not control vaccine lysed AGN2a. In summary, AnV blocked PS, enhanced T cell mediated tumor immunity, and inhibited tumor growth.
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article, pubmed, google, scholar, cas, cells, tumor, cell, cancer, mice, phosphatidylserine, immunol, immunity, neuroblastoma, vaccine, apoptotic, growth, inhibits, yan, johnson, shilyansky, surface, immune, anv, agna, access, human, privacy, cookies, content, antitumor, vivo, doffek, dendritic, res, usa, nat, herrmann, biol, function, publish, search, annexinv, promotes, yin, michael, sugg, joel, anionic, immunogenicity,

Topics {✒️}

t-cell-dependent immune defense tumor-induced t-cell tolerance murine cd8 + tumor-infiltrating lymphocytes apoptotic human t-cells month download article/chapter cd8 + ctl tumor immunosurveillance interferon-gamma producing splenic related subjects impaired ifn-gamma production de novo-induced regulatory promotes anti-tumor immunity radiation-enhanced vascular targeting wild-type agn2a cells mammary tumor-bearing mice freeman gj full article pdf anionic phospholipids serves article cancer immunology control cells survived mouse tumor vaccine monoclonal anti-phosphatidylserine antibody tumor blood vessels binds anionic phospholipids human dendritic cells privacy choices/manage cookies abc1 promotes engulfment neuroblastoma cells inhibit mammary tumor cells joel shilyansky inhibits neuroblastoma growth interferon alpha-induced tumor vaccine superior dying tumor cells phosphatidylserine-dependent ingestion helper-2 phenotype tumor-specific tolerance cognate tumor immunity induces tumor immunity induce immune tolerance gemcitabine strongly inhibits anti-tumor activity tumor necrosis factor human lung cancers irradiated lymphoma cells cells contribute independently johnson bd tumor regressions mediated inhibited tumor growth induces autoantibody production irradiated anv-secreting

Questions {❓}

Henson PM, Bratton DL, Fadok VA (2001) The phosphatidylserine receptor: a crucial molecular switch?

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         description:The goal of the current study is to determine the effects of blocking phosphatidylserine (PS) on the growth of neuroblastoma in mice. PS, an anionic phospholipid restricted to the cytoplasmic surface of plasma membranes in most cells, is externalized to the surface of apoptotic cells. PS has been shown to induce immune tolerance to self-antigens. PS can also be found on the surface of live cells and in particular tumor cells. Annexin-V (AnV) is a protein that specifically binds and blocks PS. To determine the effects of blocking PS with AnV on tumor growth and immunogenicity, mice were inoculated with AGN2a, a poorly immunogenic murine neuroblastoma that expresses high level of PS on the cell surface. Survival and anti-tumor T cell response were determined. AGN2a were engineered to secrete AnV. Secreted protein effectively blocked tumor PS. 40 % of mice inoculated with AnV-expressing AGN2a cells survived free of tumor, whereas none of the mice inoculated with control cells survived (p = 0.0062). The benefits of AnV were lost when mice were depleted of T cells. The findings suggest that AnV could protect mice from tumor challenge through an immune mediated mechanism. Mice were then immunized with irradiated AnV-secreting or control cells, and challenged with wild-type AGN2a cells. AnV-secreting cell vaccine protected 80 % of mice from AGN2a challenge, while control cell vaccine prevented tumor growth in only 30 % of animals (p = 0.012). ELISPOT analysis demonstrated that AnV-secreting cell vaccine induced a greater frequency of interferon-gamma producing splenic T cells. T cells isolated from mice immunized with AnV-secreting but not control vaccine lysed AGN2a. In summary, AnV blocked PS, enhanced T cell mediated tumor immunity, and inhibited tumor growth.
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