We are analyzing https://link.springer.com/article/10.1007/s00253-021-11307-w.

Title:
Microbial methionine transporters and biotechnological applications | Applied Microbiology and Biotechnology
Description:
Abstract Methionine (Met) is an essential amino acid with commercial value in animal feed, human nutrition, and as a chemical precursor. Microbial production of Met has seen intensive investigation towards a more sustainable alternative to the chemical synthesis that currently meets the global Met demand. Indeed, efficient Met biosynthesis has been achieved in genetically modified bacteria that harbor engineered enzymes and streamlined metabolic pathways. Very recently, the export of Met as the final step during its fermentative production has been studied and optimized, primarily through identification and expression of microbial Met efflux transporters. In this mini-review, we summarize the current knowledge on four families of Met export and import transporters that have been harnessed for the production of Met and other valuable biomolecules. These families are discussed with respect to their function, gene regulation, and biotechnological applications. We cover methods for identification and characterization of Met transporters as the basis for the further engineering of these proteins and for exploration of other solute carrier families. The available arsenal of Met transporters from different species and protein families provides blueprints not only for fermentative production but also synthetic biology systems, such as molecular sensors and cell-cell communication systems. Key points • Sustainable production of methionine (Met) using microbes is actively explored. • Met transporters of four families increase production yield and specificity. • Further applications include other biosynthetic pathways and synthetic biology.
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Keywords {🔍}

met, pubmed, article, google, scholar, cas, amino, production, protein, acid, acids, transporters, coli, transporter, methionine, engineering, central, glutamicum, biotechnol, expression, mup, export, function, family, microbial, proteins, synthetic, increase, escherichia, yjeh, families, transport, strain, metabolic, exporter, membrane, gene, corynebacterium, leue, microbiol, regulation, biology, levels, cell, brnfe, lmethionine, yield, liu, applications, bacteria,

Topics {✒️}

o-succinyl-l-homoserine-based c4-chemical production improving s-adenosyl-l-methionine production l-α-amino-n-butyric acid uv-sensitive coumarin-type fluorophore met derivative s-adenosyl-l-methionine enhanced o-succinyl-l-homoserine production s-adenosyl-methionine-dependent methyltransferases /p05069899/global-amino-acids-industry s-adenosyl-l-methionine amino acid-polyamine-organocation superfamily o-succinyl-l-homoserine o-succinyl-l-serine ubiquitin-mediated protein endocytosis branched-chain amino acids amino acid-polyamine-organocation amino acid/polyamine/organocation bacillus subtilis involved queried google scholar article download pdf producing l-amino acid branched-chained amino acids fret-based protein engineering semi-synthetic fret sensor carbonyl cyanide m-chlorophenylhydrazone potassium-selective glutamate receptor amino-acid-efflux proteins amino acid-producing bacteria synthetic signaling molecules promote cargo-specific endocytosis cell-cell communication systems arginine/agmatine antiporter adic single particle cryo-em multiple ubiquitin-dependent mechanisms transmembrane solute translocators solute carrier families specific methionine-transporting system glycyl-l-leucine peptide bacterial cell-cell communication chromophore-assisted light inactivation l-methionine feeding strategy specific l-methionine sensors harald janovjak amino acid efflux amino acid transport increased extracellular levels met overproducing metd-deficient related subjects open questions remain genetically-engineered candida yeast den hengst

Questions {❓}

And why would Met and Met transporters be the choice for such endeavor?
Some open questions remain towards this method: Is expression of a microbial membrane protein in an animal neuron possible?

Schema {🗺️}

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         headline:Microbial methionine transporters and biotechnological applications
         description:Methionine (Met) is an essential amino acid with commercial value in animal feed, human nutrition, and as a chemical precursor. Microbial production of Met has seen intensive investigation towards a more sustainable alternative to the chemical synthesis that currently meets the global Met demand. Indeed, efficient Met biosynthesis has been achieved in genetically modified bacteria that harbor engineered enzymes and streamlined metabolic pathways. Very recently, the export of Met as the final step during its fermentative production has been studied and optimized, primarily through identification and expression of microbial Met efflux transporters. In this mini-review, we summarize the current knowledge on four families of Met export and import transporters that have been harnessed for the production of Met and other valuable biomolecules. These families are discussed with respect to their function, gene regulation, and biotechnological applications. We cover methods for identification and characterization of Met transporters as the basis for the further engineering of these proteins and for exploration of other solute carrier families. The available arsenal of Met transporters from different species and protein families provides blueprints not only for fermentative production but also synthetic biology systems, such as molecular sensors and cell-cell communication systems. • Sustainable production of methionine (Met) using microbes is actively explored. • Met transporters of four families increase production yield and specificity. • Further applications include other biosynthetic pathways and synthetic biology.
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      headline:Microbial methionine transporters and biotechnological applications
      description:Methionine (Met) is an essential amino acid with commercial value in animal feed, human nutrition, and as a chemical precursor. Microbial production of Met has seen intensive investigation towards a more sustainable alternative to the chemical synthesis that currently meets the global Met demand. Indeed, efficient Met biosynthesis has been achieved in genetically modified bacteria that harbor engineered enzymes and streamlined metabolic pathways. Very recently, the export of Met as the final step during its fermentative production has been studied and optimized, primarily through identification and expression of microbial Met efflux transporters. In this mini-review, we summarize the current knowledge on four families of Met export and import transporters that have been harnessed for the production of Met and other valuable biomolecules. These families are discussed with respect to their function, gene regulation, and biotechnological applications. We cover methods for identification and characterization of Met transporters as the basis for the further engineering of these proteins and for exploration of other solute carrier families. The available arsenal of Met transporters from different species and protein families provides blueprints not only for fermentative production but also synthetic biology systems, such as molecular sensors and cell-cell communication systems. • Sustainable production of methionine (Met) using microbes is actively explored. • Met transporters of four families increase production yield and specificity. • Further applications include other biosynthetic pathways and synthetic biology.
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         Amino acids
         Solute efflux
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         Microbiology
         Microbial Genetics and Genomics
         Biotechnology
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      name:Australian Regenerative Medicine Institute (ARMI), Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia
      name:European Molecular Biology Laboratory Australia (EMBL Australia), Monash University, Clayton, Australia
      name:Australian Regenerative Medicine Institute (ARMI), Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia
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