We are analyzing https://link.springer.com/article/10.1007/s00251-003-0621-6.

Title:
Alleles carried at positions −819 and −592 of the IL10 promoter affect transcription following stimulation of peripheral blood cells with Streptococcus pneumoniae | Immunogenetics
Description:
IL-10 inhibits the production of many pro-inflammatory cytokines. Polymorphisms in the IL10 gene promoter at positions −1082G→A, −819C→T and −592C→A occur as three haplotypes, ATA, GCC and ACC. These influence several infectious and inflammatory diseases including community-acquired pneumonia, where a role for IL-10 is suggested by fluctuations in plasma levels of the cytokine. However, the effects of the haplotypes on IL-10 production are unclear. We stimulated peripheral blood mononuclear cells (PBMC) from at least five individuals homozygous for each of the three haplotypes with lipopolysaccharide (LPS, 10 μg/ml) or heat-killed Streptococcus pneumoniae (107cfu/ml) and measured IL-10 mRNA by RT-PCR. Following S. pneumoniae stimulation, PBMC with the ATA haplotype had higher IL-10 mRNA levels than those with the GCC haplotype at 4 h (independent t-test; P=0.024), or the ACC haplotype at 4 h (P<0.0001) and 8 h (P=0.007). Following LPS stimulation, IL-10 mRNA levels were not significantly influenced by the IL10 haplotype, but similar trends were observed, consistent with the variable outcome of published studies. The results suggest that the −819 and/or −592 alleles affect transcription.
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Keywords {🔍}

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Topics {✒️}

monocyte/macrophage-induced inflammatory response month download article/chapter heat-killed streptococcus pneumoniae maldi-tof mass spectrometry tumor necrosis factor-alpha article immunogenetics aims anti-inflammatory cytokine production full article pdf related subjects interleukin-10 gene promoter il10 gene promoter privacy choices/manage cookies community acquired pneumonia community-acquired pneumonia peripheral blood cells inflammatory bowel diseases lymphoma cell lines il10 promoter variants single nucleotide polymorphisms european economic area pro-inflammatory cytokines independent t-test systemic lupus erythematosus gpo box x2213 inflammatory bowel disease experimental human endotoxemia alleles carried conditions privacy policy measured il-10 mrna fc gamma receptor gram-negative bacteria inhibits cytokine synthesis il-10 mrna levels major haplotype families western australian institute medical research de straat fg article temple accepting optional cookies streptococcus pneumoniae article log pneumococcal septic shock hajeer ah royal perth hospital tnf alpha author information authors de winkel jg de vries je check access instant access

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         headline:Alleles carried at positions −819 and −592 of the IL10 promoter affect transcription following stimulation of peripheral blood cells with Streptococcus pneumoniae
         description:IL-10 inhibits the production of many pro-inflammatory cytokines. Polymorphisms in the IL10 gene promoter at positions −1082G→A, −819C→T and −592C→A occur as three haplotypes, ATA, GCC and ACC. These influence several infectious and inflammatory diseases including community-acquired pneumonia, where a role for IL-10 is suggested by fluctuations in plasma levels of the cytokine. However, the effects of the haplotypes on IL-10 production are unclear. We stimulated peripheral blood mononuclear cells (PBMC) from at least five individuals homozygous for each of the three haplotypes with lipopolysaccharide (LPS, 10 μg/ml) or heat-killed Streptococcus pneumoniae (107cfu/ml) and measured IL-10 mRNA by RT-PCR. Following S. pneumoniae stimulation, PBMC with the ATA haplotype had higher IL-10 mRNA levels than those with the GCC haplotype at 4 h (independent t-test; P=0.024), or the ACC haplotype at 4 h (P<0.0001) and 8 h (P=0.007). Following LPS stimulation, IL-10 mRNA levels were not significantly influenced by the IL10 haplotype, but similar trends were observed, consistent with the variable outcome of published studies. The results suggest that the −819 and/or −592 alleles affect transcription.
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         dateModified:2003-11-06T00:00:00Z
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            Lipopolysaccharide
             Streptococcus pneumoniae
            Promoter polymorphisms
            Immunology
            Human Genetics
            Gene Function
            Cell Biology
            Allergology
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      description:IL-10 inhibits the production of many pro-inflammatory cytokines. Polymorphisms in the IL10 gene promoter at positions −1082G→A, −819C→T and −592C→A occur as three haplotypes, ATA, GCC and ACC. These influence several infectious and inflammatory diseases including community-acquired pneumonia, where a role for IL-10 is suggested by fluctuations in plasma levels of the cytokine. However, the effects of the haplotypes on IL-10 production are unclear. We stimulated peripheral blood mononuclear cells (PBMC) from at least five individuals homozygous for each of the three haplotypes with lipopolysaccharide (LPS, 10 μg/ml) or heat-killed Streptococcus pneumoniae (107cfu/ml) and measured IL-10 mRNA by RT-PCR. Following S. pneumoniae stimulation, PBMC with the ATA haplotype had higher IL-10 mRNA levels than those with the GCC haplotype at 4 h (independent t-test; P=0.024), or the ACC haplotype at 4 h (P<0.0001) and 8 h (P=0.007). Following LPS stimulation, IL-10 mRNA levels were not significantly influenced by the IL10 haplotype, but similar trends were observed, consistent with the variable outcome of published studies. The results suggest that the −819 and/or −592 alleles affect transcription.
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         Lipopolysaccharide
          Streptococcus pneumoniae
         Promoter polymorphisms
         Immunology
         Human Genetics
         Gene Function
         Cell Biology
         Allergology
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