We are analyzing https://link.springer.com/article/10.1007/s00216-016-9605-x.

Title:
Label-free analysis of mRNA capping efficiency using RNase H probes and LC-MS | Analytical and Bioanalytical Chemistry
Description:
A label-free method for determining the 5′-end cap identity and orientation of a messenger RNA (mRNA) is described. Biotin-tagged probes that were complementary to the 5′ end of target mRNA were used with RNase H to cleave the 5′ end of the mRNA. The cleaved end sequence was isolated using streptavidin-coated magnetic beads and then analyzed by LC-MS. Quantitative and qualitative information on the 5′ cap was determined from the unique mass of the isolated cleaved sequence. This approach, combined with the use of 5′ RNA pyrophosphohydrolase, was also used to ascertain the orientation of the 5′ cap. The assay showed low-picomole sensitivity for detecting capping reaction impurities. Uncapped triphosphate mRNA, spiked into 100 pmol of capped mRNA, could be detected over the tested range of 0.5 to 25 % with a linear response. The capping efficiency of several vaccinia-capped mRNA preparations was determined to be between 88 and 98 % depending on the modification type and length of the mRNA. mRNA of 2.2K and 9K nucleotides in length and containing the modified nucleotides pseudouridine and 5-methylcytidine were all successfully analyzed, demonstrating the utility of the technique to study mRNA capping.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

The income method remains a mystery to us.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com might be making money, but it's not detectable how they're doing it.

Keywords {🔍}

article, google, scholar, cas, mrna, rna, cap, capping, rnase, analysis, synthesis, privacy, cookies, content, research, lcms, beverly, end, access, vitro, analogs, stepinski, information, publish, search, efficiency, probes, mass, modified, mrnas, shatkin, nature, cell, expression, structure, mol, stolarski, rhoads, kariko, chem, data, log, journal, labelfree, michael, dell, parmar, capped, nucleotides, chapter,

Topics {✒️}

parul parmar & leslie houghton mrna-based therapeutics—developing month download article/chapter liquid chromatography/mass spectrometry �anti-reverse” cap analogs anti-reverse cap analogs streptavidin-coated magnetic beads vaccinia-capped mrna preparations bioanalytical chemistry aims capture probe methods enzymol biotin-tagged probes privacy choices/manage cookies label-free method bmc mol biol phage rna polymerases label-free analysis full article pdf full-size enzyme synthetic modified mrna high translational efficiency rna cap synthesis vitro transcribed rna sequencing control nucleic acid vaccines mrna 5′ cap-analogues fully capped mrna cap-binding proteins study mrna capping european economic area enzymatically active xrn1 xenopus oocyte nuclei biochim biophys acta truncated cd40l protein superior translational properties sequence-dependent hydrolysis modified oligonucleotide splints strong anion-exchange highly efficient reprogramming increased translational capacity de la pena darpa grant hr-0011-13-3-0003 electronic supplementary material mrna decapping status chemically modified mrna sequence-engineered mrna mrna capping efficiency 5′-end cap identity lc-ms

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Label-free analysis of mRNA capping efficiency using RNase H probes and LC-MS
         description:A label-free method for determining the 5′-end cap identity and orientation of a messenger RNA (mRNA) is described. Biotin-tagged probes that were complementary to the 5′ end of target mRNA were used with RNase H to cleave the 5′ end of the mRNA. The cleaved end sequence was isolated using streptavidin-coated magnetic beads and then analyzed by LC-MS. Quantitative and qualitative information on the 5′ cap was determined from the unique mass of the isolated cleaved sequence. This approach, combined with the use of 5′ RNA pyrophosphohydrolase, was also used to ascertain the orientation of the 5′ cap. The assay showed low-picomole sensitivity for detecting capping reaction impurities. Uncapped triphosphate mRNA, spiked into 100 pmol of capped mRNA, could be detected over the tested range of 0.5 to 25 % with a linear response. The capping efficiency of several vaccinia-capped mRNA preparations was determined to be between 88 and 98 % depending on the modification type and length of the mRNA. mRNA of 2.2K and 9K nucleotides in length and containing the modified nucleotides pseudouridine and 5-methylcytidine were all successfully analyzed, demonstrating the utility of the technique to study mRNA capping.
         datePublished:2016-05-18T00:00:00Z
         dateModified:2016-05-18T00:00:00Z
         pageStart:5021
         pageEnd:5030
         sameAs:https://doi.org/10.1007/s00216-016-9605-x
         keywords:
            mRNA
            RNase H
            5′ cap
            LC-MS
             N7-Methylguanosine
            RNA
            Analytical Chemistry
            Biochemistry
            general
            Laboratory Medicine
            Characterization and Evaluation of Materials
            Food Science
            Monitoring/Environmental Analysis
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Figa_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig6_HTML.gif
         isPartOf:
            name:Analytical and Bioanalytical Chemistry
            issn:
               1618-2650
               1618-2642
            volumeNumber:408
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Berlin Heidelberg
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Michael Beverly
               affiliation:
                     name:Novartis Institutes of Biomedical Research
                     address:
                        name:Novartis Institutes of Biomedical Research, Cambridge, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Amy Dell
               affiliation:
                     name:Novartis Institutes of Biomedical Research
                     address:
                        name:Novartis Institutes of Biomedical Research, Cambridge, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Parul Parmar
               affiliation:
                     name:Novartis Institutes of Biomedical Research
                     address:
                        name:Novartis Institutes of Biomedical Research, Cambridge, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Leslie Houghton
               affiliation:
                     name:Novartis Institutes of Biomedical Research
                     address:
                        name:Novartis Institutes of Biomedical Research, Cambridge, USA
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Label-free analysis of mRNA capping efficiency using RNase H probes and LC-MS
      description:A label-free method for determining the 5′-end cap identity and orientation of a messenger RNA (mRNA) is described. Biotin-tagged probes that were complementary to the 5′ end of target mRNA were used with RNase H to cleave the 5′ end of the mRNA. The cleaved end sequence was isolated using streptavidin-coated magnetic beads and then analyzed by LC-MS. Quantitative and qualitative information on the 5′ cap was determined from the unique mass of the isolated cleaved sequence. This approach, combined with the use of 5′ RNA pyrophosphohydrolase, was also used to ascertain the orientation of the 5′ cap. The assay showed low-picomole sensitivity for detecting capping reaction impurities. Uncapped triphosphate mRNA, spiked into 100 pmol of capped mRNA, could be detected over the tested range of 0.5 to 25 % with a linear response. The capping efficiency of several vaccinia-capped mRNA preparations was determined to be between 88 and 98 % depending on the modification type and length of the mRNA. mRNA of 2.2K and 9K nucleotides in length and containing the modified nucleotides pseudouridine and 5-methylcytidine were all successfully analyzed, demonstrating the utility of the technique to study mRNA capping.
      datePublished:2016-05-18T00:00:00Z
      dateModified:2016-05-18T00:00:00Z
      pageStart:5021
      pageEnd:5030
      sameAs:https://doi.org/10.1007/s00216-016-9605-x
      keywords:
         mRNA
         RNase H
         5′ cap
         LC-MS
          N7-Methylguanosine
         RNA
         Analytical Chemistry
         Biochemistry
         general
         Laboratory Medicine
         Characterization and Evaluation of Materials
         Food Science
         Monitoring/Environmental Analysis
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Figa_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00216-016-9605-x/MediaObjects/216_2016_9605_Fig6_HTML.gif
      isPartOf:
         name:Analytical and Bioanalytical Chemistry
         issn:
            1618-2650
            1618-2642
         volumeNumber:408
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Berlin Heidelberg
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Michael Beverly
            affiliation:
                  name:Novartis Institutes of Biomedical Research
                  address:
                     name:Novartis Institutes of Biomedical Research, Cambridge, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Amy Dell
            affiliation:
                  name:Novartis Institutes of Biomedical Research
                  address:
                     name:Novartis Institutes of Biomedical Research, Cambridge, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Parul Parmar
            affiliation:
                  name:Novartis Institutes of Biomedical Research
                  address:
                     name:Novartis Institutes of Biomedical Research, Cambridge, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Leslie Houghton
            affiliation:
                  name:Novartis Institutes of Biomedical Research
                  address:
                     name:Novartis Institutes of Biomedical Research, Cambridge, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Analytical and Bioanalytical Chemistry
      issn:
         1618-2650
         1618-2642
      volumeNumber:408
Organization:
      name:Springer Berlin Heidelberg
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Novartis Institutes of Biomedical Research
      address:
         name:Novartis Institutes of Biomedical Research, Cambridge, USA
         type:PostalAddress
      name:Novartis Institutes of Biomedical Research
      address:
         name:Novartis Institutes of Biomedical Research, Cambridge, USA
         type:PostalAddress
      name:Novartis Institutes of Biomedical Research
      address:
         name:Novartis Institutes of Biomedical Research, Cambridge, USA
         type:PostalAddress
      name:Novartis Institutes of Biomedical Research
      address:
         name:Novartis Institutes of Biomedical Research, Cambridge, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Michael Beverly
      affiliation:
            name:Novartis Institutes of Biomedical Research
            address:
               name:Novartis Institutes of Biomedical Research, Cambridge, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Amy Dell
      affiliation:
            name:Novartis Institutes of Biomedical Research
            address:
               name:Novartis Institutes of Biomedical Research, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Parul Parmar
      affiliation:
            name:Novartis Institutes of Biomedical Research
            address:
               name:Novartis Institutes of Biomedical Research, Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Leslie Houghton
      affiliation:
            name:Novartis Institutes of Biomedical Research
            address:
               name:Novartis Institutes of Biomedical Research, Cambridge, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Novartis Institutes of Biomedical Research, Cambridge, USA
      name:Novartis Institutes of Biomedical Research, Cambridge, USA
      name:Novartis Institutes of Biomedical Research, Cambridge, USA
      name:Novartis Institutes of Biomedical Research, Cambridge, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(91)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.38s.

LINK . SPRINGER . COM {}