We are analyzing https://link.springer.com/article/10.1007/s00210-024-03387-y.

Title:
Ginsenoside Rb prevents the metastasis of hepatocarcinoma by blocking the platelet-tumor cell interaction | Naunyn-Schmiedeberg's Archives of Pharmacology
Description:
Background The interaction between platelets and tumor cells is a crucial step in the progression of tumor metastasis. Blocking platelet-tumor cell interaction is a potential target against metastasis. Ginsenoside Rb (G-Rb) exhibits potential anti-tumor pharmacological properties and may offer a therapeutic option for cancer. Purpose This study aimed to investigate anti-metastatic effects of G-Rb through regulating the crosstalk of platelets with tumor cells. Methods In order to explore anti-metastatic effects of G-Rb in vitro, HepG2 cell and platelets were co-cultured to mimic the interaction of platelets with tumor cells. Wound healing and Transwell assays were used to assess the effect of G-Rb on cell migration and invasion. The expression of epithelial-mesenchymal transition (EMT)–related markers was determined by RT-qPCR and western blot assays. The aggregation and activation of platelets were detected by flow cytometry. Moreover, a lung metastasis model of mice was established to evaluate inhibitory effects of G-Rb in vivo. Metastatic nodules on the lung surface were counted and sections of lung tissues were stained by H&E. Results G-Rb effectively suppressed tumor metastasis in the co-culture of platelets with HepG2 cell. First, G-Rb treatment significantly inhibited the migration and invasion of HepG2 cells induced by platelets. Second, the expressions of EMT-related markers, including N-cadherin, Snail, and MMP9, were decreased by the treatment of G-Rb in the presence of platelets. Meanwhile, G-Rb also suppressed platelet hyperactivity by regulating the adhesion to tumor cells, activation, TCIPA, and TGF-β1 secretion of platelets in vitro. In addition, the results of in vivo experiments proved G-Rb administration not only significantly decreased lung metastasis but also attenuated platelets aberrant aggregation and activation in vivo. Conclusion Our findings showed that G-Rb inhibited tumor metastasis and platelet activation through mediating platelet-tumor cell interaction, indicating the potential values of G-Rb in tumor metastasis therapy.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Telecommunications
Science

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,016 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

The income method remains a mystery to us.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com might be cashing in, but we can't detect the method they're using.

Keywords {🔍}

pubmed, article, google, scholar, platelets, cancer, tumor, metastasis, cell, central, grb, cells, data, interaction, yang, platelet, chen, activation, platelettumor, access, shandong, supplementary, privacy, cookies, content, information, ginsenoside, miao, han, lung, res, china, springer, publish, research, search, prevents, longxing, cheng, lijing, chunchao, progression, potential, transition, aggregation, mice, clin, nat, rev, zhang,

Topics {✒️}

tumor-cell-induced platelet aggregation month download article/chapter explore anti-metastatic effects wnt-β-catenin pathway investigate anti-metastatic effects tumor-supportive macroenvironment defined platelet-tumor cell interaction bone marrow-derived cells article naunyn-schmiedeberg' suppressed platelet hyperactivity full article pdf privacy choices/manage cookies tumor-platelet communications g-rb access inhibiting cell proliferation ginsenoside rb prevents pro-metastatic support luminal breast cancers related subjects early metastatic niches tumor-platelet adhesion tumor metastasis therapy tumor cells behavior murine breast cancer accepted manuscript version promoting platelet apoptosis hepg2 cells induced epithelial-mesenchymal transition evaluate inhibitory effects including n-cadherin protein-coupled receptors lung metastasis model holds exclusive rights shear induced damage european economic area tgf-β1 secretion alleviates oxidative stress c-type lectin emerging biological principles de miguel-perez ethyl-acetate extract spatholobi caulis blocked ethics declarations conflict emt-related markers chunchao han conditions privacy policy shandong university foundation article miao western blot assays cancer cells induces

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Ginsenoside Rb prevents the metastasis of hepatocarcinoma by blocking the platelet-tumor cell interaction
         description:The interaction between platelets and tumor cells is a crucial step in the progression of tumor metastasis. Blocking platelet-tumor cell interaction is a potential target against metastasis. Ginsenoside Rb (G-Rb) exhibits potential anti-tumor pharmacological properties and may offer a therapeutic option for cancer. This study aimed to investigate anti-metastatic effects of G-Rb through regulating the crosstalk of platelets with tumor cells. In order to explore anti-metastatic effects of G-Rb in vitro, HepG2 cell and platelets were co-cultured to mimic the interaction of platelets with tumor cells. Wound healing and Transwell assays were used to assess the effect of G-Rb on cell migration and invasion. The expression of epithelial-mesenchymal transition (EMT)–related markers was determined by RT-qPCR and western blot assays. The aggregation and activation of platelets were detected by flow cytometry. Moreover, a lung metastasis model of mice was established to evaluate inhibitory effects of G-Rb in vivo. Metastatic nodules on the lung surface were counted and sections of lung tissues were stained by H&E. G-Rb effectively suppressed tumor metastasis in the co-culture of platelets with HepG2 cell. First, G-Rb treatment significantly inhibited the migration and invasion of HepG2 cells induced by platelets. Second, the expressions of EMT-related markers, including N-cadherin, Snail, and MMP9, were decreased by the treatment of G-Rb in the presence of platelets. Meanwhile, G-Rb also suppressed platelet hyperactivity by regulating the adhesion to tumor cells, activation, TCIPA, and TGF-β1 secretion of platelets in vitro. In addition, the results of in vivo experiments proved G-Rb administration not only significantly decreased lung metastasis but also attenuated platelets aberrant aggregation and activation in vivo. Our findings showed that G-Rb inhibited tumor metastasis and platelet activation through mediating platelet-tumor cell interaction, indicating the potential values of G-Rb in tumor metastasis therapy.
         datePublished:2024-08-22T00:00:00Z
         dateModified:2024-08-22T00:00:00Z
         pageStart:1721
         pageEnd:1733
         sameAs:https://doi.org/10.1007/s00210-024-03387-y
         keywords:
            Platelet-tumor cell interaction; Tumor metastasis; EMT; G-Rb
            Pharmacology/Toxicology
            Neurosciences
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig4_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig5_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig6_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig7_HTML.png
         isPartOf:
            name:Naunyn-Schmiedeberg's Archives of Pharmacology
            issn:
               1432-1912
               0028-1298
            volumeNumber:398
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Berlin Heidelberg
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Longxing Miao
               affiliation:
                     name:Shandong University of Traditional Chinese Medicine
                     address:
                        name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
                     name:The Second Hospital, Cheeloo College of Medicine, Shandong University
                     address:
                        name:The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Yijun Yang
               affiliation:
                     name:Shandong Medical College
                     address:
                        name:Department of Pharmacy, Shandong Medical College, Jinan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Mengtao Cheng
               affiliation:
                     name:Shandong University of Traditional Chinese Medicine
                     address:
                        name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Lijing Chen
               affiliation:
                     name:Shandong University of Traditional Chinese Medicine
                     address:
                        name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
                     name:University of Traditional Chinese Medicine
                     address:
                        name:The Second Affiliated Hospital of Shandong, University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Chunchao Han
               affiliation:
                     name:Shandong University of Traditional Chinese Medicine
                     address:
                        name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Ginsenoside Rb prevents the metastasis of hepatocarcinoma by blocking the platelet-tumor cell interaction
      description:The interaction between platelets and tumor cells is a crucial step in the progression of tumor metastasis. Blocking platelet-tumor cell interaction is a potential target against metastasis. Ginsenoside Rb (G-Rb) exhibits potential anti-tumor pharmacological properties and may offer a therapeutic option for cancer. This study aimed to investigate anti-metastatic effects of G-Rb through regulating the crosstalk of platelets with tumor cells. In order to explore anti-metastatic effects of G-Rb in vitro, HepG2 cell and platelets were co-cultured to mimic the interaction of platelets with tumor cells. Wound healing and Transwell assays were used to assess the effect of G-Rb on cell migration and invasion. The expression of epithelial-mesenchymal transition (EMT)–related markers was determined by RT-qPCR and western blot assays. The aggregation and activation of platelets were detected by flow cytometry. Moreover, a lung metastasis model of mice was established to evaluate inhibitory effects of G-Rb in vivo. Metastatic nodules on the lung surface were counted and sections of lung tissues were stained by H&E. G-Rb effectively suppressed tumor metastasis in the co-culture of platelets with HepG2 cell. First, G-Rb treatment significantly inhibited the migration and invasion of HepG2 cells induced by platelets. Second, the expressions of EMT-related markers, including N-cadherin, Snail, and MMP9, were decreased by the treatment of G-Rb in the presence of platelets. Meanwhile, G-Rb also suppressed platelet hyperactivity by regulating the adhesion to tumor cells, activation, TCIPA, and TGF-β1 secretion of platelets in vitro. In addition, the results of in vivo experiments proved G-Rb administration not only significantly decreased lung metastasis but also attenuated platelets aberrant aggregation and activation in vivo. Our findings showed that G-Rb inhibited tumor metastasis and platelet activation through mediating platelet-tumor cell interaction, indicating the potential values of G-Rb in tumor metastasis therapy.
      datePublished:2024-08-22T00:00:00Z
      dateModified:2024-08-22T00:00:00Z
      pageStart:1721
      pageEnd:1733
      sameAs:https://doi.org/10.1007/s00210-024-03387-y
      keywords:
         Platelet-tumor cell interaction; Tumor metastasis; EMT; G-Rb
         Pharmacology/Toxicology
         Neurosciences
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig4_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig5_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig6_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00210-024-03387-y/MediaObjects/210_2024_3387_Fig7_HTML.png
      isPartOf:
         name:Naunyn-Schmiedeberg's Archives of Pharmacology
         issn:
            1432-1912
            0028-1298
         volumeNumber:398
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Berlin Heidelberg
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Longxing Miao
            affiliation:
                  name:Shandong University of Traditional Chinese Medicine
                  address:
                     name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
                  name:The Second Hospital, Cheeloo College of Medicine, Shandong University
                  address:
                     name:The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yijun Yang
            affiliation:
                  name:Shandong Medical College
                  address:
                     name:Department of Pharmacy, Shandong Medical College, Jinan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mengtao Cheng
            affiliation:
                  name:Shandong University of Traditional Chinese Medicine
                  address:
                     name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Lijing Chen
            affiliation:
                  name:Shandong University of Traditional Chinese Medicine
                  address:
                     name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
                  name:University of Traditional Chinese Medicine
                  address:
                     name:The Second Affiliated Hospital of Shandong, University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Chunchao Han
            affiliation:
                  name:Shandong University of Traditional Chinese Medicine
                  address:
                     name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Naunyn-Schmiedeberg's Archives of Pharmacology
      issn:
         1432-1912
         0028-1298
      volumeNumber:398
Organization:
      name:Springer Berlin Heidelberg
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Shandong University of Traditional Chinese Medicine
      address:
         name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
         type:PostalAddress
      name:The Second Hospital, Cheeloo College of Medicine, Shandong University
      address:
         name:The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of China
         type:PostalAddress
      name:Shandong Medical College
      address:
         name:Department of Pharmacy, Shandong Medical College, Jinan, People’s Republic of China
         type:PostalAddress
      name:Shandong University of Traditional Chinese Medicine
      address:
         name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
         type:PostalAddress
      name:Shandong University of Traditional Chinese Medicine
      address:
         name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
         type:PostalAddress
      name:University of Traditional Chinese Medicine
      address:
         name:The Second Affiliated Hospital of Shandong, University of Traditional Chinese Medicine, Jinan, People’s Republic of China
         type:PostalAddress
      name:Shandong University of Traditional Chinese Medicine
      address:
         name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Longxing Miao
      affiliation:
            name:Shandong University of Traditional Chinese Medicine
            address:
               name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
               type:PostalAddress
            type:Organization
            name:The Second Hospital, Cheeloo College of Medicine, Shandong University
            address:
               name:The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Yijun Yang
      affiliation:
            name:Shandong Medical College
            address:
               name:Department of Pharmacy, Shandong Medical College, Jinan, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Mengtao Cheng
      affiliation:
            name:Shandong University of Traditional Chinese Medicine
            address:
               name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Lijing Chen
      affiliation:
            name:Shandong University of Traditional Chinese Medicine
            address:
               name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
               type:PostalAddress
            type:Organization
            name:University of Traditional Chinese Medicine
            address:
               name:The Second Affiliated Hospital of Shandong, University of Traditional Chinese Medicine, Jinan, People’s Republic of China
               type:PostalAddress
            type:Organization
      name:Chunchao Han
      affiliation:
            name:Shandong University of Traditional Chinese Medicine
            address:
               name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
      name:The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People’s Republic of China
      name:Department of Pharmacy, Shandong Medical College, Jinan, People’s Republic of China
      name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
      name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
      name:The Second Affiliated Hospital of Shandong, University of Traditional Chinese Medicine, Jinan, People’s Republic of China
      name:School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, People’s Republic of China
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(141)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.49s.

LINK . SPRINGER . COM {}