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LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. External Links
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We are analyzing https://link.springer.com/article/10.1007/s00018-025-05651-7.

Title:
circCEP70 encoded protein inhibits the progression of hepatocellular carcinoma | Cellular and Molecular Life Sciences
Description:
Cirrhosis is closely related to hepatocellular carcinoma (HCC), however, the regulation of circular RNA (circRNA) in HCC with cirrhotic background has not yet been well illustrated. In this study, high throughput circRNA sequencing was applied to identified candidate circRNAs in HCC samples with cirrhotic background. The biological function of candidate circRNA was validated in both in vitro and in vivo settings. Additionally, Alphafold 3, mass spectrometry analysis and immunofluorescence were employed to investigate the underlying mechanisms involved. We found circCEP70 exhibited significantly higher expression levels in cirrhotic HCC samples and showed a positive correlation with improved prognosis. The RNA binding protein U2AF2 was found to suppress the expression of circCEP70 in cirrhosis patients. In vitro and in vivo experiments, including CCK-8, EdU, plate cloning, transwell, scratch, subcutaneous tumor formation, liver metastasis in situ, and lung metastasis assays confirmed the anti-carcinogenic effects. Mechanistically, circCEP70 encoded a novel protein named CEP70-160aa, which interacted with PKM2 and hindered its translocation into the nucleus. This interaction led to reduce STAT3 phosphorylation in the nucleus, thus inhibiting HCC proliferation and metastasis. In cirrhotic microenvironment, circCEP70 prevented HCC proliferation and metastasis through PKM2/STAT3 axis, and RNA binding protein U2AF2 could inhibit circCEP70 expression.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {šŸ“š}

  • Science
  • Education
  • Health & Fitness

Content Management System {šŸ“}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {šŸ“ˆ}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
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How Does Link.springer.com Make Money? {šŸ’ø}

We find it hard to spot revenue streams.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Link.springer.com could be getting rich in stealth mode, or the way it's monetizing isn't detectable.

Keywords {šŸ”}

circcep, pubmed, hcc, article, cepaa, fig, google, scholar, pkm, protein, cell, cas, expression, rna, cells, tumor, china, circular, central, cancer, uaf, stat, zhang, carcinoma, wang, metastasis, hepatocellular, study, experiments, liver, samples, performed, figure, cirrhotic, nucleus, proliferation, overexpression, data, patients, proteins, sequence, research, downstream, nuclear, analysis, phosphorylation, yang, circrnas, showed, binding,

Topics {āœ’ļø}

circ-sh3rf3/gata-4/mir-29a regulatory axis article download pdf caf-derived tumor suppressor post-translational modifications compared dual-fluorescent reporter assay amino-benzidine-based staining method light/dark cycle suppressing mek1/erk signaling p-stat3/il17a axis dual-fluorescent enzyme assay protein-nucleic acid interactions hepato-pancreato-biliary surgery high-affinity binding sites forecasting protein–ligand interactions recurrence rate ensure specific pathogen-free conditions dual-fluorescent protein plasmid mir-607/crisp3 axis rna-binding proteins characterizing circrna-protein interaction mutated cep70-160aa promoter fine-tuning stat3 activation open reading frame small-molecule compound tepp-46 x-axis represented flag-tagged cep70-160aa protein–protein interaction prediction wild-type ires sequence tumor suppressor function emt-driven metastasis regulation du ww sk-hep-1 cells ranging protein–protein interaction studies plasmid overexpressing cep70-160aa protein named cep70-160aa full size image cep70-160aa protein complex nuclear/cytoplasmic fractionation results hsa_circ_0003998 promotes epithelial promotes malignant behaviors benzene-induced hematotoxicity aggressive proliferative capacity 2Ā months post-procedure privacy choices/manage cookies enhancing immune surveillance custom-made commercial antibody related subjects dual-luciferase assay cep70-160aa customized antibody cirrhotic hepatocellular carcinoma

Questions {ā“}

  • Cogliati B, Yashaswini CN, Wang S, Sia D, Friedman SL (2023) Friend or foe?

Schema {šŸ—ŗļø}

WebPage:
      mainEntity:
         headline:circCEP70 encoded protein inhibits the progression of hepatocellular carcinoma
         description:Cirrhosis is closely related to hepatocellular carcinoma (HCC), however, the regulation of circular RNA (circRNA) in HCC with cirrhotic background has not yet been well illustrated. In this study, high throughput circRNA sequencing was applied to identified candidate circRNAs in HCC samples with cirrhotic background. The biological function of candidate circRNA was validated in both in vitro and in vivo settings. Additionally, Alphafold 3, mass spectrometry analysis and immunofluorescence were employed to investigate the underlying mechanisms involved. We found circCEP70 exhibited significantly higher expression levels in cirrhotic HCC samples and showed a positive correlation with improved prognosis. The RNA binding protein U2AF2 was found to suppress the expression of circCEP70 in cirrhosis patients. In vitro and in vivo experiments, including CCK-8, EdU, plate cloning, transwell, scratch, subcutaneous tumor formation, liver metastasis in situ, and lung metastasis assays confirmed the anti-carcinogenic effects. Mechanistically, circCEP70 encoded a novel protein named CEP70-160aa, which interacted with PKM2 and hindered its translocation into the nucleus. This interaction led to reduce STAT3 phosphorylation in the nucleus, thus inhibiting HCC proliferation and metastasis. In cirrhotic microenvironment, circCEP70 prevented HCC proliferation and metastasis through PKM2/STAT3 axis, and RNA binding protein U2AF2 could inhibit circCEP70 expression.
         datePublished:2025-04-24T00:00:00Z
         dateModified:2025-04-24T00:00:00Z
         pageStart:1
         pageEnd:19
         license:http://creativecommons.org/licenses/by-nc-nd/4.0/
         sameAs:https://doi.org/10.1007/s00018-025-05651-7
         keywords:
            Hepatocellular carcinoma
            CircCEP70
            Cirrhosis
            Alphafold 3
            Cell Biology
            Biomedicine
            general
            Life Sciences
            Biochemistry
         image:
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            issn:
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         author:
               name:Lian Li
               affiliation:
                     name:Sichuan University
                     address:
                        name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                        type:PostalAddress
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                     address:
                        name:Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, China
                        type:PostalAddress
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                     name:Sichuan University
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                        name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                        type:PostalAddress
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                     name:Jinan University (Shenzhen People’s Hospital)
                     address:
                        name:Department of Thoracic Surgery, the Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital), Shenzhen, China
                        type:PostalAddress
                     type:Organization
                     name:Jinan University
                     address:
                        name:The First Affiliated Hospital, Jinan University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Peng Wang
               affiliation:
                     name:University of Electronic Science and Technology of China
                     address:
                        name:Department of Burns, Sichuan Academy of Medical Science, Sichuan Provincial People’S Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Mingqing Xu
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                     address:
                        name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                        type:PostalAddress
                     type:Organization
                     name:Sichuan University
                     address:
                        name:Department of Hepato-Pancreato-Biliary Surgery, Meishan City People’S Hospital, Meishan Hospital of West China Hospital, Sichuan University, Meishan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Bo Li
               affiliation:
                     name:Sichuan University
                     address:
                        name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ming Zhang
               url:http://orcid.org/0000-0002-8276-691X
               affiliation:
                     name:Sichuan University
                     address:
                        name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                        type:PostalAddress
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               email:[email protected]
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      context:https://schema.org
ScholarlyArticle:
      headline:circCEP70 encoded protein inhibits the progression of hepatocellular carcinoma
      description:Cirrhosis is closely related to hepatocellular carcinoma (HCC), however, the regulation of circular RNA (circRNA) in HCC with cirrhotic background has not yet been well illustrated. In this study, high throughput circRNA sequencing was applied to identified candidate circRNAs in HCC samples with cirrhotic background. The biological function of candidate circRNA was validated in both in vitro and in vivo settings. Additionally, Alphafold 3, mass spectrometry analysis and immunofluorescence were employed to investigate the underlying mechanisms involved. We found circCEP70 exhibited significantly higher expression levels in cirrhotic HCC samples and showed a positive correlation with improved prognosis. The RNA binding protein U2AF2 was found to suppress the expression of circCEP70 in cirrhosis patients. In vitro and in vivo experiments, including CCK-8, EdU, plate cloning, transwell, scratch, subcutaneous tumor formation, liver metastasis in situ, and lung metastasis assays confirmed the anti-carcinogenic effects. Mechanistically, circCEP70 encoded a novel protein named CEP70-160aa, which interacted with PKM2 and hindered its translocation into the nucleus. This interaction led to reduce STAT3 phosphorylation in the nucleus, thus inhibiting HCC proliferation and metastasis. In cirrhotic microenvironment, circCEP70 prevented HCC proliferation and metastasis through PKM2/STAT3 axis, and RNA binding protein U2AF2 could inhibit circCEP70 expression.
      datePublished:2025-04-24T00:00:00Z
      dateModified:2025-04-24T00:00:00Z
      pageStart:1
      pageEnd:19
      license:http://creativecommons.org/licenses/by-nc-nd/4.0/
      sameAs:https://doi.org/10.1007/s00018-025-05651-7
      keywords:
         Hepatocellular carcinoma
         CircCEP70
         Cirrhosis
         Alphafold 3
         Cell Biology
         Biomedicine
         general
         Life Sciences
         Biochemistry
      image:
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      isPartOf:
         name:Cellular and Molecular Life Sciences
         issn:
            1420-9071
         volumeNumber:82
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer International Publishing
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Lian Li
            affiliation:
                  name:Sichuan University
                  address:
                     name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                     type:PostalAddress
                  type:Organization
                  name:Sichuan University
                  address:
                     name:Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Liangliang Xu
            affiliation:
                  name:Sichuan University
                  address:
                     name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Wenwei Liao
            affiliation:
                  name:Jinan University (Shenzhen People’s Hospital)
                  address:
                     name:Department of Thoracic Surgery, the Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital), Shenzhen, China
                     type:PostalAddress
                  type:Organization
                  name:Jinan University
                  address:
                     name:The First Affiliated Hospital, Jinan University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Peng Wang
            affiliation:
                  name:University of Electronic Science and Technology of China
                  address:
                     name:Department of Burns, Sichuan Academy of Medical Science, Sichuan Provincial People’S Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mingqing Xu
            affiliation:
                  name:Sichuan University
                  address:
                     name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                     type:PostalAddress
                  type:Organization
                  name:Sichuan University
                  address:
                     name:Department of Hepato-Pancreato-Biliary Surgery, Meishan City People’S Hospital, Meishan Hospital of West China Hospital, Sichuan University, Meishan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Bo Li
            affiliation:
                  name:Sichuan University
                  address:
                     name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ming Zhang
            url:http://orcid.org/0000-0002-8276-691X
            affiliation:
                  name:Sichuan University
                  address:
                     name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
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["Periodical","PublicationVolume"]:
      name:Cellular and Molecular Life Sciences
      issn:
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      name:Springer International Publishing
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      address:
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      address:
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         type:PostalAddress
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         name:Department of Thoracic Surgery, the Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital), Shenzhen, China
         type:PostalAddress
      name:Jinan University
      address:
         name:The First Affiliated Hospital, Jinan University, Guangzhou, China
         type:PostalAddress
      name:University of Electronic Science and Technology of China
      address:
         name:Department of Burns, Sichuan Academy of Medical Science, Sichuan Provincial People’S Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China
         type:PostalAddress
      name:Sichuan University
      address:
         name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
         type:PostalAddress
      name:Sichuan University
      address:
         name:Department of Hepato-Pancreato-Biliary Surgery, Meishan City People’S Hospital, Meishan Hospital of West China Hospital, Sichuan University, Meishan, China
         type:PostalAddress
      name:Sichuan University
      address:
         name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
         type:PostalAddress
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      address:
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Person:
      name:Lian Li
      affiliation:
            name:Sichuan University
            address:
               name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
               type:PostalAddress
            type:Organization
            name:Sichuan University
            address:
               name:Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, China
               type:PostalAddress
            type:Organization
      name:Liangliang Xu
      affiliation:
            name:Sichuan University
            address:
               name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
               type:PostalAddress
            type:Organization
      name:Wenwei Liao
      affiliation:
            name:Jinan University (Shenzhen People’s Hospital)
            address:
               name:Department of Thoracic Surgery, the Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital), Shenzhen, China
               type:PostalAddress
            type:Organization
            name:Jinan University
            address:
               name:The First Affiliated Hospital, Jinan University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Peng Wang
      affiliation:
            name:University of Electronic Science and Technology of China
            address:
               name:Department of Burns, Sichuan Academy of Medical Science, Sichuan Provincial People’S Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China
               type:PostalAddress
            type:Organization
      name:Mingqing Xu
      affiliation:
            name:Sichuan University
            address:
               name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
               type:PostalAddress
            type:Organization
            name:Sichuan University
            address:
               name:Department of Hepato-Pancreato-Biliary Surgery, Meishan City People’S Hospital, Meishan Hospital of West China Hospital, Sichuan University, Meishan, China
               type:PostalAddress
            type:Organization
      name:Bo Li
      affiliation:
            name:Sichuan University
            address:
               name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
               type:PostalAddress
            type:Organization
      name:Ming Zhang
      url:http://orcid.org/0000-0002-8276-691X
      affiliation:
            name:Sichuan University
            address:
               name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
      name:Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, China
      name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
      name:Department of Thoracic Surgery, the Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital), Shenzhen, China
      name:The First Affiliated Hospital, Jinan University, Guangzhou, China
      name:Department of Burns, Sichuan Academy of Medical Science, Sichuan Provincial People’S Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China
      name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
      name:Department of Hepato-Pancreato-Biliary Surgery, Meishan City People’S Hospital, Meishan Hospital of West China Hospital, Sichuan University, Meishan, China
      name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China
      name:Division of Liver Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China

External Links {šŸ”—}(221)

Analytics and Tracking {šŸ“Š}

  • Google Tag Manager

Libraries {šŸ“š}

  • Clipboard.js
  • Prism.js

CDN Services {šŸ“¦}

  • Crossref

5.09s.