We are analyzing https://link.springer.com/article/10.1007/s00018-024-05552-1.

Title:
Dnajc5b contributes to male fertility by maintaining the mitochondrial functions and autophagic homeostasis during spermiogenesis | Cellular and Molecular Life Sciences
Description:
DnaJ heat shock protein family member C5 beta (DNAJC5B), also known as cysteine-string protein beta, exhibits a prominent expression in testicular tissue and plays an important role in acrosomal exocytosis in vitro. Nevertheless, the precise role and underlying mechanism of DNAJC5B in spermatogenesis and male fertility remain poorly understood. The meta-analysis of RNA-sequencing datasets from porcine and murine testes reveals that Dnajc5b could be a pivotal factor in spermatogenesis. This study illustrates that male fertility declines with an increased ratio of abnormal spermatozoa in germ-cell knockout Dnajc5b mice. DNAJC5B has been identified as a mitochondrial protein with high expression in spermatids. The absence of DNAJC5B induces a cascade of mitochondrial damages, including oxidative stress, mitochondrial stress in the testes, and lower mitochondrial membrane potential of spermatozoa. In vivo and in vitro evidence demonstrates that DNAJC5B mitigates excessive cellular autophagy and mitophagy via DNAJ domain under environmental stress conditions, such as starvation or exposure to mitochondrial uncouplers FCCP and CCCP. This study highlights the important role of DNAJC5B in safeguarding male fertility by preserving mitochondrial function and regulating autophagy during spermiogenesis.
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Keywords {🔍}

dnajcb, mitochondrial, fig, cells, cko, spermatozoa, protein, autophagy, article, mitochondria, mice, genes, control, google, scholar, proteins, spermatogenesis, cell, cas, spermatids, testes, male, membrane, data, metaanalysis, stress, gene, murine, sperm, supplementary, blot, levels, function, analysis, testis, western, cellular, homeostasis, datasets, samples, fertility, spermiogenesis, zhang, autophagic, mitophagy, values, testicular, acrosome, role, porcine,

Topics {✒️}

pcmv-t7-mcs-3×flag-wpre-neo bnip3/bnip3l-dependent excessive mitophagy high-throughput rna-seq strategy meta-analyzing rna-seq data lc3b-ii + cq/α-tubulin lc3b-ii-cq/α-tubulin jialian li & fenge li cysteine-string protein beta signaling-mediated cysteine oxidation article download pdf full-length dnajc5b vector full-length dnajc5b plasmid lc3b-ii significantly decreased germ cell-knockout dnajc5b facilitating misfold-protein clearance anti-fade mounting medium exploiting rna-sequencing data hur-mediated excessive apoptosis lc3b-ii + cq/gapdh lc3b-ii-cq/gapdh acyl–biotin exchange assay acyl-biotin exchange assay called cysteine-string proteins specific pathogen-free facility acyl-biotin exchange method single-end rna reads germ-cell mitochondrial homeostasis rna-seq datasets fluoride-induced sperm damage wild-type female mice genome-wide differential expression quantitative real-time pcr pre-cold wash buffer genome-wide data mining dnajc5 family members primers cre-pf/pr mito-tracker red cmxros mitochondrial stress-related genes sperm-conditional knockout crossing dnajc5bflox/flox mice cys-domain deleted vectors significantly elevated proportion privacy choices/manage cookies rna-sequencing datasets amino acids starvation dnajc5b-flox mouse model cysteine string protein acyl-biotin exchange integrated single cell large-scale meta-analysis

Schema {🗺️}

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         description:DnaJ heat shock protein family member C5 beta (DNAJC5B), also known as cysteine-string protein beta, exhibits a prominent expression in testicular tissue and plays an important role in acrosomal exocytosis in vitro. Nevertheless, the precise role and underlying mechanism of DNAJC5B in spermatogenesis and male fertility remain poorly understood. The meta-analysis of RNA-sequencing datasets from porcine and murine testes reveals that Dnajc5b could be a pivotal factor in spermatogenesis. This study illustrates that male fertility declines with an increased ratio of abnormal spermatozoa in germ-cell knockout Dnajc5b mice. DNAJC5B has been identified as a mitochondrial protein with high expression in spermatids. The absence of DNAJC5B induces a cascade of mitochondrial damages, including oxidative stress, mitochondrial stress in the testes, and lower mitochondrial membrane potential of spermatozoa. In vivo and in vitro evidence demonstrates that DNAJC5B mitigates excessive cellular autophagy and mitophagy via DNAJ domain under environmental stress conditions, such as starvation or exposure to mitochondrial uncouplers FCCP and CCCP. This study highlights the important role of DNAJC5B in safeguarding male fertility by preserving mitochondrial function and regulating autophagy during spermiogenesis.
         datePublished:2025-02-03T00:00:00Z
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      headline:Dnajc5b contributes to male fertility by maintaining the mitochondrial functions and autophagic homeostasis during spermiogenesis
      description:DnaJ heat shock protein family member C5 beta (DNAJC5B), also known as cysteine-string protein beta, exhibits a prominent expression in testicular tissue and plays an important role in acrosomal exocytosis in vitro. Nevertheless, the precise role and underlying mechanism of DNAJC5B in spermatogenesis and male fertility remain poorly understood. The meta-analysis of RNA-sequencing datasets from porcine and murine testes reveals that Dnajc5b could be a pivotal factor in spermatogenesis. This study illustrates that male fertility declines with an increased ratio of abnormal spermatozoa in germ-cell knockout Dnajc5b mice. DNAJC5B has been identified as a mitochondrial protein with high expression in spermatids. The absence of DNAJC5B induces a cascade of mitochondrial damages, including oxidative stress, mitochondrial stress in the testes, and lower mitochondrial membrane potential of spermatozoa. In vivo and in vitro evidence demonstrates that DNAJC5B mitigates excessive cellular autophagy and mitophagy via DNAJ domain under environmental stress conditions, such as starvation or exposure to mitochondrial uncouplers FCCP and CCCP. This study highlights the important role of DNAJC5B in safeguarding male fertility by preserving mitochondrial function and regulating autophagy during spermiogenesis.
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         Autophagy
         Cell Biology
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         Biochemistry
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               type:PostalAddress
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PostalAddress:
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      name:Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, People’s Republic of China
      name:The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, People’s Republic of China

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