We are analyzing https://link.springer.com/article/10.1007/bf02619616.

Title:
Calcium regulation of normal human mammary epithelial cell growth in culture | In Vitro Cellular & Developmental Biology - Plant
Description:
The concentration of Ca++ in culture media profoundly affected the growth and differentiation properties of normal human mammary epithelial cells in short-term culture. In media where Ca++ was above 0.06 mM, longevity was limited to an average of three to four cell divisions. The extended growth fraction (those cells able, to divide more than once) was only approximately 50% and diminished to zero quickly with time. Stationary cells inhibited from dividing appeared differentiated in the formation of lipid vacuoles and accumulation of α-lactalbumin. Growth of stationary cultures could be reinstituted in about half the cells, either by disruption and transfer or by a reduction in Ca++ to less than 0.08 mM. The reduction of Ca++ to levels below 0.08 mM extended the longevity of normal cells to eight to nine divisions. The extended growth fraction was 100%. Under these conditions, cells did not differentiate. The effects of Ca++ on growth and differentiation were specific (Mg++ and Mn++ variations were without effect) and reversible and in many respects resembled Ca++ effects on epidermal cells. One major difference is that the dual pathways of growth and differentiation in mammary cells were controlled by glucocorticoid and insulin. Based on the kinetics of the reversible Ca++-induced coupling and uncoupling of proliferation and the program of differentiation, we propose that Ca++ may be an essential trigger for cell divisions that commit a mammary cell to differentiate progressively in a permissive hormonal milieu.
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Keywords {🔍}

google, scholar, cells, growth, article, cell, cas, mammary, pubmed, cancer, culture, epithelial, human, calcium, differentiation, vitro, normal, res, natl, privacy, cookies, content, mcgrath, proliferation, access, mouse, transformation, medium, chemical, carcinogenesis, proc, usa, information, publish, research, search, regulation, soule, hormones, acad, sci, media, data, log, journal, divisions, extended, effects, epidermal, insulin,

Topics {✒️}

month download article/chapter l-type calcium channels calcium-binding protein regucalcin carcinogen-induced phenotypic alterations chemical carcinogen-treated mice reversible ca++-induced coupling human epithelial cells virus-transformed cells mammary epithelial cells human mammary epithelium full article pdf privacy choices/manage cookies human wi-38 proliferation mouse mammary epithelium carcinogen altered differentiation mammary cell short-term culture tissue culture medium collagen gel culture cell culture mouse epidermal cells european economic area dividing appeared differentiated permissive hormonal milieu 5α-dihydrotestosterone regulates high-density lipoprotein government printing office balb/ccrgl mice calciumchelation-induced disruption anthracene orn-nitrosomethylurea hormonally defined medium grants nih-ca18175 stationary cells inhibited collagen gel surfaces chemical rubber company serum-free medium tumor-producing capabilities multiplication-stimulating activity extended growth fraction extracellular calcium cell populations fibroblast growth factor accepting optional cookies related subjects calcium regulation breast cancer res conditions privacy policy epithelial cells main content log cell biology

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         description:The concentration of Ca++ in culture media profoundly affected the growth and differentiation properties of normal human mammary epithelial cells in short-term culture. In media where Ca++ was above 0.06 mM, longevity was limited to an average of three to four cell divisions. The extended growth fraction (those cells able, to divide more than once) was only approximately 50% and diminished to zero quickly with time. Stationary cells inhibited from dividing appeared differentiated in the formation of lipid vacuoles and accumulation of α-lactalbumin. Growth of stationary cultures could be reinstituted in about half the cells, either by disruption and transfer or by a reduction in Ca++ to less than 0.08 mM. The reduction of Ca++ to levels below 0.08 mM extended the longevity of normal cells to eight to nine divisions. The extended growth fraction was 100%. Under these conditions, cells did not differentiate. The effects of Ca++ on growth and differentiation were specific (Mg++ and Mn++ variations were without effect) and reversible and in many respects resembled Ca++ effects on epidermal cells. One major difference is that the dual pathways of growth and differentiation in mammary cells were controlled by glucocorticoid and insulin. Based on the kinetics of the reversible Ca++-induced coupling and uncoupling of proliferation and the program of differentiation, we propose that Ca++ may be an essential trigger for cell divisions that commit a mammary cell to differentiate progressively in a permissive hormonal milieu.
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