We are analyzing https://link.springer.com/article/10.1007/bf02618365.

Title:
Growth of normal human mammary cells in culture | In Vitro Cellular & Developmental Biology - Plant
Description:
Reduction mammoplasty tissue was used to obtain short-term cultures of human epithelial cell populations. Digestion of tissue with collagenase and hyaluronidase resulted in cell clusters (organoids) resembling ductal and alveolar structures; these could be separated by filtration from the stromal components. Epithelial outgrowth from these organoids was greatly enhanced by the addition of conditioned medium from other human epithelial and myoepithelial cell lines. Additionally, the mammary epithelial growth was stimulated by insulin, hydrocortisone, epidermal growth factor, and steroid hormones. With this enriched nutritional environment, active cell division could be maintained for 1 to 3 months and cells could be serially subcultured 1 to 4 times.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Non-Profit & Charity
Science
Education

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,642,828 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure if the website is profiting.

Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Link.springer.com might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

human, google, scholar, pubmed, cas, mammary, cells, article, epithelial, cancer, cell, growth, breast, culture, normal, tissue, natl, vitro, inst, cultures, research, smith, springer, privacy, cookies, content, hallowes, hackett, lines, access, res, information, publish, search, stampfer, hormones, carcinomas, data, log, journal, medium, myoepithelial, tissues, chapter, discover, gaffney, stoker, int, pigott, nature,

Topics {✒️}

serum-free hormone-supplemented medium month download article/chapter myoepithelial cell lines conditioned medium obtain short-term cultures human mammary epithelial mammary epithelial growth aneuploid mammary epithelial neoplastic mammary epithelium human tumor cells cell tissue res human breast tissue epithelial cell cultures syngeneic cell lines privacy choices/manage cookies active cell division full article pdf abnormal breast tissue human breast carcinomas human breast tissues malignant breast tumors cancerous human tissues human lacteal secretions epithelial cells european economic area scope submit manuscript epidermal growth factor enriched nutritional environment related subjects epidermal growth factors embryonic mesenchyme resulting human cells conditions privacy policy electron microscope study tissue culture check access instant access cell lines american cancer society human epithelial accepting optional cookies diploid myoepithelial surface differentiation antigens cells derived tumor cells taylor-papadimitriou main content log buehring journal finder publish cell clusters

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Growth of normal human mammary cells in culture
         description:Reduction mammoplasty tissue was used to obtain short-term cultures of human epithelial cell populations. Digestion of tissue with collagenase and hyaluronidase resulted in cell clusters (organoids) resembling ductal and alveolar structures; these could be separated by filtration from the stromal components. Epithelial outgrowth from these organoids was greatly enhanced by the addition of conditioned medium from other human epithelial and myoepithelial cell lines. Additionally, the mammary epithelial growth was stimulated by insulin, hydrocortisone, epidermal growth factor, and steroid hormones. With this enriched nutritional environment, active cell division could be maintained for 1 to 3 months and cells could be serially subcultured 1 to 4 times.
         datePublished:
         dateModified:
         pageStart:415
         pageEnd:425
         sameAs:https://doi.org/10.1007/BF02618365
         keywords:
            human mammary epithelial cells
            myoepithelial cells
            conditioned medium
            hormones
            Plant Sciences
            Cell Biology
            Developmental Biology
            Plant Breeding/Biotechnology
            Plant Genetics and Genomics
         image:
         isPartOf:
            name:In Vitro
            issn:
               1475-2689
               0073-5655
            volumeNumber:16
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:M. Stampfer
               affiliation:
                     name:Peralta Cancer Research Institute
                     address:
                        name:Peralta Cancer Research Institute, Oakland
                        type:PostalAddress
                     type:Organization
                     name:Imperial Cancer Research Fund
                     address:
                        name:Imperial Cancer Research Fund, London, England
                        type:PostalAddress
                     type:Organization
               type:Person
               name:R. C. Hallowes
               affiliation:
                     name:Imperial Cancer Research Fund
                     address:
                        name:Imperial Cancer Research Fund, London, England
                        type:PostalAddress
                     type:Organization
               type:Person
               name:A. J. Hackett
               affiliation:
                     name:Peralta Cancer Research Institute
                     address:
                        name:Peralta Cancer Research Institute, Oakland
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Growth of normal human mammary cells in culture
      description:Reduction mammoplasty tissue was used to obtain short-term cultures of human epithelial cell populations. Digestion of tissue with collagenase and hyaluronidase resulted in cell clusters (organoids) resembling ductal and alveolar structures; these could be separated by filtration from the stromal components. Epithelial outgrowth from these organoids was greatly enhanced by the addition of conditioned medium from other human epithelial and myoepithelial cell lines. Additionally, the mammary epithelial growth was stimulated by insulin, hydrocortisone, epidermal growth factor, and steroid hormones. With this enriched nutritional environment, active cell division could be maintained for 1 to 3 months and cells could be serially subcultured 1 to 4 times.
      datePublished:
      dateModified:
      pageStart:415
      pageEnd:425
      sameAs:https://doi.org/10.1007/BF02618365
      keywords:
         human mammary epithelial cells
         myoepithelial cells
         conditioned medium
         hormones
         Plant Sciences
         Cell Biology
         Developmental Biology
         Plant Breeding/Biotechnology
         Plant Genetics and Genomics
      image:
      isPartOf:
         name:In Vitro
         issn:
            1475-2689
            0073-5655
         volumeNumber:16
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:M. Stampfer
            affiliation:
                  name:Peralta Cancer Research Institute
                  address:
                     name:Peralta Cancer Research Institute, Oakland
                     type:PostalAddress
                  type:Organization
                  name:Imperial Cancer Research Fund
                  address:
                     name:Imperial Cancer Research Fund, London, England
                     type:PostalAddress
                  type:Organization
            type:Person
            name:R. C. Hallowes
            affiliation:
                  name:Imperial Cancer Research Fund
                  address:
                     name:Imperial Cancer Research Fund, London, England
                     type:PostalAddress
                  type:Organization
            type:Person
            name:A. J. Hackett
            affiliation:
                  name:Peralta Cancer Research Institute
                  address:
                     name:Peralta Cancer Research Institute, Oakland
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:In Vitro
      issn:
         1475-2689
         0073-5655
      volumeNumber:16
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Peralta Cancer Research Institute
      address:
         name:Peralta Cancer Research Institute, Oakland
         type:PostalAddress
      name:Imperial Cancer Research Fund
      address:
         name:Imperial Cancer Research Fund, London, England
         type:PostalAddress
      name:Imperial Cancer Research Fund
      address:
         name:Imperial Cancer Research Fund, London, England
         type:PostalAddress
      name:Peralta Cancer Research Institute
      address:
         name:Peralta Cancer Research Institute, Oakland
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:M. Stampfer
      affiliation:
            name:Peralta Cancer Research Institute
            address:
               name:Peralta Cancer Research Institute, Oakland
               type:PostalAddress
            type:Organization
            name:Imperial Cancer Research Fund
            address:
               name:Imperial Cancer Research Fund, London, England
               type:PostalAddress
            type:Organization
      name:R. C. Hallowes
      affiliation:
            name:Imperial Cancer Research Fund
            address:
               name:Imperial Cancer Research Fund, London, England
               type:PostalAddress
            type:Organization
      name:A. J. Hackett
      affiliation:
            name:Peralta Cancer Research Institute
            address:
               name:Peralta Cancer Research Institute, Oakland
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Peralta Cancer Research Institute, Oakland
      name:Imperial Cancer Research Fund, London, England
      name:Imperial Cancer Research Fund, London, England
      name:Peralta Cancer Research Institute, Oakland
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(87)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.25s.

LINK . SPRINGER . COM {}