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Title:
Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma | In Vitro Cellular & Developmental Biology - Plant
Description:
An epithelial cell line, MDA-MB-157, has been established from the pleural fluid of a metastatic human breast carcinoma. The cell line is characterized by rapid growth of cells in dense plaques and in suspension. The cells were originally isolated in a complex medium containing Leibovitz
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cell, article, human, breast, cancer, google, scholar, cells, carcinoma, pubmed, cas, epithelial, line, access, natl, inst, texas, privacy, cookies, content, pleural, information, publish, search, vitro, establishment, young, cailleau, mackay, tumor, media, data, log, journal, research, mdamb, metastatic, effusion, january, dsc, reeves, growth, fetal, calf, serum, open, products, discover, cultivation, download,
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epithelial cell line month download article/chapter human breast carcinoma human mammary gland contract nih-nci-g-72-3869 pleural fluid cell line human breast cancer cell dispersion agent human breast carcinomas adenocarcinoma cell strains infiltrating duct carcinoma epithelial tumors metastatic pleural effusion pleural effusion derived tumor cells privacy choices/manage cookies related subjects full article pdf cell strains check access instant access cell biol rapid growth european economic area scope submit manuscript discontinuous density gradients national cancer institute conditions privacy policy january 1974 volume 9 growth dynamics malignant lung tissue rapid banding technique tumor institute accepting optional cookies mda-mb-157 texas medical center main content log cells exhibit journal finder publish article log human origin article cite human chromosomes privacy policy personal data article young cancer inst cancer res cells
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headline:Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma
description:An epithelial cell line, MDA-MB-157, has been established from the pleural fluid of a metastatic human breast carcinoma. The cell line is characterized by rapid growth of cells in dense plaques and in suspension. The cells were originally isolated in a complex medium containing Leibovitz's L-15, Waymouth's MB752/1 and Ham's F-10 media with 16% fetal calf serum, glutathione, cortisol, insulin, and antibiotics. Recently cultures have been growing equally well on some less complex media such as RPMI-1640 with 20% fetal calf serum or Ham's F-10 with 20% fetal calf serum. The presence of typical epithelial cell organization, and structures such as desmosomes, have been observed by electron microscopy. Viruses and mycoplasmas were not detected. The cells exhibit a hypotriploid karyotype with mode of 64 to 66 chromosomes.
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Epithelial Cell Line
Pleural Fluid
Human Breast Carcinoma
Viable Tumor Cell
Breast Carcinoma Cell Line
Plant Sciences
Cell Biology
Developmental Biology
Plant Breeding/Biotechnology
Plant Genetics and Genomics
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headline:Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma
description:An epithelial cell line, MDA-MB-157, has been established from the pleural fluid of a metastatic human breast carcinoma. The cell line is characterized by rapid growth of cells in dense plaques and in suspension. The cells were originally isolated in a complex medium containing Leibovitz's L-15, Waymouth's MB752/1 and Ham's F-10 media with 16% fetal calf serum, glutathione, cortisol, insulin, and antibiotics. Recently cultures have been growing equally well on some less complex media such as RPMI-1640 with 20% fetal calf serum or Ham's F-10 with 20% fetal calf serum. The presence of typical epithelial cell organization, and structures such as desmosomes, have been observed by electron microscopy. Viruses and mycoplasmas were not detected. The cells exhibit a hypotriploid karyotype with mode of 64 to 66 chromosomes.
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Pleural Fluid
Human Breast Carcinoma
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Breast Carcinoma Cell Line
Plant Sciences
Cell Biology
Developmental Biology
Plant Breeding/Biotechnology
Plant Genetics and Genomics
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