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Title:
Identifikation von Merozoiten der vier cystenbildenden Coccidien (Sarcocystis, Toxoplasma, Besnoitia, Frenkelia) auf Grund feinstruktureller Kriterien | Parasitology Research
Description:
Cyst stages of the speciesSarcocystis tenella, Frenkelia spec.,Besnoitia jellisoni, andToxoplasma gondii are compared on the electron microscope. Ultrastructural differences between these 4 species are found sufficient to serve as a basis for their exact taxonomic identification. Merozoites, which closely resemble merozoites of other coccidia in regard to shape and ultrastructure, are found in cysts of all 4 species. In addition to them, cysts ofS. tenella andFrenkelia spec. contain metrocytes which are located at the periphery. Metrocytes are ovoid and have deep infoldings that give them the appearance of separated cells. The typical organelles of merozoites such as conoids and micropores are always found in metrocytes. Usually, more than 2 micropores are present. In both species, the nucleus shows a spherical area of osmiophilic granules in addition to the nucleoli. Besides metrocytes,Frenkelia spec. has so-called intermediate cells of bipolar organization that have micronemes at both ends. InB. jellisoni andT. gondii only merozoites were found inside the cysts. Those ofB. jellisoni differ distinctly from merozoites of any other species in having membrane-bound, electronlight spindle-shaped bodies with an electrondense core (=enigmatic bodies). Their function is not yet understood. Exclusive criteria for the different species are:S. tenella has metrocytes and merozoites which are twice as large as those of any other genus. Micronemes in merozoites are abundant (about 400), and up to 11 rhoptries can be found.Frenkelia spec. has intermediate cells with bipolar organization and merozoites with a distinct posterior cone. Micronemes in merozoites are limited to 50â70, and 5â8 rhoptries are present.B. jellisoni merozoites have 80â100 micronemes, 3â5 rhoptries, and 20â30 enigmatic bodies.T. gondii merozoites are the smallest of all species examined, and the number of micronemes is rather small, too, and does not exceed 50. All stages of the 4 species multiply by endodyogeny. Except of metrocytes and intermediate cells ofFrenkelia spec., 22 subpellicular microtubules are found in all stages. The meanings of these findings in regard to their species- or genus-specific significance and several implications on the functional morphology of some organelles are given in the discussion.
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Keywords {đ}
google, scholar, article, cas, merozoites, gondii, pubmed, scholtyseck, jellisoni, spec, tenella, species, merozoiten, mehlhorn, frenkelia, electron, micronemes, protistologica, parasitenk, privacy, cookies, content, found, metrocytes, cells, organelles, mikronemen, access, information, publish, search, coccidien, kriterien, cyst, cysts, metrocyten, arten, rhoptrien, related, parasit, structure, untersuchungen, data, log, journal, research, parasitenkunde, identifikation, sarcocystis, toxoplasma,
Topics {âïž}
related subjects month download article/chapter und dem sog merozoiten von eimerien hervorgegangen durch endodyogenie die mikronemen treten die sporozyste vons full article pdf electronlight spindle-shaped bodies die nahezu doppelt desgleichen die rhoptrien 1007/bf02482636 access lakunen des er cystenstadien vonbesnoitia jellisoni article scholtyseck privacy choices/manage cookies related organisms ĂŒber eigenthĂŒmliche schlĂ€uche ou organismes voisins check access article log instant access electron microscope study article cite exact taxonomic identification subpellikulĂ€re mikrotubuli tenella andfrenkelia spec tenella undfrenkelia spec jellisoni differ distinctly cyst oftoxoplasma gondii reproduction oftoxoplasma gondii distinct posterior cone enigmatische körper charakterisiert atti vi congr ciliĂ© holotriche gymnostome ciliated protozoondidinium nasutum identity oftoxoplasma nicolle undtoxoplasma gondii donnĂ©es ultrastructurales complĂ©mentaires conditions privacy policy called intermediate cells european economic area der cystengrundsubstanz der conoidspitze van der des toxoplasmes 4 cyst-forming coccidia accepting optional cookies genus-specific significance heinz mehlhorn & bodo
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- Osmiophile Körperchen im Karyoplasma (Spiralstruktur?
- Polysaccharidgrana (Amylopektin?
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headline:Identifikation von Merozoiten der vier cystenbildenden Coccidien (Sarcocystis, Toxoplasma, Besnoitia, Frenkelia) auf Grund feinstruktureller Kriterien
description:Die Cystenstadien vonSarcocystis tenella, Frenkelia spec.,Besnoitia jellisoni undToxoplasma gondii wurden elektronenoptisch untersucht und schematisch in Relation zu ihrer GröĂe dargestellt. Mikromorphologische Unterschiede erlauben eine eindeutige Identifikation dieser Coccidien. In allen Cysten sind Merozoiten enthalten, stets von bananenförmiger Gestalt. DarĂŒber hinaus weisen die Cysten vonS. tenella undFrenkelia spec. noch ovoide Metrocyten mit tiefen Einfaltungen auf. Sie liegen an der Peripherie der Cyste, dicht unterhalb der begrenzenden PrimĂ€rhĂŒlle. Die Metrocyten beider Arten zeigen typische Merozoitenorganelle und sind auĂerdem noch durch einen walzenförmigen bzw. kugeligen Bereich osmiophiler Grana im Nukleus ausgezeichnet. BeiFrenkelia spec. wird noch ein dritter Zelltypus ausgebildet, der als âintermediĂ€re Zelleâ bezeichnet wird und bipolar aufgebaut ist. Als wichtige Kriterien der untersuchten Arten wurden folgende Merkmale herangezogen:S. tenella besitzt Metrocyten und Merozoiten, die nahezu doppelt so groĂ sind wie die der ĂŒbrigen Arten. Die Mikronemen treten in auĂergewöhnlich groĂer Anzahl auf (ca. 400), desgleichen die Rhoptrien (ca. 11).Frenkelia spec. bildet bipolare intermediĂ€re Zellen aus. Die Merozoiten zeigen einen besonderen hinteren Conus. Die Anzahl der Mikronemen ist auf etwa 50â70 beschrĂ€nkt; etwa 5â8 Rhoptrien sind vorhanden.B. jellisoni-Merozoiten sind durch 20â30 sog. enigmatische Körper charakterisiert; etwa 80â100 Mikronemen und ca. 3â5 Rhoptrien sind vorhanden.T. gondii weist die kleinsten Merozoiten aller untersuchten Arten auf. Die Anzahl der Mikronemen ĂŒbersteigt 50 kaum. Alle hier untersuchten Stadien vermehren sich durch Endodyogenie. Mit Ausnahme der Metrocyten und intermediĂ€ren Zellen vonFrenkelia spec. konnten bei allen Formen 22 subpellikulĂ€re Mikrotubuli nachgewiesen werden. Die feinstrukturellen Ergebnisse wurden abschlieĂend auf ihren Wert als systematische Kriterien geprĂŒft.
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Immunology
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headline:Identifikation von Merozoiten der vier cystenbildenden Coccidien (Sarcocystis, Toxoplasma, Besnoitia, Frenkelia) auf Grund feinstruktureller Kriterien
description:Die Cystenstadien vonSarcocystis tenella, Frenkelia spec.,Besnoitia jellisoni undToxoplasma gondii wurden elektronenoptisch untersucht und schematisch in Relation zu ihrer GröĂe dargestellt. Mikromorphologische Unterschiede erlauben eine eindeutige Identifikation dieser Coccidien. In allen Cysten sind Merozoiten enthalten, stets von bananenförmiger Gestalt. DarĂŒber hinaus weisen die Cysten vonS. tenella undFrenkelia spec. noch ovoide Metrocyten mit tiefen Einfaltungen auf. Sie liegen an der Peripherie der Cyste, dicht unterhalb der begrenzenden PrimĂ€rhĂŒlle. Die Metrocyten beider Arten zeigen typische Merozoitenorganelle und sind auĂerdem noch durch einen walzenförmigen bzw. kugeligen Bereich osmiophiler Grana im Nukleus ausgezeichnet. BeiFrenkelia spec. wird noch ein dritter Zelltypus ausgebildet, der als âintermediĂ€re Zelleâ bezeichnet wird und bipolar aufgebaut ist. Als wichtige Kriterien der untersuchten Arten wurden folgende Merkmale herangezogen:S. tenella besitzt Metrocyten und Merozoiten, die nahezu doppelt so groĂ sind wie die der ĂŒbrigen Arten. Die Mikronemen treten in auĂergewöhnlich groĂer Anzahl auf (ca. 400), desgleichen die Rhoptrien (ca. 11).Frenkelia spec. bildet bipolare intermediĂ€re Zellen aus. Die Merozoiten zeigen einen besonderen hinteren Conus. Die Anzahl der Mikronemen ist auf etwa 50â70 beschrĂ€nkt; etwa 5â8 Rhoptrien sind vorhanden.B. jellisoni-Merozoiten sind durch 20â30 sog. enigmatische Körper charakterisiert; etwa 80â100 Mikronemen und ca. 3â5 Rhoptrien sind vorhanden.T. gondii weist die kleinsten Merozoiten aller untersuchten Arten auf. Die Anzahl der Mikronemen ĂŒbersteigt 50 kaum. Alle hier untersuchten Stadien vermehren sich durch Endodyogenie. Mit Ausnahme der Metrocyten und intermediĂ€ren Zellen vonFrenkelia spec. konnten bei allen Formen 22 subpellikulĂ€re Mikrotubuli nachgewiesen werden. Die feinstrukturellen Ergebnisse wurden abschlieĂend auf ihren Wert als systematische Kriterien geprĂŒft.
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Medical Microbiology
Microbiology
Immunology
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