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Title:
Oncogene-induced basement membrane invasiveness in human mammary epithelial cells | Clinical & Experimental Metastasis
Description:
Expression of the intermediate filament protein vimentin, and loss of the cellular adhesion protein uvomorulin (E-cadherin) have been associated with increased invasiveness of established human breast cancer cell linesin vitro andin vivo. In the current study, we have further examined these relationships in oncogenically transformed human mammary epithelial cells. A normal human mammary epithelial strain, termed 184, was previously immortalized with benzo[a]pyrene, and two distinct sublines were derived (A1N4 and 184B5). These sublines were infected with retroviral vectors containing a single or two oncogenes of the nuclear, cytoplasmic, and plasma membrane-associated type (v-ras H, v-ras Ki, v -mos, SV40T and c -myc). All infectants have been previously shown to exhibit some aspects of phenotypic transformation. In the current study, cellular invasiveness was determinedin vitro using Matrigel, a reconstituted basement membrane extract. Lineage-specific differences were observed with respect to low constitutive invasiveness and invasive changes after infection withras, despite similarras-induced transformation of each line. Major effects on cellular invasiveness were observed after infection of the cells with two different oncogenes (v-ras H + SV40T and v -ras H + v -mos). In contrast, the effects of single oncogenes were only modest or negligible. All oncogenic infectants demonstrated increased attachment to laminin, but altered secretion of the 72 kDa and 92 kDa gelatinases was not associated with any aspect of malignant progression. Each of the two highly invasive double oncogene transformants were vimentinpositive and uvomorulin-negative, a phenotype indicative of the epithelial-mesenchymal transition (EMT) previously associated with invasiveness of established human breast cancer cell lines. Weakly invasive untransformed mammary epithelial cells in this study were positive for both vimentin and uvomorulin, suggesting that uvomorulin may over-ride the otherwise vimentin-associated invasiveness.
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Keywords {π}
google, scholar, human, cancer, cell, breast, cells, epithelial, mammary, res, metastasis, type, invasive, article, expression, basement, thompson, liotta, collagenase, membrane, invasiveness, vimentin, invasion, tumor, usa, normal, growth, metastatic, stampfer, vitro, pathol, research, sommers, dickson, cellular, adhesion, oncogenes, laminin, malignant, natl, privacy, cookies, content, torri, lippman, uvomorulin, increased, transformation, potential, receptor,
Topics {βοΈ}
c-erbb2/neu oncogeneinduced metastasis critical etv4/twist1/vimentin axis epithelial-mesenchymal transition month download article/chapter increased basement membrane-invasiveness node-negative breast cancers nih/3t3 cells transfected mcf-7 cells transfected matrigel-basedin vitro assays basement membrane invasion v-ras ki human breast carcinomas basement membrane invasiveness rat mammary cells cancer-derived extracellular vesicles basement membrane complexes cell surface properties luminal epithelial cells extracellular matrix-degrading potential human carcinoma cells human mammary gland neoplastic breast epithelium normal human macrophages gerontology research center similarras-induced transformation full article pdf lobular breast carcinomas privacy choices/manage cookies cell adhesion molecules human tumor dna related subjects v-ras hormone-responsive variants stetler-stevensen wg cell growth differ breast carcinoma progression rapidin vitro assay ki67 proliferation antigen natl cancer inst tumor cell migration cancer metastasis rev specific adult tissues malignant breast disease molec cell endocr basement membrane stetler-stevenson wg malignant breast tissue expl cell res low constitutive invasiveness clin expl metastasis
Questions {β}
- Raymond WA and Leong AS-Y, 1989, Vimentin-a new prognostic parameter in breast carcinoma?
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headline:Oncogene-induced basement membrane invasiveness in human mammary epithelial cells
description:Expression of the intermediate filament protein vimentin, and loss of the cellular adhesion protein uvomorulin (E-cadherin) have been associated with increased invasiveness of established human breast cancer cell linesin vitro andin vivo. In the current study, we have further examined these relationships in oncogenically transformed human mammary epithelial cells. A normal human mammary epithelial strain, termed 184, was previously immortalized with benzo[a]pyrene, and two distinct sublines were derived (A1N4 and 184B5). These sublines were infected with retroviral vectors containing a single or two oncogenes of the nuclear, cytoplasmic, and plasma membrane-associated type (v-ras
H, v-ras
Ki, v -mos, SV40T and c -myc). All infectants have been previously shown to exhibit some aspects of phenotypic transformation. In the current study, cellular invasiveness was determinedin vitro using Matrigel, a reconstituted basement membrane extract. Lineage-specific differences were observed with respect to low constitutive invasiveness and invasive changes after infection withras, despite similarras-induced transformation of each line. Major effects on cellular invasiveness were observed after infection of the cells with two different oncogenes (v-ras
H + SV40T and v -ras
H + v -mos). In contrast, the effects of single oncogenes were only modest or negligible. All oncogenic infectants demonstrated increased attachment to laminin, but altered secretion of the 72 kDa and 92 kDa gelatinases was not associated with any aspect of malignant progression. Each of the two highly invasive double oncogene transformants were vimentinpositive and uvomorulin-negative, a phenotype indicative of the epithelial-mesenchymal transition (EMT) previously associated with invasiveness of established human breast cancer cell lines. Weakly invasive untransformed mammary epithelial cells in this study were positive for both vimentin and uvomorulin, suggesting that uvomorulin may over-ride the otherwise vimentin-associated invasiveness.
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basement membrane
human breast cancer
invasion
oncogene
uvomorulin
vimentin
Cancer Research
Biomedicine
general
Oncology
Hematology
Surgical Oncology
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headline:Oncogene-induced basement membrane invasiveness in human mammary epithelial cells
description:Expression of the intermediate filament protein vimentin, and loss of the cellular adhesion protein uvomorulin (E-cadherin) have been associated with increased invasiveness of established human breast cancer cell linesin vitro andin vivo. In the current study, we have further examined these relationships in oncogenically transformed human mammary epithelial cells. A normal human mammary epithelial strain, termed 184, was previously immortalized with benzo[a]pyrene, and two distinct sublines were derived (A1N4 and 184B5). These sublines were infected with retroviral vectors containing a single or two oncogenes of the nuclear, cytoplasmic, and plasma membrane-associated type (v-ras
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Ki, v -mos, SV40T and c -myc). All infectants have been previously shown to exhibit some aspects of phenotypic transformation. In the current study, cellular invasiveness was determinedin vitro using Matrigel, a reconstituted basement membrane extract. Lineage-specific differences were observed with respect to low constitutive invasiveness and invasive changes after infection withras, despite similarras-induced transformation of each line. Major effects on cellular invasiveness were observed after infection of the cells with two different oncogenes (v-ras
H + SV40T and v -ras
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basement membrane
human breast cancer
invasion
oncogene
uvomorulin
vimentin
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Oncology
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Surgical Oncology
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- How much revenue does http://scholar.google.com/scholar_lookup?&title=Immunohistochemical%20distribution%20of%20type%20IV%20collagenase%20in%20normal%2C%20benign%2C%20and%20malignant%20breast%20tissue&journal=Am%20J%20Pathol&volume=136&pages=585-92&publication_year=1990&author=Monteagudo%2CC&author=Merino%2CMJ&author=San-Juan%2CJ&author=Liotta%2CLA&author=Stetler-Stevensen%2CWG generate?
- Income figures for http://scholar.google.com/scholar_lookup?&title=Laminin%20receptors%2C%20collagenase%20IV%20and%20prognosis%20in%20node-negative%20breast%20cancers&journal=Int%20J%20Cancer&volume=48&pages=529-32&publication_year=1991&author=Daidone%2CMG&author=Silvestrini%2CR&author=D%27Errico%2CA
- Income figures for http://scholar.google.com/scholar_lookup?&title=Augmentation%20of%20type%20IV%20collagenase%2C%20laminin%20receptor%2C%20and%20Ki67%20proliferation%20antigen%20associated%20with%20human%20colon%2C%20gastric%2C%20and%20breast%20carcinoma%20progression&journal=Modern%20Pathol&volume=4&pages=239-46&publication_year=1991&author=D%27Errico%2CA&author=Garbisa%2CS&author=Liotta%2CLA
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