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Title:
Visualization of chromosomes in mitotically arrested cells of the fission yeast Schizosaccharomyces pombe | Current Genetics
Description:
Three sets of mitotic chromosomes were observed in wild type or cdc mutants (nda3-KM311 and nda2-KM52) of the fission yeast S. pombe by the DAPI staining method. The block of microtubular functions by thiabendazole or by the mutations caused their individual appearance in mitotically arrested cells. The chromosomes have a characteristic size; the length ratio of short, medium and long ones was roughly 1:2:3, consistent with the previous genetical data (Kohli et al. 1977). Double staining with ethidium bromide and DAPI showed that the nucleolus was always associated with the shortest chromosome. Pair-like structures resembling sister chromatids were also seen.
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google, scholar, article, cell, genetics, chromosomes, toda, yanagida, access, privacy, cookies, content, umesono, mitotic, biol, data, publish, search, fission, yeast, schizosaccharomyces, pombe, chromosome, sci, mol, information, log, journal, research, current, mitotically, arrested, cells, hiraoka, dapi, open, discover, leupold, download, springer, optional, personal, parties, policy, find, track, visualization, cite, explore, cdc,
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month download article/chapter mitotic chromatin landscape 6-diamidino-2-phenylindole kb privacy choices/manage cookies full article pdf related subjects mitotic chromosomes fission yeast shortest chromosome european economic area mitotically arrested cells scope submit manuscript cdc mutants previous genetical data conditions privacy policy dapi staining method accepting optional cookies current genetics 7 journal finder publish check access instant access article log robinow df article cite yanagida rights personal data privacy policy article umesono pombe schizosaccharomyces books a cell biol 72 cell sci 24 cell sci 9 cell sci 52 optional cookies mol biol 152 mol biol manage preferences dapi showed subscription content similar content data protection essential cookies cookies skip double staining visualization institution subscribe cell biology journal publish
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headline:Visualization of chromosomes in mitotically arrested cells of the fission yeast Schizosaccharomyces pombe
description:Three sets of mitotic chromosomes were observed in wild type or cdc mutants (nda3-KM311 and nda2-KM52) of the fission yeast S. pombe by the DAPI staining method. The block of microtubular functions by thiabendazole or by the mutations caused their individual appearance in mitotically arrested cells. The chromosomes have a characteristic size; the length ratio of short, medium and long ones was roughly 1:2:3, consistent with the previous genetical data (Kohli et al. 1977). Double staining with ethidium bromide and DAPI showed that the nucleolus was always associated with the shortest chromosome. Pair-like structures resembling sister chromatids were also seen.
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Chromosome
4,6-diamidino-2-phenylindole
Mitosis
Fluorescence microscopy
Microbial Genetics and Genomics
Microbiology
Biochemistry
general
Cell Biology
Plant Sciences
Proteomics
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headline:Visualization of chromosomes in mitotically arrested cells of the fission yeast Schizosaccharomyces pombe
description:Three sets of mitotic chromosomes were observed in wild type or cdc mutants (nda3-KM311 and nda2-KM52) of the fission yeast S. pombe by the DAPI staining method. The block of microtubular functions by thiabendazole or by the mutations caused their individual appearance in mitotically arrested cells. The chromosomes have a characteristic size; the length ratio of short, medium and long ones was roughly 1:2:3, consistent with the previous genetical data (Kohli et al. 1977). Double staining with ethidium bromide and DAPI showed that the nucleolus was always associated with the shortest chromosome. Pair-like structures resembling sister chromatids were also seen.
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4,6-diamidino-2-phenylindole
Mitosis
Fluorescence microscopy
Microbial Genetics and Genomics
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Cell Biology
Plant Sciences
Proteomics
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