We are analyzing https://link.springer.com/article/10.1007/bf00323577.

Title:
A morphological and immunohistochemical study of programmed cell death in Botryllus schlosseri (Tunicata, Ascidiacea) | Cell and Tissue Research
Description:
The blastogenic cycle of the colonial ascidian Botryllus schlosseri concludes in a phase of selective cell and zooid death called takeover. Every week, all asexually derived parental zooids synchronously regress over a 30-h period and are replaced by a new generation. Here we document the sequential ultrastructural changes which accompany cell death during zooid degeneration. The principal mode of visceral cell death during takeover occurred by apoptosis, the majority of cells condensing and fragmenting into multiple membrane-bounded apoptotic bodies. Cytoplasmic organelles (mitochondria, basal bodies, striated rootlets) within apoptotic bodies retained ultrastructural integrity. Dying cells and fragments were then swiftly ingested by specialized blood macrophages or intraepithelial phagocytes and subsequently underwent secondary necrotic lysis. Certain organs (stomach, intestine) displayed a combination of necrotic and apoptotic changes. Lastly, the stomach, which demonstrated some of the earliest regressive changes, exhibited intense cytoplasmic immunostaining with a monoclonal antibody to ubiquitin at the onset of takeover. Affinity-purified rabbit antiserum against sodium dodecyl sulfate-denatured ubiquitin detected a characteristic 8.6-kDa mono-ubiquitin band by Western blot analysis. Collectively, these findings raise the possibility that cell death during takeover is a dynamic process which requires active participation of cells in their own destruction.
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Keywords {🔍}

google, scholar, cell, death, apoptosis, programmed, article, cells, botryllus, usa, weissman, sci, nature, schlosseri, wyllie, research, tissue, lauzon, ubiquitin, access, biol, exp, privacy, cookies, content, study, ultrastructural, protein, immunol, publish, search, colonial, takeover, metamorphosis, morphol, science, dna, fragmentation, proc, natl, acad, embryol, rev, target, albany, medical, data, information, log, journal,

Topics {✒️}

month download article/chapter 6-kda mono-ubiquitin band vitronectin receptor-mediated phagocytosis radiation-induced interphase death affinity-purified rabbit antiserum hormone-induced cell death intracellular ubiquitin-protein conjugates targeted short-lived protein ubiquitin-mediated protein degradation cd4+cd8+tcrlo thymocytes activation-induced cell death glucocorticoid-induced thymocyte apoptosis basic biological phenomenon cytolytic lymphocytes induce specialized blood macrophages botryllus schlosseri pallas privacy choices/manage cookies programmed cell death beckman research center full article pdf accompany cell death visceral cell death cell-mediated cytolysis tissue research aims endogenous suicide process endogenous endonuclease activation european economic area requires active participation related subjects posterior necrotic zone wide-ranging implications ventral nerve cord thymocytes undergoing apoptosis conditions privacy policy western blot analysis mitophagy response leads chick wing-bud striated muscle fibers walport mj polyubiquitin gene expression albany medical college caenorhabditis elegans larvae botryllus schlosseri electron-microscope study accepting optional cookies autoreactive blood cells anuran tadpole tail cells undergoing apoptosis cell sci 14 main content log

Questions {❓}

A cell’s agony of choice: how to cross the Styx?
Weissman IL, Saito Y, Rinkevich B (1990) Allorecognition histocompatibility in a protochordate species: is the relationship to MHC semantic or structural?

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      description:The blastogenic cycle of the colonial ascidian Botryllus schlosseri concludes in a phase of selective cell and zooid death called takeover. Every week, all asexually derived parental zooids synchronously regress over a 30-h period and are replaced by a new generation. Here we document the sequential ultrastructural changes which accompany cell death during zooid degeneration. The principal mode of visceral cell death during takeover occurred by apoptosis, the majority of cells condensing and fragmenting into multiple membrane-bounded apoptotic bodies. Cytoplasmic organelles (mitochondria, basal bodies, striated rootlets) within apoptotic bodies retained ultrastructural integrity. Dying cells and fragments were then swiftly ingested by specialized blood macrophages or intraepithelial phagocytes and subsequently underwent secondary necrotic lysis. Certain organs (stomach, intestine) displayed a combination of necrotic and apoptotic changes. Lastly, the stomach, which demonstrated some of the earliest regressive changes, exhibited intense cytoplasmic immunostaining with a monoclonal antibody to ubiquitin at the onset of takeover. Affinity-purified rabbit antiserum against sodium dodecyl sulfate-denatured ubiquitin detected a characteristic 8.6-kDa mono-ubiquitin band by Western blot analysis. Collectively, these findings raise the possibility that cell death during takeover is a dynamic process which requires active participation of cells in their own destruction.
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