We are analyzing https://link.springer.com/article/10.1007/bf00318659.

Title:
One-step transformation of yeast in stationary phase | Current Genetics
Description:
A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,642,828 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure if the website is profiting.

Some websites aren't about earning revenue; they're built to connect communities or raise awareness. There are numerous motivations behind creating websites. This might be one of them. Link.springer.com has a secret sauce for making money, but we can't detect it yet.

Keywords {🔍}

google, scholar, article, privacy, cookies, transformation, kuo, content, information, publish, search, genetics, yeast, stationary, phase, chen, tsongteh, access, data, log, journal, research, dzchi, yang, cells, discover, ito, murata, kimura, agric, biol, chem, download, institute, taipei, republic, china, springer, optional, personal, parties, policy, find, track, current, onestep, cite, beichang, explore, thio,

Topics {✒️}

fast yeast-transformation technique month download article/chapter alkali-ion based protocols adding thio compounds privacy choices/manage cookies full article pdf stationary phase european economic area national taiwan university conditions privacy policy article chen accepting optional cookies main content log journal finder publish check access instant access step transformation hinnen rights kuo yang related subjects privacy policy personal data transformation books a article log yeast cells optional cookies manage preferences dna samples article cite subscription content data protection essential cookies cookies skip information institution subscribe journal publish usage analysis social media varying standards january 1992 volume 21 latest articles machine learning gen microbiol 132 hicks jb fink gr harriss jv sharp jd douglas mg

Schema {🗺️}

WebPage:
      mainEntity:
         headline:One-step transformation of yeast in stationary phase
         description:A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
         datePublished:
         dateModified:
         pageStart:83
         pageEnd:84
         sameAs:https://doi.org/10.1007/BF00318659
         keywords:
            Yeast
            Transformation
            Thio compound
            Stationary phase
            Microbial Genetics and Genomics
            Microbiology
            Biochemistry
            general
            Cell Biology
            Plant Sciences
            Proteomics
         image:
         isPartOf:
            name:Current Genetics
            issn:
               1432-0983
               0172-8083
            volumeNumber:21
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Dz-Chi Chen
               affiliation:
                     name:National Taiwan University
                     address:
                        name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
                        type:PostalAddress
                     type:Organization
                     name:Academia Sinica
                     address:
                        name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Bei-Chang Yang
               affiliation:
                     name:Academia Sinica
                     address:
                        name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Tsong-Teh Kuo
               affiliation:
                     name:National Taiwan University
                     address:
                        name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
                        type:PostalAddress
                     type:Organization
                     name:Academia Sinica
                     address:
                        name:Institute of Botany, Academia Sinica, Taipei, Republic of China
                        type:PostalAddress
                     type:Organization
                     name:Academia Sinica
                     address:
                        name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:One-step transformation of yeast in stationary phase
      description:A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
      datePublished:
      dateModified:
      pageStart:83
      pageEnd:84
      sameAs:https://doi.org/10.1007/BF00318659
      keywords:
         Yeast
         Transformation
         Thio compound
         Stationary phase
         Microbial Genetics and Genomics
         Microbiology
         Biochemistry
         general
         Cell Biology
         Plant Sciences
         Proteomics
      image:
      isPartOf:
         name:Current Genetics
         issn:
            1432-0983
            0172-8083
         volumeNumber:21
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Dz-Chi Chen
            affiliation:
                  name:National Taiwan University
                  address:
                     name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
                     type:PostalAddress
                  type:Organization
                  name:Academia Sinica
                  address:
                     name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Bei-Chang Yang
            affiliation:
                  name:Academia Sinica
                  address:
                     name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tsong-Teh Kuo
            affiliation:
                  name:National Taiwan University
                  address:
                     name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
                     type:PostalAddress
                  type:Organization
                  name:Academia Sinica
                  address:
                     name:Institute of Botany, Academia Sinica, Taipei, Republic of China
                     type:PostalAddress
                  type:Organization
                  name:Academia Sinica
                  address:
                     name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Current Genetics
      issn:
         1432-0983
         0172-8083
      volumeNumber:21
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:National Taiwan University
      address:
         name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
         type:PostalAddress
      name:Academia Sinica
      address:
         name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
         type:PostalAddress
      name:Academia Sinica
      address:
         name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
         type:PostalAddress
      name:National Taiwan University
      address:
         name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
         type:PostalAddress
      name:Academia Sinica
      address:
         name:Institute of Botany, Academia Sinica, Taipei, Republic of China
         type:PostalAddress
      name:Academia Sinica
      address:
         name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Dz-Chi Chen
      affiliation:
            name:National Taiwan University
            address:
               name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
               type:PostalAddress
            type:Organization
            name:Academia Sinica
            address:
               name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
               type:PostalAddress
            type:Organization
      name:Bei-Chang Yang
      affiliation:
            name:Academia Sinica
            address:
               name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
               type:PostalAddress
            type:Organization
      name:Tsong-Teh Kuo
      affiliation:
            name:National Taiwan University
            address:
               name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
               type:PostalAddress
            type:Organization
            name:Academia Sinica
            address:
               name:Institute of Botany, Academia Sinica, Taipei, Republic of China
               type:PostalAddress
            type:Organization
            name:Academia Sinica
            address:
               name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
      name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
      name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
      name:Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
      name:Institute of Botany, Academia Sinica, Taipei, Republic of China
      name:Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(44)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.33s.

LINK . SPRINGER . COM {}