We are analyzing https://link.springer.com/article/10.1007/bf00218323.

Title:
Primary sensory neurons of the rat showing calcitonin gene-related peptide immunoreactivity and their relation to substance P-, somatostatin-, galanin-, vasoactive intestinal polypeptide- and cholecystokinin-immunoreactive ganglion cells | Cell and Tissue Research
Description:
By use of the indirect immunofluorescence technique the distribution of calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI) has been analyzed in cervical and lumbar dorsal root ganglia of untreated and colchicine-treated rats. In addition, lumbar ganglia were examined 2 weeks after transection of the sciatic nerve. The occurrence of CGRP-positive cells in relation to ganglion cells containing substance P-, somatostatin-, galanin-, cholecystokinin (CCK)-, and vasoactive intestinal polypeptide (VIP)/peptide histidine isoleucin (PHI)-LI has been evaluated on consecutive sections as well as using elution-restaining and double-staining techniques. CGRP-LI was observed in many ganglion cells of all sizes ranging in diameter from 15 μm to 65 μm. Thus, this peptide occurs also in the large primary sensory neurons. In contrast to the sensory peptides described to date, CGRP-positive cells constituted up to 50% of all and 70% of the medium-sized neurons, thus being the most frequently occurring peptide in sensory neurons so far encountered. Subpulations of CGRP-positive neurons were shown to contain substance P-, somatostatin-, or galanin-LI and some CGRP-positive neurons contained both substance P- and galanin-LI. In fact, most substance P-, somatostatin- and galanin-positive cell bodies were CGRP-immunoreactive. The coexistence analysis further revealed that galanin and substance P often coexisted and that some cells contained both substance P- and somatostatin-LI, whereas no coexistence between galanin and somatostatin has as yet been seen. VIP/PHI-LI was only shown in a few cells in untreated or colchicine-treated rats. However, after transcetion of the sciatic nerve numerous VIP/PHI-positive cells were observed, some of which also contained CGRP-LI. The present results indicate that a CGRP-like peptide is present in a wide range of primary sensory neurons probably not related to specific sensory modalities. Often this peptide coexists with other biologically active peptides. Taken together these findings suggest that CGRP may have a generalized function.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Health & Fitness

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don't see any clear sign of profit-making.

Earning money isn't the goal of every website; some are designed to offer support or promote social causes. People have different reasons for creating websites. This might be one such reason. Link.springer.com might be earning cash quietly, but we haven't detected the monetization method.

Keywords {🔍}

google, scholar, rat, peptide, substance, neurons, calcitonin, generelated, sensory, dorsal, spinal, brain, cord, res, distribution, ganglia, neurosci, hökfelt, immunoreactivity, root, nerve, vasoactive, intestinal, neuroscience, somatostatin, polypeptide, lett, cell, article, primary, peptides, coexistence, polak, peripheral, nervous, system, ganglion, cells, capsaicin, bloom, immunohistochemical, research, cholecystokinin, central, lundberg, gibson, tissue, galanin, fischer, cat,

Topics {✒️}

calcitonin gene-related peptide calcitonin gene-related peptide month download article/chapter zinc iodide-osmium impregnation fluoride-resistant acid phosphatase buffered picric-acid formaldehyde cgrp-positive cells constituted galanin-positive cell bodies cgrp-positive neurons contained cholecystokinin-immunoreactive ganglion cells privacy choices/manage cookies tissue research aims full article pdf fluorescent antibody methods dorsal root ganglia vasoactive intestinal peptide bovine dorsal root wander research institute related subjects primary afferent neuropeptides primary sensory neurons /peptide histidine isoleucin frequently occurring peptide powerful inhibitory peptide fluorescent protein tracing corticotropin releasing factor motoneuron-depolarizing peptide cgrp-positive cells primary nociceptive neuron vasoactive intestinal polypeptide vasoactive intestinal polypeptide cgrp-positive neurons neuronal cell bodies specific sensory modalities cell tissue res calcitonin gene visceral sensory neurons alternative rna-processing central nervous system sciatic nerve resection sciatic nerve section peripheral nervous system dorsal spinal cord lumbar dorsal horn scope submit manuscript double-staining techniques trigeminovascular nocisensor complex primary afferent fibers amine storage granules ciba foundation symposium

Questions {❓}

Ju G, Hökfelt T, Fischer JA, Frey P, Rehfeld JF, Dockray GJ (1986) Does cholecystokinin-like immunoreactivity in primary sensory neurons represent calcitonin gene-related peptide?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Primary sensory neurons of the rat showing calcitonin gene-related peptide immunoreactivity and their relation to substance P-, somatostatin-, galanin-, vasoactive intestinal polypeptide- and cholecystokinin-immunoreactive ganglion cells
         description:By use of the indirect immunofluorescence technique the distribution of calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI) has been analyzed in cervical and lumbar dorsal root ganglia of untreated and colchicine-treated rats. In addition, lumbar ganglia were examined 2 weeks after transection of the sciatic nerve. The occurrence of CGRP-positive cells in relation to ganglion cells containing substance P-, somatostatin-, galanin-, cholecystokinin (CCK)-, and vasoactive intestinal polypeptide (VIP)/peptide histidine isoleucin (PHI)-LI has been evaluated on consecutive sections as well as using elution-restaining and double-staining techniques. CGRP-LI was observed in many ganglion cells of all sizes ranging in diameter from 15 μm to 65 μm. Thus, this peptide occurs also in the large primary sensory neurons. In contrast to the sensory peptides described to date, CGRP-positive cells constituted up to 50% of all and 70% of the medium-sized neurons, thus being the most frequently occurring peptide in sensory neurons so far encountered. Subpulations of CGRP-positive neurons were shown to contain substance P-, somatostatin-, or galanin-LI and some CGRP-positive neurons contained both substance P- and galanin-LI. In fact, most substance P-, somatostatin- and galanin-positive cell bodies were CGRP-immunoreactive. The coexistence analysis further revealed that galanin and substance P often coexisted and that some cells contained both substance P- and somatostatin-LI, whereas no coexistence between galanin and somatostatin has as yet been seen. VIP/PHI-LI was only shown in a few cells in untreated or colchicine-treated rats. However, after transcetion of the sciatic nerve numerous VIP/PHI-positive cells were observed, some of which also contained CGRP-LI. The present results indicate that a CGRP-like peptide is present in a wide range of primary sensory neurons probably not related to specific sensory modalities. Often this peptide coexists with other biologically active peptides. Taken together these findings suggest that CGRP may have a generalized function.
         datePublished:
         dateModified:
         pageStart:417
         pageEnd:431
         sameAs:https://doi.org/10.1007/BF00218323
         keywords:
            Dorsal root ganglia
            Neuropeptides
            Coexistence
            Immunohistochemistry
            Rat
            Human Genetics
            Proteomics
            Molecular Medicine
         image:
         isPartOf:
            name:Cell and Tissue Research
            issn:
               1432-0878
               0302-766X
            volumeNumber:247
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:G. Ju
               affiliation:
                     name:Karolinska Institutet
                     address:
                        name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Tomas Hökfelt
               affiliation:
                     name:Karolinska Institutet
                     address:
                        name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
                        type:PostalAddress
                     type:Organization
               type:Person
               name:E. Brodin
               affiliation:
                     name:Karolinska Institutet
                     address:
                        name:Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden
                        type:PostalAddress
                     type:Organization
               type:Person
               name:J. Fahrenkrug
               affiliation:
                     name:Bispebjerg Hospital
                     address:
                        name:Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark
                        type:PostalAddress
                     type:Organization
               type:Person
               name:J. A. Fischer
               affiliation:
                     name:University of Zürich
                     address:
                        name:Departments of Orthopedic Surgery and Medicine, University of Zürich, Zürich, Switzerland
                        type:PostalAddress
                     type:Organization
               type:Person
               name:P. Frey
               affiliation:
                     name:Wander Research Institute
                     address:
                        name:Sandoz Research Unit, Wander Research Institute, Bern, Switzerland
                        type:PostalAddress
                     type:Organization
               type:Person
               name:R. P. Elde
               affiliation:
                     name:University of Minnesota
                     address:
                        name:Department of Anatomy, University of Minnesota, Minneapolis, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:J. C. Brown
               affiliation:
                     name:University of British Columbia
                     address:
                        name:Medical Research Council of Canada Regulatory Peptide Group, University of British Columbia, Vancouver, Canada
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Primary sensory neurons of the rat showing calcitonin gene-related peptide immunoreactivity and their relation to substance P-, somatostatin-, galanin-, vasoactive intestinal polypeptide- and cholecystokinin-immunoreactive ganglion cells
      description:By use of the indirect immunofluorescence technique the distribution of calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI) has been analyzed in cervical and lumbar dorsal root ganglia of untreated and colchicine-treated rats. In addition, lumbar ganglia were examined 2 weeks after transection of the sciatic nerve. The occurrence of CGRP-positive cells in relation to ganglion cells containing substance P-, somatostatin-, galanin-, cholecystokinin (CCK)-, and vasoactive intestinal polypeptide (VIP)/peptide histidine isoleucin (PHI)-LI has been evaluated on consecutive sections as well as using elution-restaining and double-staining techniques. CGRP-LI was observed in many ganglion cells of all sizes ranging in diameter from 15 μm to 65 μm. Thus, this peptide occurs also in the large primary sensory neurons. In contrast to the sensory peptides described to date, CGRP-positive cells constituted up to 50% of all and 70% of the medium-sized neurons, thus being the most frequently occurring peptide in sensory neurons so far encountered. Subpulations of CGRP-positive neurons were shown to contain substance P-, somatostatin-, or galanin-LI and some CGRP-positive neurons contained both substance P- and galanin-LI. In fact, most substance P-, somatostatin- and galanin-positive cell bodies were CGRP-immunoreactive. The coexistence analysis further revealed that galanin and substance P often coexisted and that some cells contained both substance P- and somatostatin-LI, whereas no coexistence between galanin and somatostatin has as yet been seen. VIP/PHI-LI was only shown in a few cells in untreated or colchicine-treated rats. However, after transcetion of the sciatic nerve numerous VIP/PHI-positive cells were observed, some of which also contained CGRP-LI. The present results indicate that a CGRP-like peptide is present in a wide range of primary sensory neurons probably not related to specific sensory modalities. Often this peptide coexists with other biologically active peptides. Taken together these findings suggest that CGRP may have a generalized function.
      datePublished:
      dateModified:
      pageStart:417
      pageEnd:431
      sameAs:https://doi.org/10.1007/BF00218323
      keywords:
         Dorsal root ganglia
         Neuropeptides
         Coexistence
         Immunohistochemistry
         Rat
         Human Genetics
         Proteomics
         Molecular Medicine
      image:
      isPartOf:
         name:Cell and Tissue Research
         issn:
            1432-0878
            0302-766X
         volumeNumber:247
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:G. Ju
            affiliation:
                  name:Karolinska Institutet
                  address:
                     name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tomas Hökfelt
            affiliation:
                  name:Karolinska Institutet
                  address:
                     name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
                     type:PostalAddress
                  type:Organization
            type:Person
            name:E. Brodin
            affiliation:
                  name:Karolinska Institutet
                  address:
                     name:Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden
                     type:PostalAddress
                  type:Organization
            type:Person
            name:J. Fahrenkrug
            affiliation:
                  name:Bispebjerg Hospital
                  address:
                     name:Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark
                     type:PostalAddress
                  type:Organization
            type:Person
            name:J. A. Fischer
            affiliation:
                  name:University of Zürich
                  address:
                     name:Departments of Orthopedic Surgery and Medicine, University of Zürich, Zürich, Switzerland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:P. Frey
            affiliation:
                  name:Wander Research Institute
                  address:
                     name:Sandoz Research Unit, Wander Research Institute, Bern, Switzerland
                     type:PostalAddress
                  type:Organization
            type:Person
            name:R. P. Elde
            affiliation:
                  name:University of Minnesota
                  address:
                     name:Department of Anatomy, University of Minnesota, Minneapolis, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:J. C. Brown
            affiliation:
                  name:University of British Columbia
                  address:
                     name:Medical Research Council of Canada Regulatory Peptide Group, University of British Columbia, Vancouver, Canada
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Cell and Tissue Research
      issn:
         1432-0878
         0302-766X
      volumeNumber:247
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Karolinska Institutet
      address:
         name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
         type:PostalAddress
      name:Karolinska Institutet
      address:
         name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
         type:PostalAddress
      name:Karolinska Institutet
      address:
         name:Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden
         type:PostalAddress
      name:Bispebjerg Hospital
      address:
         name:Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark
         type:PostalAddress
      name:University of Zürich
      address:
         name:Departments of Orthopedic Surgery and Medicine, University of Zürich, Zürich, Switzerland
         type:PostalAddress
      name:Wander Research Institute
      address:
         name:Sandoz Research Unit, Wander Research Institute, Bern, Switzerland
         type:PostalAddress
      name:University of Minnesota
      address:
         name:Department of Anatomy, University of Minnesota, Minneapolis, USA
         type:PostalAddress
      name:University of British Columbia
      address:
         name:Medical Research Council of Canada Regulatory Peptide Group, University of British Columbia, Vancouver, Canada
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:G. Ju
      affiliation:
            name:Karolinska Institutet
            address:
               name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
               type:PostalAddress
            type:Organization
      name:Tomas Hökfelt
      affiliation:
            name:Karolinska Institutet
            address:
               name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
               type:PostalAddress
            type:Organization
      name:E. Brodin
      affiliation:
            name:Karolinska Institutet
            address:
               name:Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden
               type:PostalAddress
            type:Organization
      name:J. Fahrenkrug
      affiliation:
            name:Bispebjerg Hospital
            address:
               name:Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark
               type:PostalAddress
            type:Organization
      name:J. A. Fischer
      affiliation:
            name:University of Zürich
            address:
               name:Departments of Orthopedic Surgery and Medicine, University of Zürich, Zürich, Switzerland
               type:PostalAddress
            type:Organization
      name:P. Frey
      affiliation:
            name:Wander Research Institute
            address:
               name:Sandoz Research Unit, Wander Research Institute, Bern, Switzerland
               type:PostalAddress
            type:Organization
      name:R. P. Elde
      affiliation:
            name:University of Minnesota
            address:
               name:Department of Anatomy, University of Minnesota, Minneapolis, USA
               type:PostalAddress
            type:Organization
      name:J. C. Brown
      affiliation:
            name:University of British Columbia
            address:
               name:Medical Research Council of Canada Regulatory Peptide Group, University of British Columbia, Vancouver, Canada
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
      name:Department of Histology, Karolinska Institutet, Stockholm, Sweden
      name:Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden
      name:Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen, Denmark
      name:Departments of Orthopedic Surgery and Medicine, University of Zürich, Zürich, Switzerland
      name:Sandoz Research Unit, Wander Research Institute, Bern, Switzerland
      name:Department of Anatomy, University of Minnesota, Minneapolis, USA
      name:Medical Research Council of Canada Regulatory Peptide Group, University of British Columbia, Vancouver, Canada
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(139)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.91s.

LINK . SPRINGER . COM {}