We are analyzing https://link.springer.com/article/10.1007/bf00205646.

Title:
Clonal analysis and in situ characterization of lymphocytes infiltrating human breast carcinomas | Cancer Immunology, Immunotherapy
Description:
T lymphocytes were isolated from tumor biopsies in 13 patients with breast carcinomas. Immunohistology with monoclonal antibodies confirmed the presence of mononuclear cell infiltrates composed primarily of T lymphocytes in all tumors studied. While the proportion of T lymphocytes expressing the T4 or the T8 surface marker varied from tumor to tumor as determined by morphometric analysis, T8+ cells were more numerous than T4+ cells in 8/12 breast tumors studied. Relatively few T cells (<10% in 11/12 tumors) were in an activated state as judged by the surface expression of HLA-DR antigens or the receptor for interleukin-2 (IL-2). In 1 case 20% of the infiltrating mononuclear cells were expressing the IL-2 receptor. The tumor infiltrating lymphocytes (TIL) recovered from 10 tumors were cloned in a microculture system that permits proliferation of nearly 100% of normal peripheral blood T lymphocytes (PBL-T). In contrast to normal and autologous PBL-T, frequencies of proliferating T lymphocyte precursors (PTL-P) were depressed (<0.01) in 7/10 TIL preparations indicating a decreased responsiveness of TIL to phytohemagglutinin at the single-cell level. The frequency of PTL-P was noticeably higher in 2 cases (0.03 and 0.09) and close to normal in 1 case (0.39). A total of 170 clones were expanded in vitro and analyzed for different functional capabilities. Most of these clones expressed the T4+/T8-phenotype (73%) and strikingly 53% of these T4+/T8− clones were cytolytic in a lectin-dependent assay, a functional subset which is uncommon among normal PBL-T. Some clones (10%) lysed allogeneic breast tumor cells (MCF7). Only 15% of the clones displayed natural killer activity. Among the cytolytic clones, 17 of 31 tested were also IL-2 producers irrespective of the T4 or T8 phenotype. Our results show that human mammary carcinomas contain many infiltrating T cells with cytolytic potential. Interestingly, among the proliferating cytolytic T cell clones (56% of the microcultures), many expressed the T4+/T8− phenotype. These findings may indicate that the in situ cytolytic reaction (against unknown antigens) is associated preferentially with class II antigens.
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Keywords {🔍}

google, scholar, cancer, human, cells, lymphocytes, breast, analysis, cell, infiltrating, moretta, tumor, article, tumors, clonal, cytolytic, immunol, blood, clones, activity, cytotoxic, int, characterization, antigens, functional, patients, lymphocyte, access, exp, med, carcinoma, privacy, cookies, content, research, carcinomas, whiteside, miescher, hurlimann, surface, peripheral, autologous, melanoma, vose, data, information, publish, search, situ, fliedner,

Topics {✒️}

interleukin-2-dependent t-cell cultures month download article/chapter human breast carcinoma human tumour-lymphocyte interaction human tumor-infiltrating lymphocytes inflammatory cellular infiltrate t-cell-mediated cytotoxicity cytotoxic t-lymphocyte response cells t-cell lymphoma t-cell clones capable tumour-reactive lymphocytes stimulated breast cancer infiltrates nk-cell activity human mammary carcinomas human breast cancer tumor infiltrating lymphocytes tumor-infiltrating lymphocytes infiltrating mononuclear cells breast cancer microenvironment privacy choices/manage cookies invasive breast cancer human ovarian tumors express cytolytic activity lymphocyte precursors breast carcinomas cell-mediated immunity human malignant tumors malignant breast disease full article pdf lectin-dependent assay depressed immune competence cancer research campaign single-cell level cell growth factor human leukocyte antigens von fliedner cloned human cytotoxic article cancer immunology breast cancer patients host cell populations cell surface antigens hla-dr antigens defined clinical stages cells cd4-positive lymph-node lymphocytes european economic area check access endogenous enzyme hexosaminidase del prete gf rossi-ferrini pl

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         headline:Clonal analysis and in situ characterization of lymphocytes infiltrating human breast carcinomas
         description:T lymphocytes were isolated from tumor biopsies in 13 patients with breast carcinomas. Immunohistology with monoclonal antibodies confirmed the presence of mononuclear cell infiltrates composed primarily of T lymphocytes in all tumors studied. While the proportion of T lymphocytes expressing the T4 or the T8 surface marker varied from tumor to tumor as determined by morphometric analysis, T8+ cells were more numerous than T4+ cells in 8/12 breast tumors studied. Relatively few T cells (<10% in 11/12 tumors) were in an activated state as judged by the surface expression of HLA-DR antigens or the receptor for interleukin-2 (IL-2). In 1 case 20% of the infiltrating mononuclear cells were expressing the IL-2 receptor. The tumor infiltrating lymphocytes (TIL) recovered from 10 tumors were cloned in a microculture system that permits proliferation of nearly 100% of normal peripheral blood T lymphocytes (PBL-T). In contrast to normal and autologous PBL-T, frequencies of proliferating T lymphocyte precursors (PTL-P) were depressed (<0.01) in 7/10 TIL preparations indicating a decreased responsiveness of TIL to phytohemagglutinin at the single-cell level. The frequency of PTL-P was noticeably higher in 2 cases (0.03 and 0.09) and close to normal in 1 case (0.39). A total of 170 clones were expanded in vitro and analyzed for different functional capabilities. Most of these clones expressed the T4+/T8-phenotype (73%) and strikingly 53% of these T4+/T8− clones were cytolytic in a lectin-dependent assay, a functional subset which is uncommon among normal PBL-T. Some clones (10%) lysed allogeneic breast tumor cells (MCF7). Only 15% of the clones displayed natural killer activity. Among the cytolytic clones, 17 of 31 tested were also IL-2 producers irrespective of the T4 or T8 phenotype. Our results show that human mammary carcinomas contain many infiltrating T cells with cytolytic potential. Interestingly, among the proliferating cytolytic T cell clones (56% of the microcultures), many expressed the T4+/T8− phenotype. These findings may indicate that the in situ cytolytic reaction (against unknown antigens) is associated preferentially with class II antigens.
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      headline:Clonal analysis and in situ characterization of lymphocytes infiltrating human breast carcinomas
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      dateModified:
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      pageEnd:178
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         Tumor Infiltrate Lymphocyte
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         Immunology
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