We are analyzing https://link.springer.com/article/10.1007/bf00021187.

Title:
Promoters from kin1 and cor6.6, two homologous Arabidopsis thaliana genes: transcriptional regulation and gene expression induced by low temperature, ABA, osmoticum and dehydration | Plant Molecular Biology
Description:
The Arabidopsis thaliana genes kin1 and cor6.6 belong to the same family and were expressed at higher levels following low temperature and ABA treatments. In an attempt to elucidate the mechanism of gene regulation by low temperature, the relationship between low-temperature- and abscisic acid (ABA)-induced gene expression and possible differential expression of the two genes, we have cloned a 5.3 kb genomic fragment harboring kin1 and cor6.6 and their respective 5′ sequences. The putative promoters of both genes were fused to the β-glucuronidase (GUS) coding sequence and GUS expression was analysed in transgenic tobacco and Arabidopsis plants. The cor6.6 promoter produced a higher basal level of expression than the kin1 promoter in transgenic tobacco. Enzyme assays of inducible GUS activity in transgenic Arabidopsis and tobacco plants showed that GUS activity directed by both kin1 and cor6.6 promoters was significantly induced by ABA, dehydration and osmoticum, but not by low temperature. Northern analysis revealed, in contrast, that GUS mRNA was significantly induced in these transgenic plants by low temperature. Further analysis showed that, at low temperature, GUS protein synthesis from the induced GUS mRNA was inhibited. Together these results reveal induction of kin1 and cor6.6 transcription by low temperature, exogenous ABA and dehydration. However, low-temperature expression is dramatically reduced at the translational level.
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Keywords {🔍}

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Topics {✒️}

month download article/chapter abscisic acid-responsive promoter agrobacterium tumefaciens-mediated transformation plant molecular biology desiccation-responsive rd29 gene low-temperature-induced gene low-temperature-induced genes abscisic acid-responsive sequences expressed β-tubulin genes aba-inducible arabidopsis gene cold-regulated gene expression molecular biology aba-regulated gene expression national research council privacy choices/manage cookies full article pdf abscisic acid contents rab-related gene cold-regulated genes plant cold hardiness low temperature-induced potato cold acclimation cold acclimation process aba-induced genes 193 bp promoter region related subjects assaying chimeric genes induced gene expression gene expression induced preparing plant samples cdna sequence analysis dna sequence analysis distinct regulatory pathways rice rab gene cis-acting element fish antifreeze proteins cis-elements responsible cis-acting elements inducible gus activity transgenic arabidopsis arabidopsis thaliana cor15b european economic area results reveal induction ho t-hd freezing stress tolerance high-fidelity amplification spinach gene responsive mary loewen & adrian northern analysis revealed induced gus mrna

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         description:The Arabidopsis thaliana genes kin1 and cor6.6 belong to the same family and were expressed at higher levels following low temperature and ABA treatments. In an attempt to elucidate the mechanism of gene regulation by low temperature, the relationship between low-temperature- and abscisic acid (ABA)-induced gene expression and possible differential expression of the two genes, we have cloned a 5.3 kb genomic fragment harboring kin1 and cor6.6 and their respective 5′ sequences. The putative promoters of both genes were fused to the β-glucuronidase (GUS) coding sequence and GUS expression was analysed in transgenic tobacco and Arabidopsis plants. The cor6.6 promoter produced a higher basal level of expression than the kin1 promoter in transgenic tobacco. Enzyme assays of inducible GUS activity in transgenic Arabidopsis and tobacco plants showed that GUS activity directed by both kin1 and cor6.6 promoters was significantly induced by ABA, dehydration and osmoticum, but not by low temperature. Northern analysis revealed, in contrast, that GUS mRNA was significantly induced in these transgenic plants by low temperature. Further analysis showed that, at low temperature, GUS protein synthesis from the induced GUS mRNA was inhibited. Together these results reveal induction of kin1 and cor6.6 transcription by low temperature, exogenous ABA and dehydration. However, low-temperature expression is dramatically reduced at the translational level.
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            cold acclimation
            low temperature-induced genes
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      headline:Promoters from kin1 and cor6.6, two homologous Arabidopsis thaliana genes: transcriptional regulation and gene expression induced by low temperature, ABA, osmoticum and dehydration
      description:The Arabidopsis thaliana genes kin1 and cor6.6 belong to the same family and were expressed at higher levels following low temperature and ABA treatments. In an attempt to elucidate the mechanism of gene regulation by low temperature, the relationship between low-temperature- and abscisic acid (ABA)-induced gene expression and possible differential expression of the two genes, we have cloned a 5.3 kb genomic fragment harboring kin1 and cor6.6 and their respective 5′ sequences. The putative promoters of both genes were fused to the β-glucuronidase (GUS) coding sequence and GUS expression was analysed in transgenic tobacco and Arabidopsis plants. The cor6.6 promoter produced a higher basal level of expression than the kin1 promoter in transgenic tobacco. Enzyme assays of inducible GUS activity in transgenic Arabidopsis and tobacco plants showed that GUS activity directed by both kin1 and cor6.6 promoters was significantly induced by ABA, dehydration and osmoticum, but not by low temperature. Northern analysis revealed, in contrast, that GUS mRNA was significantly induced in these transgenic plants by low temperature. Further analysis showed that, at low temperature, GUS protein synthesis from the induced GUS mRNA was inhibited. Together these results reveal induction of kin1 and cor6.6 transcription by low temperature, exogenous ABA and dehydration. However, low-temperature expression is dramatically reduced at the translational level.
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      dateModified:
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      pageEnd:617
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         abscisic acid
          Arabidopsis
         cold acclimation
         low temperature-induced genes
         promoter
         transgenic
         Plant Sciences
         Biochemistry
         general
         Plant Pathology
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