We are analyzing https://link.springer.com/article/10.1007/bf01703446.

Title:
Direct in vivo biotinylation of erythrocytes as an assay for red cell survival studies | Annals of Hematology
Description:
Direct in vivo labeling of erythrocytes with biotin is shown as a method for estimation of red cell survival as well as of enrichment of young or aged erythrocytes. Two succinimide esters (biotin-N-hydroxysuccinimide ester [BNHS], caproylamidobiotin-N-hydroxysuccinimide ester [C-BNHS] were used for biotin labeling of erythrocytes. With improved syntheses, pure BNHS (mp, 212°–214° C) and the spacered intermediate for C-BNHS, 6-(biotinylamide) hexanoate (mp, 225°–226° C) were obtained in an overall yield of 86%; the yield of C-BNHS (mp, 167°–169° C) was 68%. When three doses of 1 mg C-BNHS are injected intravenously into mice at 24-h intervals, all the red cells are biotin labeled. The rate of red cell production as well as the life span of red cells can be measured without any effect on erythropoiesis or damage by red cells in vitro. The survival curve seems to be linear, with 2.5%–3.3% disappearance of biotin-labeled red cells daily. In mice, in vivo biotin labeling avoids damaging red cells by in vitro procedures and does not influence the steady state of erythropoiesis by hypertransfusion. Therefore, in vivo biotin labeling is a very useful method for determining red cell survival time in small animals.
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Keywords {🔍}

google, scholar, red, article, cells, cell, survival, blood, vivo, erythrocytes, biotin, labeling, access, privacy, cookies, content, studies, mice, information, publish, search, thierfelder, method, cbnhs, vitro, institut, gsf, frg, analysis, data, log, journal, research, hematology, direct, hoffmannfezer, maschke, zeitler, gais, life, effect, erythropoiesis, state, animals, factor, hematopoietic, open, system, discover, biological,

Topics {✒️}

caproylamidobiotin-n-hydroxysuccinimide ester [c-bnhs] biotin-n-hydroxysuccinimide ester [bnhs] month download article/chapter red cell survival age-structured population model biological studies red cell production erythropoiesis-inhibiting factor related subjects avidin-biotin system privacy choices/manage cookies full article pdf hematopoietic stem cells vivo biotin labeling biotin-labeled cells 1 mg c-bnhs article hoffmann-fezer transfused blood corpuscles european economic area scope submit manuscript immune cellular agglutination percoll/hypaque results erythrocyte iron incorporation conditions privacy policy check access instant access article annals accepting optional cookies hematopoietic cells vivo survival biotin labeling main content log journal finder publish red cells survival curve biotin labeled usage analysis vivo labeling article log c-bnhs article cite van furth dale gl privacy policy personal data books a pure bnhs steady state blood transfusion optional cookies

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      headline:Direct in vivo biotinylation of erythrocytes as an assay for red cell survival studies
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      dateModified:
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