We are analyzing https://link.springer.com/article/10.1007/bf01309671.

Title:
Physicochemical properties and cytopathogenicity of an adenovirus-like agent isolated from corn snake (Elaphe guttata) | Archives of Virology
Description:
A virus isolated from the internal organs of a moribund corn snake (Elaphe guttata) replicated in reptilian cell cultures (IgH-2, TH-1 cells) between 10 and 30°C. Highest infectivity titers of 105.5 TCID50/ml were obtained in IgH-2 cells at 25°C. Infected IgH-2 cells showed the development of three morphologically different intranuclear inclusion bodies. During viral assembly the particles formed typical crystalline aggregates in the nucleus. About 64 h after infection progressive desintegration of the nuclear membrane was evident and virus particles were released into the cytoplasm. Different fish cell lines (CLC, CHSE-214, BF-2, PG, RTG-2) were not capable of propagating the virus. The DNA containing agent proved to be stable at pH 3, more or less at pH 12 and to treatment with chloroform, but it was rapidly inactivated at 56°C. Electron microscopy revealed nonenveloped icosahedral particles with a diameter of 65–70 nm.
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Keywords {🔍}

google, scholar, cell, cells, article, virus, ahne, med, adenovirus, viruses, isolated, infected, line, exp, privacy, cookies, content, virology, access, replication, reptiles, publish, search, adenoviruslike, cultures, viral, fish, electron, adenoviruses, pathol, vitro, vet, eds, jacobson, springer, data, information, log, journal, research, archives, physicochemical, cytopathogenicity, agent, corn, snake, elaphe, guttata, juhasz, igh,

Topics {✒️}

month download article/chapter related subjects infectious virus privacy choices/manage cookies full article pdf reptilian cell cultures icosahedral cytoplasmic deoxyribovirus virus particles check access instant access primary cell cultures infected kb cells fish cell lines cultured bovine cells continuous cell line permanent cell line fish cell cultures la cells infected hela cells infected established eurythermic line european economic area original papers published scope submit manuscript highest infectivity titers intranuclear inclusion bodies 4th international colloquium deutsche veterinärmedizinische gesellschaft denny fw jr box turtleterrapene carolina normal common carp gastrointestinal disease physicochemical properties electron microscope study conditions privacy policy romanowsky-giemsa effect accepting optional cookies virus isolated lucké tumour isolated cell biol 13 moribund corn snake infection progressive desintegration journal finder publish article archives article log cell line exp med 112 exp med 104 viral replication compartments electron microscopy chloroform

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