We are analyzing https://link.springer.com/article/10.1007/bf00331828.

Title:
Polytene chromosomes of Oxytricha: Biochemical and morphological changes during macronuclear development in a ciliated protozoan | Chromosoma
Description:
After conjugation in the ciliated protozoan, Oxytricha, polytene chromosomes are formed during the development of a macronucleus from a micronucleus. Here we report a microscopic study of these chromosomes and an analysis of their DNA. The polytene chromosomes of Oxytricha bear a strong morphological resemblance to the polytene chromosomes of the Dipteran salivary gland. The nucleus of a developing macronuclear anlage contains 120±2 polytene chromosomes and each chromosome has an average of 81 bands; a total of about 10,000 bands per nucleus. At a later stage in development, the number of bands per chromosome is reduced by a factor of four, presumably due to fusion of adjacent bands. The polytene chromosomes then break up into their constituent bands, each of which is encased in a vesicle. There are about 2,700 vesicles per nucleus. — During the growth of polytene chromosomes, there is a change in the relative proportion of sequences in the DNA. The DNA from polytene nuclei has a buoyant density of 1.695 g/cc, significantly lighter than the density of the original micronuclear DNA (1.698 g/cc to 1.702 g/cc). We interpret this buoyant density change to be the result of differential replication of DNA sequences during polytene chromosome growth. A second change in DNA composition occurs after the polytene stage of development, shown by a shift in buoyant density to 1.701 g/cc in the DNA of the mature macronucleus. During this second process, the molecular weight of the DNA is reduced from greater than 50×106 daltons to about 2×106 daltons.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Non-Profit & Charity

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure how the site profits.

While many websites aim to make money, others are created to share knowledge or showcase creativity. People build websites for various reasons. This could be one of them. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

google, scholar, dna, polytene, chromosomes, article, chromosoma, chromosome, stylonychia, macronuclear, development, ciliated, macronucleus, protozoa, biol, mytilus, berl, privacy, cookies, content, change, oxytricha, protozoan, lauth, bands, nature, ammermann, prescott, analysis, information, publish, search, morphological, spear, sequences, density, gcc, access, drosophila, cell, proc, nat, acad, sci, wash, data, log, journal, research, microscopic,

Topics {✒️}

dipteran salivary gland month download article/chapter relative proportion developmental biology subchromosomal-size dna molecules microscopic study stylonychia mytilus exconjugants privacy choices/manage cookies full article pdf strong morphological resemblance related subjects dna breakdown products amplified ribosomal dnas von berger article chromosoma aims stylonychia mytilus european economic area mitochondrial deoxyribonucleic acid nucleic acid metabolism conditions privacy policy developing macronuclear anlage inverted repetitious sequences polytene chromosome dna buoyant density change accepting optional cookies polytene chromosome growth check access instant access ciliated protozoan published electron microscopic main content log original micronuclear dna mitochondrial dna replication repetitive dna sequences journal finder publish dna sequence diminution gene diminution dna composition occurs article log article spear polytene chromosome article cite privacy policy personal data books a adjacent bands polytene chromosomes 120±2 polytene chromosomes stylonychia spec stylonychia sp

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Polytene chromosomes of Oxytricha: Biochemical and morphological changes during macronuclear development in a ciliated protozoan
         description:After conjugation in the ciliated protozoan, Oxytricha, polytene chromosomes are formed during the development of a macronucleus from a micronucleus. Here we report a microscopic study of these chromosomes and an analysis of their DNA. The polytene chromosomes of Oxytricha bear a strong morphological resemblance to the polytene chromosomes of the Dipteran salivary gland. The nucleus of a developing macronuclear anlage contains 120±2 polytene chromosomes and each chromosome has an average of 81 bands; a total of about 10,000 bands per nucleus. At a later stage in development, the number of bands per chromosome is reduced by a factor of four, presumably due to fusion of adjacent bands. The polytene chromosomes then break up into their constituent bands, each of which is encased in a vesicle. There are about 2,700 vesicles per nucleus. — During the growth of polytene chromosomes, there is a change in the relative proportion of sequences in the DNA. The DNA from polytene nuclei has a buoyant density of 1.695 g/cc, significantly lighter than the density of the original micronuclear DNA (1.698 g/cc to 1.702 g/cc). We interpret this buoyant density change to be the result of differential replication of DNA sequences during polytene chromosome growth. A second change in DNA composition occurs after the polytene stage of development, shown by a shift in buoyant density to 1.701 g/cc in the DNA of the mature macronucleus. During this second process, the molecular weight of the DNA is reduced from greater than 50×106 daltons to about 2×106 daltons.
         datePublished:
         dateModified:
         pageStart:1
         pageEnd:13
         sameAs:https://doi.org/10.1007/BF00331828
         keywords:
            Morphological Change
            Salivary Gland
            Developmental Biology
            Microscopic Study
            Relative Proportion
            Cell Biology
            Biochemistry
            general
            Human Genetics
            Animal Genetics and Genomics
            Eukaryotic Microbiology
         image:
         isPartOf:
            name:Chromosoma
            issn:
               1432-0886
               0009-5915
            volumeNumber:54
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Brian B. Spear
               affiliation:
                     name:University of Colorado
                     address:
                        name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Marlene R. Lauth
               affiliation:
                     name:University of Colorado
                     address:
                        name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Polytene chromosomes of Oxytricha: Biochemical and morphological changes during macronuclear development in a ciliated protozoan
      description:After conjugation in the ciliated protozoan, Oxytricha, polytene chromosomes are formed during the development of a macronucleus from a micronucleus. Here we report a microscopic study of these chromosomes and an analysis of their DNA. The polytene chromosomes of Oxytricha bear a strong morphological resemblance to the polytene chromosomes of the Dipteran salivary gland. The nucleus of a developing macronuclear anlage contains 120±2 polytene chromosomes and each chromosome has an average of 81 bands; a total of about 10,000 bands per nucleus. At a later stage in development, the number of bands per chromosome is reduced by a factor of four, presumably due to fusion of adjacent bands. The polytene chromosomes then break up into their constituent bands, each of which is encased in a vesicle. There are about 2,700 vesicles per nucleus. — During the growth of polytene chromosomes, there is a change in the relative proportion of sequences in the DNA. The DNA from polytene nuclei has a buoyant density of 1.695 g/cc, significantly lighter than the density of the original micronuclear DNA (1.698 g/cc to 1.702 g/cc). We interpret this buoyant density change to be the result of differential replication of DNA sequences during polytene chromosome growth. A second change in DNA composition occurs after the polytene stage of development, shown by a shift in buoyant density to 1.701 g/cc in the DNA of the mature macronucleus. During this second process, the molecular weight of the DNA is reduced from greater than 50×106 daltons to about 2×106 daltons.
      datePublished:
      dateModified:
      pageStart:1
      pageEnd:13
      sameAs:https://doi.org/10.1007/BF00331828
      keywords:
         Morphological Change
         Salivary Gland
         Developmental Biology
         Microscopic Study
         Relative Proportion
         Cell Biology
         Biochemistry
         general
         Human Genetics
         Animal Genetics and Genomics
         Eukaryotic Microbiology
      image:
      isPartOf:
         name:Chromosoma
         issn:
            1432-0886
            0009-5915
         volumeNumber:54
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Brian B. Spear
            affiliation:
                  name:University of Colorado
                  address:
                     name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Marlene R. Lauth
            affiliation:
                  name:University of Colorado
                  address:
                     name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Chromosoma
      issn:
         1432-0886
         0009-5915
      volumeNumber:54
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Colorado
      address:
         name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
         type:PostalAddress
      name:University of Colorado
      address:
         name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Brian B. Spear
      affiliation:
            name:University of Colorado
            address:
               name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
               type:PostalAddress
            type:Organization
      name:Marlene R. Lauth
      affiliation:
            name:University of Colorado
            address:
               name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
      name:Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(56)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.44s.

LINK . SPRINGER . COM {}