We are analyzing https://link.springer.com/article/10.1007/bf00330411.

Title:
Unequal distribution of DNA in the macronuclear division of the ciliate Euplotes eurystomus | Chromosoma
Description:
During asexual fission in the ciliate Euplotes eurystomus, the macronucleus divides amitotically. The macronucleus was found to divide unequally, yielding sister pairs having a mean difference in DNA content of 11.6%. DNA content was determined by the Feulgen reaction using a fluorescent Schiffs reagent, and measuring fluorescence by cytophotometry. Variability in macronuclear DNA content was also examined in randomly-paired non-sister cells, and found to be greater than in sister cells. This greater variability could be due to accumulation of differences over a number of divisions, or to interclonal differences in equality of division. Two categories of non-sister cells were examined: recently divided, and “parents” constructed by averaging the DNA contents of progeny. Both showed similar variability in quantity of macronuclear DNA. The fact that cells surviving to divide showed no less variability in amount of DNA than cells immediately after division suggests that extremes in amounts of DNA resulting from unequal division are not selected against.
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Keywords {🔍}

google, scholar, dna, macronuclear, cell, macronucleus, article, content, division, euplotes, ciliate, chromosoma, tetrahymena, prescott, variability, cells, privacy, cookies, research, distribution, access, stylonychia, res, publish, search, unequal, eurystomus, development, berl, quantitative, york, press, function, analysis, data, information, log, journal, witt, sister, feulgen, similar, discover, genet, ciliates, mytilus, protozoan, exp, pyriformis, sci,

Topics {✒️}

month download article/chapter subchromosomal-size dna molecules showed similar variability related subjects random segregation models transmission electron microscopy unequal distribution privacy choices/manage cookies full article pdf measuring fluorescence yielding sister pairs article chromosoma aims european economic area fluorescent schiffs reagent gene expression expression small aquatic organisms inverted repetitious sequences similar content check access instant access conditions privacy policy dna metabolism ciliated protozoan euplotes macronucleus divides amitotically paramecium functionally diploid unequal division optimal feulgen hydrolysis quantitative cytochemical studies macronuclear gene copies total macronuclear protein accepting optional cookies asymmetric cell division main content log ciliate euplotes eurystomus macronuclear dna content random distribution cell division cycle ciliates stylonychia mytilus journal finder publish ciliate tetrahymena pyriformis greater variability von article witt witt rights sister cells sister cells article log article cite privacy policy personal data

Questions {❓}

Are macronuclear subunits in Paramecium functionally diploid?

Schema {🗺️}

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         headline:Unequal distribution of DNA in the macronuclear division of the ciliate Euplotes eurystomus
         description:During asexual fission in the ciliate Euplotes eurystomus, the macronucleus divides amitotically. The macronucleus was found to divide unequally, yielding sister pairs having a mean difference in DNA content of 11.6%. DNA content was determined by the Feulgen reaction using a fluorescent Schiffs reagent, and measuring fluorescence by cytophotometry. Variability in macronuclear DNA content was also examined in randomly-paired non-sister cells, and found to be greater than in sister cells. This greater variability could be due to accumulation of differences over a number of divisions, or to interclonal differences in equality of division. Two categories of non-sister cells were examined: recently divided, and “parents” constructed by averaging the DNA contents of progeny. Both showed similar variability in quantity of macronuclear DNA. The fact that cells surviving to divide showed no less variability in amount of DNA than cells immediately after division suggests that extremes in amounts of DNA resulting from unequal division are not selected against.
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            Eukaryotic Microbiology
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      headline:Unequal distribution of DNA in the macronuclear division of the ciliate Euplotes eurystomus
      description:During asexual fission in the ciliate Euplotes eurystomus, the macronucleus divides amitotically. The macronucleus was found to divide unequally, yielding sister pairs having a mean difference in DNA content of 11.6%. DNA content was determined by the Feulgen reaction using a fluorescent Schiffs reagent, and measuring fluorescence by cytophotometry. Variability in macronuclear DNA content was also examined in randomly-paired non-sister cells, and found to be greater than in sister cells. This greater variability could be due to accumulation of differences over a number of divisions, or to interclonal differences in equality of division. Two categories of non-sister cells were examined: recently divided, and “parents” constructed by averaging the DNA contents of progeny. Both showed similar variability in quantity of macronuclear DNA. The fact that cells surviving to divide showed no less variability in amount of DNA than cells immediately after division suggests that extremes in amounts of DNA resulting from unequal division are not selected against.
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         Great Variability
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         Similar Variability
         Sister Cell
         Cell Biology
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         general
         Human Genetics
         Animal Genetics and Genomics
         Eukaryotic Microbiology
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