We are analyzing https://link.springer.com/article/10.1007/bf00284071.

Title:
Chromosome-sized DNA molecules from Drosophila | Chromosoma
Description:
Measurements of viscoelastic retardation times of detergent-Pronase lysates of Drosophila cells demonstrated the presence of large numbers of DNA molecules of a size commensurate with that of the chromosomes. The values estimated from the retardation times for the molecular weights of the largest molecules ranged from about 20×109 to 80×109 daltons depending on the species of Drosophila. The molecular weights of the DNA molecules were independent of the metaphase shapes (i.e., metacentric or submetacentric), but were proportional to the DNA contents of the chromosomes in the case of translocations or deletions. It was concluded, therefore, that the DNA molecules must run the length of the chromosome and cannot be discontinuous at the centromere. When compared with the values of the DNA contents of Drosophila chromosomes determined by other methods, the results were consistent with the model of one, or possibly two, DNA molecules per chromosome; the simplest conclusion, that there is only one DNA molecule per chromosome (for simple chromosomes), rests on a long extrapolation of an empirical relation between retardation time and molecular weight, but is also favored by indirect evidence. Further possibilities which could not be excluded were that the large DNA molecules contained Pronase-resistant, non-DNA links, or that a fraction of smaller DNA molecules might also have been present in the chromosomes. Chromosome-sized DNA molecules were obtained almost quantitatively from unsynchronized cultured cells, suggesting that the size of the chromosomal DNA is conserved throughout much of the cell cycle. The molecules were stable for periods of up to several days at 50° C in solutions containing detergent, Pronase, and EDTA.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com might have a hidden revenue stream, but it's not something we can detect.

Keywords {🔍}

dna, google, scholar, biol, drosophila, chromosomes, molec, molecules, article, molecular, chromosome, proc, content, zimm, nat, acad, sci, wash, melanogaster, chromosoma, cell, access, coli, acid, press, sedimentation, privacy, cookies, kavenoff, retardation, cells, deoxyribonucleic, structure, publish, research, search, measurements, times, size, weight, chromosomal, organization, open, escherichia, york, autoradiography, replication, viscosity, data, information,

Topics {✒️}

chromosome-sized dna molecules month download article/chapter random double-strand breaks single-stranded t7 dna indirect evidence polytene chromosome dna related subjects nucleic acid research privacy choices/manage cookies cell cycle full article pdf article chromosoma aims drosophila melanogaster evidence dna-binding protein single dna molecules hela cell dna bacterial chromosome largest molecules ranged smaller dna molecules molecular weight european economic area double-stranded model sodium dodecyl sulfate gel-forming properties tritium-labeled thymidine vicia faba chromosomes dna-cellulose chromatography interspersed spacer dna bacterial dna molecules conditions privacy policy chromosome structure methods simple salt concentration t5 bacteriophage mutants individual metaphase chromosomes dna-binding proteins unsynchronized cultured cells chinese hamster cells detergent-pronase lysates check access instant access chromosomal dna fibers accepting optional cookies article kavenoff interphase nuclei compared nucleotide sequence diversity enzyme digestion studies measuring retardation times drosophila chromosomes determined drosophila cells demonstrated

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Chromosome-sized DNA molecules from Drosophila
         description:Measurements of viscoelastic retardation times of detergent-Pronase lysates of Drosophila cells demonstrated the presence of large numbers of DNA molecules of a size commensurate with that of the chromosomes. The values estimated from the retardation times for the molecular weights of the largest molecules ranged from about 20×109 to 80×109 daltons depending on the species of Drosophila. The molecular weights of the DNA molecules were independent of the metaphase shapes (i.e., metacentric or submetacentric), but were proportional to the DNA contents of the chromosomes in the case of translocations or deletions. It was concluded, therefore, that the DNA molecules must run the length of the chromosome and cannot be discontinuous at the centromere. When compared with the values of the DNA contents of Drosophila chromosomes determined by other methods, the results were consistent with the model of one, or possibly two, DNA molecules per chromosome; the simplest conclusion, that there is only one DNA molecule per chromosome (for simple chromosomes), rests on a long extrapolation of an empirical relation between retardation time and molecular weight, but is also favored by indirect evidence. Further possibilities which could not be excluded were that the large DNA molecules contained Pronase-resistant, non-DNA links, or that a fraction of smaller DNA molecules might also have been present in the chromosomes. Chromosome-sized DNA molecules were obtained almost quantitatively from unsynchronized cultured cells, suggesting that the size of the chromosomal DNA is conserved throughout much of the cell cycle. The molecules were stable for periods of up to several days at 50° C in solutions containing detergent, Pronase, and EDTA.
         datePublished:
         dateModified:
         pageStart:1
         pageEnd:27
         sameAs:https://doi.org/10.1007/BF00284071
         keywords:
            Molecular Weight
            Cell Cycle
            EDTA
            Developmental Biology
            Indirect Evidence
            Cell Biology
            Biochemistry
            general
            Human Genetics
            Animal Genetics and Genomics
            Eukaryotic Microbiology
         image:
         isPartOf:
            name:Chromosoma
            issn:
               1432-0886
               0009-5915
            volumeNumber:41
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Ruth Kavenoff
               affiliation:
                     name:University of California
                     address:
                        name:Department of Chemistry, Revelle College, University of California, San Diego
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Bruno H. Zimm
               affiliation:
                     name:University of California
                     address:
                        name:Department of Chemistry, Revelle College, University of California, San Diego
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Chromosome-sized DNA molecules from Drosophila
      description:Measurements of viscoelastic retardation times of detergent-Pronase lysates of Drosophila cells demonstrated the presence of large numbers of DNA molecules of a size commensurate with that of the chromosomes. The values estimated from the retardation times for the molecular weights of the largest molecules ranged from about 20×109 to 80×109 daltons depending on the species of Drosophila. The molecular weights of the DNA molecules were independent of the metaphase shapes (i.e., metacentric or submetacentric), but were proportional to the DNA contents of the chromosomes in the case of translocations or deletions. It was concluded, therefore, that the DNA molecules must run the length of the chromosome and cannot be discontinuous at the centromere. When compared with the values of the DNA contents of Drosophila chromosomes determined by other methods, the results were consistent with the model of one, or possibly two, DNA molecules per chromosome; the simplest conclusion, that there is only one DNA molecule per chromosome (for simple chromosomes), rests on a long extrapolation of an empirical relation between retardation time and molecular weight, but is also favored by indirect evidence. Further possibilities which could not be excluded were that the large DNA molecules contained Pronase-resistant, non-DNA links, or that a fraction of smaller DNA molecules might also have been present in the chromosomes. Chromosome-sized DNA molecules were obtained almost quantitatively from unsynchronized cultured cells, suggesting that the size of the chromosomal DNA is conserved throughout much of the cell cycle. The molecules were stable for periods of up to several days at 50° C in solutions containing detergent, Pronase, and EDTA.
      datePublished:
      dateModified:
      pageStart:1
      pageEnd:27
      sameAs:https://doi.org/10.1007/BF00284071
      keywords:
         Molecular Weight
         Cell Cycle
         EDTA
         Developmental Biology
         Indirect Evidence
         Cell Biology
         Biochemistry
         general
         Human Genetics
         Animal Genetics and Genomics
         Eukaryotic Microbiology
      image:
      isPartOf:
         name:Chromosoma
         issn:
            1432-0886
            0009-5915
         volumeNumber:41
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Ruth Kavenoff
            affiliation:
                  name:University of California
                  address:
                     name:Department of Chemistry, Revelle College, University of California, San Diego
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Bruno H. Zimm
            affiliation:
                  name:University of California
                  address:
                     name:Department of Chemistry, Revelle College, University of California, San Diego
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Chromosoma
      issn:
         1432-0886
         0009-5915
      volumeNumber:41
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of California
      address:
         name:Department of Chemistry, Revelle College, University of California, San Diego
         type:PostalAddress
      name:University of California
      address:
         name:Department of Chemistry, Revelle College, University of California, San Diego
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Ruth Kavenoff
      affiliation:
            name:University of California
            address:
               name:Department of Chemistry, Revelle College, University of California, San Diego
               type:PostalAddress
            type:Organization
      name:Bruno H. Zimm
      affiliation:
            name:University of California
            address:
               name:Department of Chemistry, Revelle College, University of California, San Diego
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Chemistry, Revelle College, University of California, San Diego
      name:Department of Chemistry, Revelle College, University of California, San Diego
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(76)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.77s.

LINK . SPRINGER . COM {}