We are analyzing https://link.springer.com/article/10.1007/bf00283386.

Title:
The development of the macronucleus in the ciliated protozoan Stylonychia mytilus | Chromosoma
Description:
Ciliated protozoa are characterized by generative micronuclei and vegetative polyploid macronuclei. Micronuclei of Stylonychia mytilus contain 1 600 times as much DNA per haploid genome as E. coli. Most of this DNA is shown to be repetitive. The development of the macronucleus involves, as demonstrated by cytology, only 1/3 of the chromosomes which in a first replication phase are polytenized in probably 5 replication steps and appear as giant chromosomes. At this developmental stage considerable amounts of repetitive DNA are still present in the chromosomes. During the subsequent disintegration phase more than 90% of the DNA are eliminated from the macronucleus anlage. The remainder is further replicated five times and composes the final macronucleus. Since this DNA reassociates with a reaction rate almost identical to an ideal second order reaction its kinetic complexity can be determined by comparison with the kinetic complexity of E. coli DNA. Macronuclear DNA reassociates with a kinetic complexity of 26 times the kinetic complexity of E. coli DNA (corrected for GC content) which indicates that macronuclear DNA sequences exist at a ploidy level of 4 096 C. We assume that macronuclear DNA may be present only once per haploid genome. In this case it represents only 1.6% of the DNA in micronuclei or 10% of the DNA in the giant chromosome stage.
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Keywords {🔍}

dna, google, scholar, article, stylonychia, biol, ammermann, hennig, mytilus, repetitive, chromosomes, sequences, chromosoma, macronucleus, molec, content, berger, genome, macronuclear, privacy, cookies, development, ciliated, steinbrück, ludwig, protozoa, micronuclei, publish, search, dieter, günther, wolf, gang, kinetic, complexity, access, cell, berl, data, information, log, journal, research, protozoan, times, haploid, coli, replication, giant, developmental,

Topics {✒️}

von berger month download article/chapter ploidy level order reaction subchromosomal-size dna molecules drosophila hydei ii giant chromosome stage nucleic acid metabolism privacy choices/manage cookies related subjects full article pdf dna breakdown products highly repetitive sequences haploid genome european economic area article chromosoma aims impaired nuclear functions small nuclear packages über desoxyribonucleinsäure-molekeln stylonychia mytilus exconjugants ciliates stylonychia mytilus conditions privacy policy repetitive dna sequences subsequent disintegration phase accepting optional cookies vegetative polyploid macronuclei check access instant access reaction rate main content log ulm journal finder publish macronuclear dna reassociates ciliated protozoa article ammermann replication phase hennig protozoan stylonychia 5 replication steps article log stylonychia mytilus privacy policy personal data genome instability article cite books a repetitive dna �repetitive” dna repeated sequences optional cookies

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         headline:The development of the macronucleus in the ciliated protozoan Stylonychia mytilus
         description:Ciliated protozoa are characterized by generative micronuclei and vegetative polyploid macronuclei. Micronuclei of Stylonychia mytilus contain 1 600 times as much DNA per haploid genome as E. coli. Most of this DNA is shown to be repetitive. The development of the macronucleus involves, as demonstrated by cytology, only 1/3 of the chromosomes which in a first replication phase are polytenized in probably 5 replication steps and appear as giant chromosomes. At this developmental stage considerable amounts of repetitive DNA are still present in the chromosomes. During the subsequent disintegration phase more than 90% of the DNA are eliminated from the macronucleus anlage. The remainder is further replicated five times and composes the final macronucleus. Since this DNA reassociates with a reaction rate almost identical to an ideal second order reaction its kinetic complexity can be determined by comparison with the kinetic complexity of E. coli DNA. Macronuclear DNA reassociates with a kinetic complexity of 26 times the kinetic complexity of E. coli DNA (corrected for GC content) which indicates that macronuclear DNA sequences exist at a ploidy level of 4 096 C. We assume that macronuclear DNA may be present only once per haploid genome. In this case it represents only 1.6% of the DNA in micronuclei or 10% of the DNA in the giant chromosome stage.
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            Order Reaction
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            Animal Genetics and Genomics
            Eukaryotic Microbiology
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      headline:The development of the macronucleus in the ciliated protozoan Stylonychia mytilus
      description:Ciliated protozoa are characterized by generative micronuclei and vegetative polyploid macronuclei. Micronuclei of Stylonychia mytilus contain 1 600 times as much DNA per haploid genome as E. coli. Most of this DNA is shown to be repetitive. The development of the macronucleus involves, as demonstrated by cytology, only 1/3 of the chromosomes which in a first replication phase are polytenized in probably 5 replication steps and appear as giant chromosomes. At this developmental stage considerable amounts of repetitive DNA are still present in the chromosomes. During the subsequent disintegration phase more than 90% of the DNA are eliminated from the macronucleus anlage. The remainder is further replicated five times and composes the final macronucleus. Since this DNA reassociates with a reaction rate almost identical to an ideal second order reaction its kinetic complexity can be determined by comparison with the kinetic complexity of E. coli DNA. Macronuclear DNA reassociates with a kinetic complexity of 26 times the kinetic complexity of E. coli DNA (corrected for GC content) which indicates that macronuclear DNA sequences exist at a ploidy level of 4 096 C. We assume that macronuclear DNA may be present only once per haploid genome. In this case it represents only 1.6% of the DNA in micronuclei or 10% of the DNA in the giant chromosome stage.
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      dateModified:
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      pageEnd:429
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         Order Reaction
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         general
         Human Genetics
         Animal Genetics and Genomics
         Eukaryotic Microbiology
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