We are analyzing https://link.springer.com/article/10.1007/bf00053902.

Title:
Morphotypic plasticity in vitro and in nude mice of epithelial mouse mammary cells (NMuMG) displaying an epithelioid (e) or a fibroblastic (f) morphotype in culture | Clinical & Experimental Metastasis
Description:
Transition from an epithelioid (e-) to a fibroblastic (f-) morphotype marks invasiveness in clinical and experimental cancer. To understand better the factors influencing such transitions, we have subcloned and manipulated mouse mammary gland (NMuMG) cell cultures and compared the invasive phenotype of multiple subclones in vitro and in vivo. Cell lines with an e-morphotype expressed E-cadherin homogeneously and were not invasive in vitro. Cells with an f-morphotype were E-cadherin-negative and became fully invasive in vitro upon expression of the ras oncogene. Invasive tumors were produced in nude mice after subcutaneous injection of e-type or f-type cells. These tumors showed cystic, glandular and undifferentiated structures. Tumors from f-type cells were E-cadherin-negative whereas e-type tumors stained heterogeneously in immunohistochemical preparations. Our observations demonstrate the impact of the micro-ecosystem on the invasive phenotype, with in vivo downregulation of E-cadherin and stimulation of the e- to f-morphotype transition.
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Keywords {🔍}

google, scholar, cell, cells, cancer, van, mammary, mareel, invasion, vitro, metastasis, epithelial, ecadherin, article, mouse, roy, invasive, tumor, biol, mice, culture, bruyne, expression, tumors, access, res, privacy, cookies, content, experimental, vleminckx, vivo, bracke, natl, sci, publish, research, search, clinical, nude, vakaet, transition, breast, molecular, adhesion, int, carcinomas, rat, progression, bruyneel,

Topics {✒️}

month download article/chapter hormone-dependent adenocarcinoma cells e-cadherin gene transcript mouse mammary carcinoma crit rev oncogenesis invasive collective migration mouse mammary cells epithelial cell plasticity heparan sulfate-rich breast tumor microenvironment f-morphotype transition related subjects full article pdf van den berghe privacy choices/manage cookies morphotypic plasticity invasion suppressor role van roy fm spindle cell carcinomas e-cadherin-negative epithelial cell cultures tumor cells metastasize natl cancer inst tumor-cell dissociation tumors showed cystic monoclonal antibodies specific cancer metastasis rev gastric cancer patients n-cadherin malignant fibrosarcoma cells leen van hoorde van der schueren clin exp metastasis invasive tumor cells martinus nijhoff publishers embryonic chick heart basal lamina formation e-cadherin expression f-type cells van cauwenberge rm trends cell biol semin cell biol mol cell biol european economic area blood group antigens intermediate filament proteins fabre monograph series hum mol genet transfected genomic dna cell type heterogeneity

Questions {❓}

Mareel MM, De Bruyne GK, Sonnenberg A and Hilgers J, 1986, Experimental results on invasiveness of mouse mammary cells: clinical implications?
Mareel MM, Van Roy FM and Bracke ME, 1993, How and when do tumor cells metastasize?

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         description:Transition from an epithelioid (e-) to a fibroblastic (f-) morphotype marks invasiveness in clinical and experimental cancer. To understand better the factors influencing such transitions, we have subcloned and manipulated mouse mammary gland (NMuMG) cell cultures and compared the invasive phenotype of multiple subclones in vitro and in vivo. Cell lines with an e-morphotype expressed E-cadherin homogeneously and were not invasive in vitro. Cells with an f-morphotype were E-cadherin-negative and became fully invasive in vitro upon expression of the ras oncogene. Invasive tumors were produced in nude mice after subcutaneous injection of e-type or f-type cells. These tumors showed cystic, glandular and undifferentiated structures. Tumors from f-type cells were E-cadherin-negative whereas e-type tumors stained heterogeneously in immunohistochemical preparations. Our observations demonstrate the impact of the micro-ecosystem on the invasive phenotype, with in vivo downregulation of E-cadherin and stimulation of the e- to f-morphotype transition.
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      headline:Morphotypic plasticity in vitro and in nude mice of epithelial mouse mammary cells (NMuMG) displaying an epithelioid (e) or a fibroblastic (f) morphotype in culture
      description:Transition from an epithelioid (e-) to a fibroblastic (f-) morphotype marks invasiveness in clinical and experimental cancer. To understand better the factors influencing such transitions, we have subcloned and manipulated mouse mammary gland (NMuMG) cell cultures and compared the invasive phenotype of multiple subclones in vitro and in vivo. Cell lines with an e-morphotype expressed E-cadherin homogeneously and were not invasive in vitro. Cells with an f-morphotype were E-cadherin-negative and became fully invasive in vitro upon expression of the ras oncogene. Invasive tumors were produced in nude mice after subcutaneous injection of e-type or f-type cells. These tumors showed cystic, glandular and undifferentiated structures. Tumors from f-type cells were E-cadherin-negative whereas e-type tumors stained heterogeneously in immunohistochemical preparations. Our observations demonstrate the impact of the micro-ecosystem on the invasive phenotype, with in vivo downregulation of E-cadherin and stimulation of the e- to f-morphotype transition.
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         Hematology
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