DOI . ORG {}
Title[redir]:
Bcl-rambo, a Novel Bcl-2 Homologue That Induces Apoptosis via Its Unique C-terminal Extension* - Journal of Biological Chemistry
Description:
The Bcl-2 family of proteins plays a central regulatory role in apoptosis. We have identified a novel, widely expressed Bcl-2 member which we have named Bcl-rambo. Bcl-rambo shows overall structural homology to the anti-apoptotic Bcl-2 members containing conserved Bcl-2 homology (BH) motifs 1, 2, 3, and 4. Unlike Bcl-2, however, the C-terminal membrane anchor region is preceded by a unique 250 amino acid insertion containing two tandem repeats. No interaction of Bcl-rambo with either anti-apoptotic (Bcl-2, Bcl-xL, Bcl-w, A1, MCL-1, E1B-19K, and BHRF1) or pro-apoptotic (Bax, Bak, Bik, Bid, Bim, and Bad) members of the Bcl-2 family was observed.
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Keywords {🔍}
bclrambo, bcl, cell, cells, members, domain, death, fig, apoptosis, protein, membrane, activity, family, region, caspase, expression, google, scholar, primer, scopus, pubmed, cterminal, proapoptotic, bhno, text, antibody, transfected, full, homology, anchor, data, human, figure, proteins, shown, pdf, bclxl, bax, mitochondria, sequence, min, molecular, presence, antiapoptotic, mitochondrial, pcr, transfection, crossref, bak, fulllength,
Topics {✒️}
pan-caspase inhibitor z-vad-fmk peroxidase-coupled goat anti-mouse calcium phosphate/bes method membrane-anchored c-terminal domain calcium phosphate/hepes method construct full-length bcl-rambo asbmb publications journal vsv-tagged bcl-2 anti-flag-m2-agarose beads c-terminal membrane anchor bcl-rambo-mediated cell death flag-tagged bcl-rambo constructs pan-caspase-inhibitor zvad-fmk bcl-rambo-induced cell death full-length bcl-rambo lacking pcmv-β-galactosidase expression vector bcl-rambo-induced caspase-3 activation random-primed radioactive probes n-terminal 1–200-amino acid anti-bcl-rambo antibody al167 anti-rabbit secondary antibodies lesser extend c-iap1 anti-flag m2-agarose n-terminal flag epitope endoplasmic reticulum/nuclear envelope current protocols p53-induced protein p53aip1 bcl-ramboδbhno induced apoptosis full-length bcl-rambo anti-flag antibody m2 reaction buffer antibody detecting smac/diablo c-terminal transmembrane domain bcl-rambo significantly decreased optimal pro-apoptotic activity �mitochondrial’ pro-apoptotic pathway c-terminal 255–485-aa region human bcl-rambo gene unique 250-aa-long region anti-smac/diablo antibodies pro-apoptotic bcl-2 member pro-apoptotic family member hydrophobic c-terminal segment iap inhibitor smac/diablo anti-apoptotic bcl-2 members complete bcl-rambo gene pro-apoptotic bcl-2 members c-terminal bhno domain bcl-rambo expression plasmids flag-tagged bcl-xl
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