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We began analyzing https://www.nature.com/articles/s41598-017-00186-9, but it redirected us to https://www.nature.com/articles/s41598-017-00186-9. The analysis below is for the second page.

Title[redir]:
Methionine aminopeptidase 2 is a key regulator of apoptotic like cell death in Leishmania donovani | Scientific Reports
Description:
We investigate the role of methionine aminopeptidase 2 (MAP2) in miltefosine induced programmed cell death (PCD) in promastigote form of L. donovani. We report that TNP-470, an inhibitor of MAP2, inhibits programmed cell death in miltefosine treated promastigotes. It inhibits the biochemical features of metazoan apoptosis, including caspase3/7 protease like activity, oligonucleosomal DNA fragmentation, collapse of mitochondrial transmembrane potential, and increase in cytosolic pool of calcium ions but did not prevent the cell death and phosphatidyl serine externalization. The data suggests that the MAP2 is involved in the regulation of PCD in parasite. Moreover, TNP-470 shows the leishmanicidal activity (IC50 = 15 µM) and in vitro inhibition of LdMAP2 activity (K i  = 13.5 nM). Further studies on MAP2 and identification of death signaling pathways provide valuable information that could be exploited to understand the role of non caspase proteases in PCD of L. donovani.

Matching Content Categories {📚}

  • Science
  • Education
  • Telecommunications

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🏙️ Massive Traffic: 50M - 100M visitors per month


Based on our best estimate, this website will receive around 76,749,346 visitors per month in the current month.

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How Does Doi.org Make Money? {💸}

We're unsure how the site profits.

Not all websites focus on profit; some are designed to educate, connect people, or share useful tools. People create websites for numerous reasons. And this could be one such example. Doi.org might be cashing in, but we can't detect the method they're using.

Keywords {🔍}

cells, cell, miltefosine, pubmed, treated, article, tnp, cas, google, scholar, death, leishmania, donovani, promastigotes, map, apoptosis, activity, dna, ldmap, apoptotic, induced, caspase, fig, promastigote, control, fragmentation, nature, protease, parasite, inhibitor, inhibition, role, reported, analysis, methionine, assay, fluorescence, increase, protein, dubey, pcd, membrane, genomic, buffer, showed, kumar, concentration, dmso, potential, calcium,

Topics {✒️}

n-acetyl-asp-glu-val-asp-al nature portfolio author information authors research funding nature references das m199 media supplemented 0/ reprints author correspondence generate pet-28a-ldmap2 plasmid development alpha-acetyl transferase families ar-kr laser beam bt/555/ne/u-excel/2014 boc-d-fmk prevent steady-state kinetic characterization ni-nta affinity chromatography n-terminal methionine residue amino-terminal methionine processing confocal microscopy data caspase-independent mitochondrial toxicity vikash kumar dubey metal-substituted methionine aminopeptidase annexin v-fitc binds 50 mm tris-hcl buffer vitro betulin-induced apoptosis hypericin-induced parasite death media india miltefosine-unresponsive leishmania donovani broad spectrum calpain staurosporine-treated leishmania donovani calpain/cysteine protease inhibitors flow cytometric analysis ne laser beam s2 rights ni-nta purified ldmap2 facscalibur flow cytometer article kumar reactive oxygen species annexin v-fitc annexin-v-fitc pre-incubated reaction mixture caspase specific inhibitors17 anti leishmaniasis activity n-terminal processing leishmania results map2 inhibitors prevents lineweaver-burk plots lower membrane potential

Questions {❓}

  • Cell death in Leishmania induced by stress and differentiation: programmed cell death or necrosis?

Schema {🗺️}

WebPage:
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         headline:Methionine aminopeptidase 2 is a key regulator of apoptotic like cell death in Leishmania donovani
         description:We investigate the role of methionine aminopeptidase 2 (MAP2) in miltefosine induced programmed cell death (PCD) in promastigote form of L. donovani. We report that TNP-470, an inhibitor of MAP2, inhibits programmed cell death in miltefosine treated promastigotes. It inhibits the biochemical features of metazoan apoptosis, including caspase3/7 protease like activity, oligonucleosomal DNA fragmentation, collapse of mitochondrial transmembrane potential, and increase in cytosolic pool of calcium ions but did not prevent the cell death and phosphatidyl serine externalization. The data suggests that the MAP2 is involved in the regulation of PCD in parasite. Moreover, TNP-470 shows the leishmanicidal activity (IC50 = 15 µM) and in vitro inhibition of LdMAP2 activity (K i  = 13.5 nM). Further studies on MAP2 and identification of death signaling pathways provide valuable information that could be exploited to understand the role of non caspase proteases in PCD of L. donovani.
         datePublished:2017-03-07T00:00:00Z
         dateModified:2017-03-07T00:00:00Z
         pageStart:1
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         license:http://creativecommons.org/licenses/by/4.0/
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            Humanities and Social Sciences
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      headline:Methionine aminopeptidase 2 is a key regulator of apoptotic like cell death in Leishmania donovani
      description:We investigate the role of methionine aminopeptidase 2 (MAP2) in miltefosine induced programmed cell death (PCD) in promastigote form of L. donovani. We report that TNP-470, an inhibitor of MAP2, inhibits programmed cell death in miltefosine treated promastigotes. It inhibits the biochemical features of metazoan apoptosis, including caspase3/7 protease like activity, oligonucleosomal DNA fragmentation, collapse of mitochondrial transmembrane potential, and increase in cytosolic pool of calcium ions but did not prevent the cell death and phosphatidyl serine externalization. The data suggests that the MAP2 is involved in the regulation of PCD in parasite. Moreover, TNP-470 shows the leishmanicidal activity (IC50 = 15 µM) and in vitro inhibition of LdMAP2 activity (K i  = 13.5 nM). Further studies on MAP2 and identification of death signaling pathways provide valuable information that could be exploited to understand the role of non caspase proteases in PCD of L. donovani.
      datePublished:2017-03-07T00:00:00Z
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